Lead exposure increases oxidative stress in serum deprived E14 mesencephalic cultures. Role of metallothionein and glutathione

Restorative Neurology and Neuroscience

Volumn 12, Issue 2-3, 1998, Pages 95-101

  Scortegagna, Marzia ^a   Chikhale, Elsbeth ^b   Hanbauer, Ingeborg ^a  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^b NATIONAL INSTITUTE ON AGING   (United States)

Author keywords

Glutathione; Lead; Metallothionein; Oxidative Stress; Primary Cultures; Serum Deprivation

Indexed keywords

GLUTATHIONE; LEAD; METALLOTHIONEIN;

ANIMAL CELL; ANTIOXIDANT ACTIVITY; ARTICLE; BRAIN NERVE CELL; CONTROLLED STUDY; IMMEDIATE EARLY GENE; MESENCEPHALON; NEUROTOXICITY; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RAT;

EID: 0031821678     PISSN: 09226028     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Aschner, M., Mullaney, K.J., Wagoner, D., Lash, L.H. and Kimelberg, H. K. Intracellular glutathione (GSH) levels modulate mercuric chloride (MC) and methylmercuric chloride (MeHgCl)-induced amino acid release from neonatal rat primary astrocyte cultures, Brain Res., 664 (1994) 133-140.
2. Aschner, M., Mullaney, K.J., Fehm, M.N., Wagoner, D.E. and Vitarella D. Astrocytes as potential modulators of mercuric chloride neurotoxicity, Cell. and Molec. Neurobiol., 14 (1995) 637-652.
3. Brewer, G.J. Serum-free B27/neurobasal medium supports differentiated growth of neurons from the striatum, substantia nigra, septum, cerebral cortex, cerebellum, and dentate gyrus, J. Neurosci. Res., 42 (1995) 674-683.
4. Chubatsu, L. S., Gennari, M. and Meneghini, R. Glutathione is the antioxidant responsible for resistance to oxidative stress in V79 Chinese hamster fibroblasts rendered resistant to cadmium, Chem. Biol. Interactions, 82 (1992) 99-110.
5. Deane, R. and Bradbury, M.W.B. Transport of lead-203 at the blood-brain barrier during short cerebrovascular perfusion in the rat, J. Neurochem., 54 (1990) 905-914.
6. Freedman, J.H., Ciriolo, M.R. and Peisach, J. The role of glutathione in copper metabolism and toxicity, J. Biol. Chem., 264 (1989) 5598-5605.
7. Goering P.L. Lead-protein interactions as a basis for lead toxicity, NeuroToxicology, 14 (1993) 45-60.
8. Goering, P.L. and Klaassen, C. D. Tolerance to cadmium-induced hepatotoxicity following cadmium pretreatment, Toxicol. Appl. Pharmacol., 74 (1984) 308-313.
9. Han, S-K., Mytilineou, C. and Cohen, G. L-DOPA up-regulates glutathione and protects mesencephalic cultures against oxidative stress, J. Neurochem., 66 (1996) 501-510.
10. Hidalgo, J., Campmany, L., Marti, O. and Armario, A. Metallothionein-I induction by stress in specific brain areas, Neurochem. Res., 16 (1991) 1145-1148.
11. Khelil, M., Rolland, B., Fages, C. and Tardy, M. Glutamine synthetase modulation in astrocyte cultures of different mouse brain areas, Glia, 3 (1990) 75-80.
12. Knoepfel, L., Steinkühler, C., Carri, M-T. and Rotilio, G. Role of zinc-coordination and of the glutathione redox couple in the redox susceptibility of human transcription factor Sp1, Biochem. Biophys. Res. Commun., 201 (1994) 871-877.
13. Levine, R.L., Williams, J.A., Stadtman, E.R. and Shacter, E. Carbonyl assays for determination of oxidatively modified proteins, Meth. Enzymol., 233 (1994) 346-357.
14. Maret, W. Oxidative metal release from metallothionein via zinc-thiol/disulfide interchange, Proc. Natl. Acad. Sci. USA, 91 (1994) 237-241.
15. Meister, A. Glutamine synthetase from mammalian tissues, Methods Enzymol., 113 (1985) 185-199.
16. Meister, A. Metabolism and function of glutathione. In D. Dolphine, R. Poulson and O.J. Avramovic (Eds.), Coenzymes and cofactors. Glutathione: chemical, biochemical and medical aspects, Wiley and Sons, New York, 1989, pp. 367.
17. Neal, J.W., Singhrao, S.K., Jasani, B. and Newman, G.R. Immunocytochemically detectable metallothionein is expressed by astrocytes in the ischaemic human brain, Neuropathol. and Appl Neurobiol., 22 (1996) 243-247.
18. O'Malley, E.K., Sieber, B-A., Black, I.B. and Dreyfus, C.F. Mesencephalic type astrocytes mediate the survival of substantia nigra neurons in culture, Brain Res., 582 (1992) 65-70.
19. Palmite,r R.D. Molecular Biology of metallothionein gene expression, Experientia Suppl., 52 (1987) 63-80.
20. Palmiter, R.D. Regulation of metallothionein genes by heavy metals appear to be mediated by a zinc-sensitive inhibitor that interacts with a constitutively active transcription factor MTF-1, Proc. Natl. Acad. Sci. USA, 91 (1994) 1219-1223.
21. Palmiter, R.D. Constitutive expression of metallothionein (MT)-III but not MT-1 inhibits growth when cells become zinc deficient, Toxicol. Appl. Pharmacol., 135 (1995) 139-146.
22. Raps, S.P., Lai, J.C.K., Hertz, L. and Cooper, J.L. Glutathione is present in high concentrations in cultured astrocytes but not in cultured neurons, Brain Res., 493 (1989) 398-401.
23. Rising, L., Vitarella, D., Kimelberg, H.K. and Aschner, M. Cadmium chloride-induced metallothionein expression in neonatal rat primary astrocyte cultures, Brain Res., 678 (1995) 91-98.
24. Sagara, J., Miura, K. and Bannai, S. Maintenance of neuronal glutathione by glial cells, J. Neurochem., 61 (1993) 1672-1676.
25. Scortegagna, M. and Hanbauer, I. Horse serum deprivation facilitates neuronal death elicited by Pb2+ in mesencephalic primary cultures, NeuroToxicol., 18 (1997) 331-340.
26. Scortegagna, M., Chikhale, E. and Hanbauer, I. Effect of lead on cytoskeletal proteins expressed in E14 mesencephalic primary cultures, Neurochem. Int., 32 (1997) 353-359.
27. Tietze, F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: application to mammalian blood and other tissue, Analyt. Biochem., 27 (1969) 502-522.
28. Tiffany-Castiglioni, E. Cell culture models for lead toxicity in neuronal and glial cells, NeuroToxicology, 14 (1993) 513-536.
29. Young, J.K., Garvey, J.S. and Huang, P.C. Glial immunoreactivity for metallothionein in the rat brain, Glia, 4 (1991) 602-620.
30. Yudkoff, M., Pleasure, D., Cregar, L., Lin, Z. P., Nissim, I., Stern, J. and Nissim, I. Glutathione turnover in cultured astrocytes: studies with [14N]glutamate. J. Neurochem., 55 (1990) 137-145.