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Volumn 120, Issue 1, 1998, Pages 205-216

Pyruvate dehydrogenase E1α isoform in rat testis: cDNA cloning, characterization, and biochemical comparison of the recombinant testis and liver enzymes

Author keywords

cDNA cloning; Gene expression; Phosphorylation; Pyruvate dehydrogenase; Rat testis; Recombinant protein characterization; Sequence variation; Tissue specific isozyme

Indexed keywords

COMPLEMENTARY DNA; LIVER ENZYME; PYRUVATE DEHYDROGENASE; PYRUVATE DEHYDROGENASE COMPLEX; RECOMBINANT ENZYME;

EID: 0031822479     PISSN: 03050491     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0305-0491(98)10010-X     Document Type: Article
Times cited : (7)

References (44)
  • 1
    • 0017143546 scopus 로고
    • Studies on the formation of acetoin from acetaldehyde by the pyravate dehydrogenase complex and its regulation
    • Alkonyl I, Bolygo E, Gyocsi L, Szabo D. Studies on the formation of acetoin from acetaldehyde by the pyravate dehydrogenase complex and its regulation. Eur J Biochem 1976;66:551-7.
    • (1976) Eur J Biochem , vol.66 , pp. 551-557
    • Alkonyl, I.1    Bolygo, E.2    Gyocsi, L.3    Szabo, D.4
  • 3
    • 0025083820 scopus 로고
    • Pyruvate dehydrogenase E1α subunit genes in the mouse: Mapping and comparison with human homologs
    • Brown RM, Dahl H-HM, Brown GK. Pyruvate dehydrogenase E1α subunit genes in the mouse: Mapping and comparison with human homologs. Somatic Cell Mol Genet 1990;16:487-92.
    • (1990) Somatic Cell Mol Genet , vol.16 , pp. 487-492
    • Brown, R.M.1    Dahl, H.-H.M.2    Brown, G.K.3
  • 4
    • 0023461995 scopus 로고
    • The measurement of 1,2-propanediol,D,L-2,3-butanediol and meso-2,3-butanediol in controls and alcoholic cirrhotics
    • Casazza JP, Frietas J, Stambuk D, Morgan MY, Veech RL. The measurement of 1,2-propanediol,D,L-2,3-butanediol and meso-2,3-butanediol in controls and alcoholic cirrhotics. Alcohol 1987;22(Suppl. 1):607-9.
    • (1987) Alcohol , vol.22 , Issue.1 SUPPL. , pp. 607-609
    • Casazza, J.P.1    Frietas, J.2    Stambuk, D.3    Morgan, M.Y.4    Veech, R.L.5
  • 5
    • 0022860850 scopus 로고
    • Isolation of the gene for the testis-specific H1 histone variant Hit
    • Cole KD, Kandala JC, Kistler WS. Isolation of the gene for the testis-specific H1 histone variant Hit. J Biol Chem 1986;261:7178-83.
    • (1986) J Biol Chem , vol.261 , pp. 7178-7183
    • Cole, K.D.1    Kandala, J.C.2    Kistler, W.S.3
  • 6
    • 0027358586 scopus 로고
    • Characterization of cD-NAs encoding the rat testis-specific E1α subunit of the pyruvate dehydrogenase complex: Comparison of expression of the corresponding mRNA with that of the somatic E1α subunit
    • Cullingford TE, Clark JB, Phillips IR. Characterization of cD-NAs encoding the rat testis-specific E1α subunit of the pyruvate dehydrogenase complex: Comparison of expression of the corresponding mRNA with that of the somatic E1α subunit. Biochim Biophys Acta 1993;1216:149-53.
    • (1993) Biochim Biophys Acta , vol.1216 , pp. 149-153
    • Cullingford, T.E.1    Clark, J.B.2    Phillips, I.R.3
  • 7
    • 0025049307 scopus 로고
    • A testis-specific form of the human pyruvate dehydrogenase E1α subunit is coded for by an intronless gene on chromosome 4
    • Dahl H-HM, Brown RM, Hutchison WM, Maragos C, Brown GK. A testis-specific form of the human pyruvate dehydrogenase E1α subunit is coded for by an intronless gene on chromosome 4. Genomics 1990;8:225-32.
    • (1990) Genomics , vol.8 , pp. 225-232
    • Dahl, H.-H.M.1    Brown, R.M.2    Hutchison, W.M.3    Maragos, C.4    Brown, G.K.5
  • 8
    • 0025142115 scopus 로고
    • Pyruvate dehydrogenase deficiency caused by deletion of a 7-bp repeat sequence in the E1α gene
    • Dahl H-HM, Maragos C, Brown RM, Hutchison WM, Brown GK. Pyruvate dehydrogenase deficiency caused by deletion of a 7-bp repeat sequence in the E1α gene. Am J Hum Genet 1990;47:286-93.
    • (1990) Am J Hum Genet , vol.47 , pp. 286-293
    • Dahl, H.-H.M.1    Maragos, C.2    Brown, R.M.3    Hutchison, W.M.4    Brown, G.K.5
  • 10
    • 0018816264 scopus 로고
    • The presence of 2,3-butanediol in the blood of chronic alcoholics admitted to an alcohol treatment center
    • Thurman RG, editor. New York: Plenum
    • Felver ME, Lakshmanan MR, Wolf S, Veech RL. The presence of 2,3-butanediol in the blood of chronic alcoholics admitted to an alcohol treatment center. In: Thurman RG, editor. Alcohol and Aldehyde Metabolizing Systems IV. New York: Plenum, 1980:229-35.
    • (1980) Alcohol and Aldehyde Metabolizing Systems IV , pp. 229-235
    • Felver, M.E.1    Lakshmanan, M.R.2    Wolf, S.3    Veech, R.L.4
  • 11
    • 0027114115 scopus 로고
    • Isolation and characterisation of the mouse pyruvate dehydrogenase E1α genes
    • Fitzgerald J, Hutchison WM, Dahl H-HM. Isolation and characterisation of the mouse pyruvate dehydrogenase E1α genes. Biochim Biophys Acta 1992;1131:83-90.
    • (1992) Biochim Biophys Acta , vol.1131 , pp. 83-90
    • Fitzgerald, J.1    Hutchison, W.M.2    Dahl, H.-H.M.3
  • 12
    • 0010500947 scopus 로고
    • Carbohydrate metabolism in the testis
    • Johnson AD, Gomes WR, Vandemark NL, editors. New York: Academic Press
    • Free M. Carbohydrate metabolism in the testis. In: Johnson AD, Gomes WR, Vandemark NL, editors. The Testis II. New York: Academic Press, 1970:125-92.
    • (1970) The Testis II , pp. 125-192
    • Free, M.1
  • 14
    • 0025757299 scopus 로고
    • Characterization of the mutations in three patients with pyruvate dehydrogenase E1α deficiency
    • Hansen LL, Brown GK, Kirby DM, Dahl H-HM. Characterization of the mutations in three patients with pyruvate dehydrogenase E1α deficiency. J Inhert Metab Dis 1991;14:140-51.
    • (1991) J Inhert Metab Dis , vol.14 , pp. 140-151
    • Hansen, L.L.1    Brown, G.K.2    Kirby, D.M.3    Dahl, H.-H.M.4
  • 15
    • 13144304728 scopus 로고
    • Immunologically distinct isoform of pyruvate dehydrogenase E1α subunit in rat testis
    • Abstract, New Orleans, LA
    • Huh TL, Casazza JP, Sohn DH, Veech RL, Song BJ. Immunologically distinct isoform of pyruvate dehydrogenase E1α subunit in rat testis. ASBMB meeting Abstract, New Orleans, LA, 1990.
    • (1990) ASBMB Meeting
    • Huh, T.L.1    Casazza, J.P.2    Sohn, D.H.3    Veech, R.L.4    Song, B.J.5
  • 16
    • 0026753356 scopus 로고
    • Transcriptional expression of a testis-specific variant of the mouse pyruvate dehydrogenase E1α subunit
    • Iannello RC, Dahl H-HM. Transcriptional expression of a testis-specific variant of the mouse pyruvate dehydrogenase E1α subunit. Biol Reprod 1992;47:48-58.
    • (1992) Biol Reprod , vol.47 , pp. 48-58
    • Iannello, R.C.1    Dahl, H.-H.M.2
  • 17
    • 0027989306 scopus 로고
    • Production of an enzymatically active El component of human pyruvate dehydrogenase complex in Escherichia coli: Supporting role of E1β subunit in E1 activity
    • Jeng J, Huh T-L, Song BJ. Production of an enzymatically active El component of human pyruvate dehydrogenase complex in Escherichia coli: Supporting role of E1β subunit in E1 activity. Biochem Biophys Res Commun 1994;203:225-30.
    • (1994) Biochem Biophys Res Commun , vol.203 , pp. 225-230
    • Jeng, J.1    Huh, T.-L.2    Song, B.J.3
  • 18
    • 0017624125 scopus 로고
    • Study of the kinetic mechanism of pyruvate-2,6-dichlorophenolindophenol reductase activity of muscle pyruvate dehydrogenase
    • Khailova LS, Bernhardt R, Huebner G. Study of the kinetic mechanism of pyruvate-2,6-dichlorophenolindophenol reductase activity of muscle pyruvate dehydrogenase. Biokhimiya 1977;42:113-7.
    • (1977) Biokhimiya , vol.42 , pp. 113-117
    • Khailova, L.S.1    Bernhardt, R.2    Huebner, G.3
  • 20
    • 0023665902 scopus 로고
    • An analysis of 5′-noncoding sequences from 699 vetebrate messenger RNAs
    • Kozak M. An analysis of 5′-noncoding sequences from 699 vetebrate messenger RNAs. Nucleic Acids Res 1987;15:8125-48.
    • (1987) Nucleic Acids Res , vol.15 , pp. 8125-8148
    • Kozak, M.1
  • 22
    • 0024362236 scopus 로고
    • Structural organization of the gene for the E1α subunit of the human pyruvate dehydrogenase complex
    • Maragos C, Hutchison WM, Hayasaka K, Brown GK, Dahl H-HM. Structural organization of the gene for the E1α subunit of the human pyruvate dehydrogenase complex. J Biol Chem 1989;264:12294-8.
    • (1989) J Biol Chem , vol.264 , pp. 12294-12298
    • Maragos, C.1    Hutchison, W.M.2    Hayasaka, K.3    Brown, G.K.4    Dahl, H.-H.M.5
  • 23
    • 0025868849 scopus 로고
    • The α-ketoacid dehydrogenase complexes. Sequence similarity of rat pyruvate dehydrogenase with Escherichia coli and Azotobacter vinelandii α-ketoglutarate dehydrogenase
    • Matuda S, Nakano K, Ohta S, Saheki T, Kawanishi Y-I, Miyatya T. The α-ketoacid dehydrogenase complexes. Sequence similarity of rat pyruvate dehydrogenase with Escherichia coli and Azotobacter vinelandii α-ketoglutarate dehydrogenase. Biochim Biophys Acta 1991;1089:1-7.
    • (1991) Biochim Biophys Acta , vol.1089 , pp. 1-7
    • Matuda, S.1    Nakano, K.2    Ohta, S.3    Saheki, T.4    Kawanishi, Y.-I.5    Miyatya, T.6
  • 24
    • 0023091937 scopus 로고
    • Human testis-specific PGK gene lacks introns and possesses characteristics of a processed gene
    • McCarrey JR, Thomas K. Human testis-specific PGK gene lacks introns and possesses characteristics of a processed gene. Nature 1987;326:501-5.
    • (1987) Nature , vol.326 , pp. 501-505
    • McCarrey, J.R.1    Thomas, K.2
  • 25
    • 0021991099 scopus 로고
    • Biochemical study in 28 children with lactic acidosis, in relation to Leigh's encephalomyelopathy
    • Miyabayashi S, Ito T, Narisawa K, Iinuma K, Tada K. Biochemical study in 28 children with lactic acidosis, in relation to Leigh's encephalomyelopathy. Eur J Pediatr 1985;143:278-83.
    • (1985) Eur J Pediatr , vol.143 , pp. 278-283
    • Miyabayashi, S.1    Ito, T.2    Narisawa, K.3    Iinuma, K.4    Tada, K.5
  • 26
    • 0024326881 scopus 로고
    • Tissue-specific forms of type IX collagen-proteoglycan arise from the use of two widely separated promoters
    • Nishimura I, Murangaki Y, Olsen BR. Tissue-specific forms of type IX collagen-proteoglycan arise from the use of two widely separated promoters. J Biol Chem 1989;264:20033-41.
    • (1989) J Biol Chem , vol.264 , pp. 20033-20041
    • Nishimura, I.1    Murangaki, Y.2    Olsen, B.R.3
  • 27
    • 0025221771 scopus 로고
    • Molecular biology and biochemistry of pyruvate dehydrogenase complexes
    • Patel MS, Roche TE. Molecular biology and biochemistry of pyruvate dehydrogenase complexes. FASEB J 1990;4:3224-33.
    • (1990) FASEB J , vol.4 , pp. 3224-3233
    • Patel, M.S.1    Roche, T.E.2
  • 29
    • 0028149769 scopus 로고
    • Molecular cloning of the p45 subunit of pyruate dehydrogenase kinase
    • Popov KM, Kedishvili NY, Zhao Y, Gudi R, Harris RA. Molecular cloning of the p45 subunit of pyruate dehydrogenase kinase. J Biol Chem 1994;269:29720-4.
    • (1994) J Biol Chem , vol.269 , pp. 29720-29724
    • Popov, K.M.1    Kedishvili, N.Y.2    Zhao, Y.3    Gudi, R.4    Harris, R.A.5
  • 30
    • 0027452147 scopus 로고
    • Primary structure of pyruvate dehydrogenase kinase establishes a new family of eukaryotic protein kinases
    • Popov KM, Kedishvili NY, Zhao Y, Shimomura Y, Crabb DW, Harris RA. Primary structure of pyruvate dehydrogenase kinase establishes a new family of eukaryotic protein kinases. J Biol Chem 1993;268:26602-6.
    • (1993) J Biol Chem , vol.268 , pp. 26602-26606
    • Popov, K.M.1    Kedishvili, N.Y.2    Zhao, Y.3    Shimomura, Y.4    Crabb, D.W.5    Harris, R.A.6
  • 31
    • 0022802645 scopus 로고
    • Fuel selection in animals
    • Randle PJ. Fuel selection in animals. Biochem Soc Trans 1986;14:799-806.
    • (1986) Biochem Soc Trans , vol.14 , pp. 799-806
    • Randle, P.J.1
  • 32
    • 0022311019 scopus 로고
    • Regulation of mammalian pyruvate and branch-chain α-keto acid dehydrogenase complexes by phosphorylation-dephosphorylation
    • Reed LJ, Damuni Z, Merryfield ML. Regulation of mammalian pyruvate and branch-chain α-keto acid dehydrogenase complexes by phosphorylation-dephosphorylation. Curr Top Cell Regul 1985;27:41-9.
    • (1985) Curr Top Cell Regul , vol.27 , pp. 41-49
    • Reed, L.J.1    Damuni, Z.2    Merryfield, M.L.3
  • 34
    • 9544245935 scopus 로고    scopus 로고
    • Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human
    • Rowles J, Scherer SW, Xi T, Majer M, Nickle DC, Rommens JM, Popov KM, Harris RA. Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human. J Biol Chem 1996;271:22376-82.
    • (1996) J Biol Chem , vol.271 , pp. 22376-22382
    • Rowles, J.1    Scherer, S.W.2    Xi, T.3    Majer, M.4    Nickle, D.C.5    Rommens, J.M.6    Popov, K.M.7    Harris, R.A.8
  • 35
    • 0026076018 scopus 로고
    • Molecular cloning of the DNA and expression and characterization of rat testes fructose-6-phosphate, 2-kinase: Fructose-2,6-bisphosphatase
    • Sakata J, Abe Y, Uyeda K. Molecular cloning of the DNA and expression and characterization of rat testes fructose-6-phosphate, 2-kinase: Fructose-2,6-bisphosphatase. J Biol Chem 1991;266:15764-70.
    • (1991) J Biol Chem , vol.266 , pp. 15764-15770
    • Sakata, J.1    Abe, Y.2    Uyeda, K.3
  • 37
    • 0019320814 scopus 로고
    • Role of multisite phosphorylation in the regulation of ox kidney pyruvate dehydrogenase complex
    • Sugden PH, Simister NE. Role of multisite phosphorylation in the regulation of ox kidney pyruvate dehydrogenase complex. FEBS Lett 1980;169:433-5.
    • (1980) FEBS Lett , vol.169 , pp. 433-435
    • Sugden, P.H.1    Simister, N.E.2
  • 38
    • 0026572430 scopus 로고
    • The expression pattern of the pyruvate dehydrogenase E1α subunit genes during spermatogenesis in adult mouse
    • Takakubo F, Dahl H-HM. The expression pattern of the pyruvate dehydrogenase E1α subunit genes during spermatogenesis in adult mouse. Exp Cell Res 1992;199:39-49.
    • (1992) Exp Cell Res , vol.199 , pp. 39-49
    • Takakubo, F.1    Dahl, H.-H.M.2
  • 39
    • 0024109360 scopus 로고
    • Rapid phage DNA isolation without the use of enzymes
    • Tsonis PA, Manes T. Rapid phage DNA isolation without the use of enzymes. BioTechniques 1988;6:950-1.
    • (1988) BioTechniques , vol.6 , pp. 950-951
    • Tsonis, P.A.1    Manes, T.2
  • 40
    • 0019348695 scopus 로고
    • Control of a secondary pathway of ethanol metabolism by differences in redox state: A story of the failure to arrest the Krebs cycle for drunkenness
    • Veech RL, Felver ME, Lakshmanan MR, Huang M-T. Control of a secondary pathway of ethanol metabolism by differences in redox state: A story of the failure to arrest the Krebs cycle for drunkenness. Curr Top Cell Regul 1981;18:151-79.
    • (1981) Curr Top Cell Regul , vol.18 , pp. 151-179
    • Veech, R.L.1    Felver, M.E.2    Lakshmanan, M.R.3    Huang, M.-T.4
  • 41
    • 0022731676 scopus 로고
    • Mitochondrial targeting sequences may form amphiphilic helices
    • von Heijne G. Mitochondrial targeting sequences may form amphiphilic helices. EMBO J 1986;5:1335-42.
    • (1986) EMBO J , vol.5 , pp. 1335-1342
    • Von Heijne, G.1
  • 44
    • 0025827079 scopus 로고
    • Molecular defects in the E1α subunit of the branched-chain α-ketoacid dehydrogenase complex that cause maple syrup urine disease
    • Zhang B, Zhao Y, Harris RA, Crabb DW. Molecular defects in the E1α subunit of the branched-chain α-ketoacid dehydrogenase complex that cause maple syrup urine disease. Mol Biol Med 1991;8:39-47.
    • (1991) Mol Biol Med , vol.8 , pp. 39-47
    • Zhang, B.1    Zhao, Y.2    Harris, R.A.3    Crabb, D.W.4


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