The cell wall porin of Nocardia farcinica: Biochemical identification of the channel-forming protein and biophysical characterization of the channel properties

Molecular Microbiology

Volumn 29, Issue 1, 1998, Pages 139-150

  Rieß, Franziska G ^a   Lichtinger, Thomas ^a   Cseh, Richard ^a   Yassin, Atteyet F ^b   Schaal, Klaus P ^b   Benz, Roland ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

^b UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

PORIN;

ACTINOBACTERIA; ARTICLE; BACTERIAL CELL WALL; BILAYER MEMBRANE; CELL WALL; CONDUCTANCE; CORYNEBACTERIUM; GRAM POSITIVE BACTERIUM; LIPID BILAYER; MYCOBACTERIUM; NOCARDIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN PURIFICATION;

ACTINOBACTERIA (CLASS); ACTINOMYCETALES; BACTERIA (MICROORGANISMS); CORYNEBACTERIA; CORYNEBACTERINEAE; CORYNEBACTERIUM; MYCOBACTERIUM; NOCARDIA FARCINICA; POSIBACTERIA;

EID: 0031824098     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.00914.x     Document Type: Article

References (54)

1. Barksdale, L. (1981) The genus Corynebacterium. In The Prokaryotes. Starr, M.P., Stoll, H., Trüper, H.G., Balows, A., and Schlegel, H.G. (eds). Berlin: Springer-Verlag, pp. 1827-1837.
2. Beamen, B.L., and Beamen, L. (1994) Nocardia species: host-parasite relationships. Clin Microbiol Rev 7: 213-264.
3. Beamen, B.L., Boiron, P., Beaman, L., Brownell, G.H., Schall, K.P., and Gombert, M.E. (1992) Nocardia and Nocardiosis. J Med Vet Mycol 30: 317-331.
4. Benz, R. (1988) Structure and function of porins from Gram-negative bacteria. Ann Rev Microbiol 42: 359-393.
5. Benz, R. (1994) Solute uptake through bacterial outer membranes. In Bacterial Cell Wall. Hackenbek, R., and Ghuysen, J.-M. (eds). Amsterdam: Elsevier, pp. 397-423.
6. Benz, R., Janko, K., Boos, W., and Läuger, P. (1978) Formation of large, ion-permeable membrane channels by the matrix protein (porin) of Escherichia coli. Biochim Biophys Acta 511: 305-319.
7. Benz, R., Janko, K., and Läuger, P. (1979) Ionic selectivity of pores formed by the matrix protein (porin) of Escherichia coli. Biochim Biophys Acta 551: 238-247.
8. Benz, R., Hardie, K.R., and Hughes, C. (1994) Pore formation in artificial membranes by the secreted hemolysins of Proteus vulgaris and Morganella morganii. Eur J Biochem 220: 339-347.
9. Blümel, J., Blümel, E., Yassin, A.-F., Schmidt-Rotte, H., and Schaal, K.P. (1998) Typing of Nocardia farcinica by pulsed-field gel electrophoresis reveals an endemic strain as source of hospital infections. J Clin Microbiol 36: 118-122.
10. Boiron, P., Provost, F., Chevrier, G., and Dupont, B. (1992) Review of nocardial infections in France 1987-90. Eur J Clin Microbiol Infect Dis 11: 709-714.
11. Brennan, P.J., and Nikaido, H. (1995) The envelope of mycobacteria. Annu Rev Biochem 64: 29-63.
12. Castellan, G.W. (1983) The ionic current in aqueous solutions. In Physical Chemistry. Reading, MA: Addison-Wesley, pp. 769-780.
13. Chatterjee, D., Bozic, C.M., McNeil, M., and Brennan, P.J. (1991) Structural features of the arabinan component of the lipoarabinomannan of Mycobacterium tuberculosis. J Biol Chem 266: 9652-9660.
14. Daffe, M., Brennan, P.J., and McNeil, M. (1990) Predominant structural features of the ceil wall arabinogalactan of Mycobacterium tuberculosis as revealed through characterisation of oligoglycosyl alditol fragments by gas chromatography/mass spectrometry and by NMR-analyses. J Biol Chem 265: 6734-6743.
15. Goodfellow, M., and Minnikin, D.E. (1977) Nocardioform bacteria. Ann Rev Microbiol 33: 159-180.
16. Goodfellow, M., Collins, M.D., and Minnikin, D.E. (1976) Thin-layer Chromatographic analysis of mycolic acid and other long-chain components in whole organism methanolysates of Coryneform and related taxa. J Gen Microbiol 96: 351-358.
17. Gross, H.J., Baier, H., and Blum, H. (1987) Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-99.
18. Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T., and Williams, ST. (1994) (eds) Nocardioform Actinomycetes. In Bergey's Manual of Determinative Biology. Baltimore USA: Williams & Wilkins, pp. 625-650.
19. Jarlier, V., and Nikaido, H. (1990) Permeability barrier to hydrophilic solutes in Mycobacterium chelonae. J Bacteriol 172: 1418-1423.
20. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
21. Lichtinger, T., Niederweis, M., Krämer, R., and Benz, R. (1998) Biochemical and biophysical characterization of the cell wall channel of Corynebacterium glutamicum: the channel is formed by a low molecular mass subunit. Biochemistry (submitted).
22. Liu, J., Rosenberg, E.Y., and Nikaido, H. (1995) Fluidity of the lipid domain of cell wall from Mycobacterium chelonae. Proc Natl Acad Sci USA 92: 11254-11258.
23. Liu, J., Barry III, C.E., Besra, G.S., and Nikaido, H. (1996) Mycolic acid structure determines the fluidity of the mycobacterial cell wall. J Biol Chem 271: 29545-29551.
24. Ludwig, O., De Pinto, V., Palmieri, F., and Benz, R. (1986) Pore formation by the mitochondrial porin of rat brain in lipid bilayer membranes. Biochim Biophys Acta 860: 268-276.
25. Maier, E., Reinhard, N., Benz, R., and Frey, J. (1996) Channel-forming activity and channel size of the RTX-toxins Apxl, Apxll, and Apxlll of Actinobacillus pleuropnomoniae. Infect Immun 64: 4415-4423.
26. Menestrina, R., and Antolini, R. (1981) Ion transport through the hemocyanin channels in oxidized cholesterol membranes. Biochim Biophys Acta 643: 616-625.
27. Minnikin, D.E. (1982) Lipids: Complex lipids, their chemistry, biosynthesis and roles. In The Biology of the Mycobacteria. Ratledge, C., and Stanford, J.C. (eds). New York: Academic Press, pp. 95-184.
28. Minnikin, D.E. (1987) Chemical targets in cell envelopes. In Chemotherapy of Tropical Diseases. Hopper, M. (eds). Chichester: John Wiley and Sons, pp. 19-43.
29. Minnikin, D.E. (1991) Chemical principles in the organization of lipid components in the mycobacterial cell envelope. Res Microbiol 142: 423-427.
30. Minnikin, D.E., Patel, P.V., and Goodfellow, M. (1974) Mycolic acids of representative strains of Nocardia and the 'rhodochrous' complex. FEBS Lett 39: 322-324.
31. Nelson, A.P., and McQuarrie, D.A. (1975) The effect of discrete charges on the electrical properties of the membrane. J Theor Biol 55: 13-27.
32. Niederweis, M., Maier, E., Lichtinger, T., Benz, R., and Krämer, R. (1995) Identification of channel-forming activity in the cell wall of Corynebacterium glutamicum. J Bacteriol 177: 5716-5718.
33. Nikaido, H. (1992) Porins and specific channels of bacterial outer membranes. Mol Microbiol 6: 435-442.
34. Nikaido, H., and Vaara, M. (1985) Molecular basis of bacterial outer membrane permeability. Microbiol Rev 49: 1-32.
35. Nikaido, H., and Jarlier, V. (1991) Permeability of the mycobacterial cell wall. Res Microbiol 142: 437-443.
36. Nikaido, H., and Rosenberg, E.Y. (1981) Effect of solute size on diffusion rates through the transmembrane pores of the outer membrane of Escherichia coli. J Gen Physiol 77: 121-135.
37. Nikaido, H., Kim, S.H., and Rosenberg, E.Y. (1993) Physical organization of lipids in the cell wall of Mycobacterium chelonae. Mol Microbiol 8: 1025-1030.
38. Ochi, K. (1995) Phylogenetic analysis of mycolic acid-containing wall-chemotype IV actinomycetes and allied taxa by partial sequencing of ribosomal protein AT-L30. Int J Syst Bacteriol 45: 653-660.
39. Renkin, E.M. (1954) Filtration, diffusion, and molecular sieving through porous cellulose membranes. J Gen Physiol 38: 225-243.
40. Schaal, K.P., and Lee, H.J. (1992) Actinomycete infections in humans - a review. Gene 115: 201-211.
41. Schaal, K.P., and Leischik, W. (1969) Zur Antibiotikatheraphie der Nocardiose. Dtsch med Wschr 94: 2505-2507.
42. Schaal, K.P., Schütt-Gerowitt, H., and Goldmann, A. (1986) In vitro and in vivo studies on the efficacy of various antimicrobial agents in the treatment of human nocardiosis. In Biological, Biochemical, and Biomedical Aspects of Actinomycetes. Szabo, G., Biro, S., and Goodfellow, M. (eds). Budapest: Academiai Kiado, pp. 619-633.
43. Schägger, H., and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 KDa. Anal Biochem 166: 368-379.
44. Schindler, H., and Rosenbusch, J.P. (1981) Matrix protein in planar membranes; clusters of channels in a native environment and their functional reassembly. Proc Natl Acad Sci USA 78: 2302-2306.
45. Schleifer, K., and Kandler, O. (1967) Zur chemischen Zusammensetzung der Zellwand der Streptokokken. Archiv für Mikrobiologie 57: 335-364.
46. Schmid, A., Krömer, S., Heldt, H.W., and Benz, R. (1992) Identification of two general diffusion channels in the outer membrane of pea mitochondria. Biochim Biophys Acta 1112: 174-180.
47. Sen, K., Hellman, J., Nikaido, H. (1988) Porin channels in intact cells of Escherichia coli are not affected by Donnan potentials across the outer membrane. J Biol Chem 263: 1182-1187.
48. Stackebrandt, E., Rainey, F.A., and Ward-Rainey, N.L. (1997) Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47: 479-491.
49. Trias, J., and Benz, R. (1993) Characterization of the channel formed by the mycobacterial porin of Mycobacterium chelonae in lipid-bilayer membranes: demonstration of voltage dependent regulation and the presence of negative point charges at the channel mouth. J Biol Chem 268: 6234-6240.
50. Trias, J., and Benz, R. (1994) Permeability of the cell wall of Mycobacterium smegmatis. Mol Microbiol 14: 283-290.
51. Trias, J., Jarlier, V., and Benz, R. (1992) Porins in the cell wall of mycobacteria. Science 258: 1479-1481.
52. Weiss, M.S., Kreusch, A., Schiltz, E., Nestel, U., Weite, W., Weckesser, J., and Schultz, G.E. (1991) The structure of porin from Rhodobacter capsulatus at 1.8 Å resolution. FEBS Lett 280: 379-382.
53. Yano, I., and Saito, K. (1972) Gas Chromatographic and mass spectrometric analysis of molecular species of corynomycolic acids from Corynebacterium ulcerans. FEBS Lett 23: 352-356.
54. Yassin, A.F., Rainey, F.A., Brzezinka, H., Burghardt, J., Schmitt, S., Seifert, P., et al. (1997) Tsukamurella tyrosinosolvens sp. nov. Int J Syst Bacteriol 47: 607-614.