Synaptic activity, induced rhythmic discharge patterns, and receptor subtypes in enriched primary cultures of embryonic rat motoneurones

Canadian Journal of Physiology and Pharmacology

Volumn 76, Issue 3, 1998, Pages 347-359

  Wallis, D I ^a   Elliott, P ^a   Foster, G A ^a   Stringer, B M J ^b  

^a CARDIFF UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Adenosine; Amino acid receptors; Culture; Embryonic rat motoneurones; Spinal cord

Indexed keywords

6 CYANO 7 NITRO 2,3 QUINOXALINEDIONE; ALPHA AMINO 3 HYDROXY 5 METHYL 4 ISOXAZOLEPROPIONIC ACID; BICUCULLINE; GLUTAMIC ACID; GLYCINE; KAINIC ACID; N METHYL DEXTRO ASPARTIC ACID; STRYCHNINE;

ACTION POTENTIAL; ANIMAL CELL; ARTICLE; CELL CULTURE; CONTROLLED STUDY; ELECTROPHYSIOLOGY; IN VITRO STUDY; MOTONEURON; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; SPINAL CORD; SYNAPTIC POTENTIAL;

EID: 0031820233     PISSN: 00084212     EISSN: None     Source Type: Journal    
DOI: 10.1139/y98-025     Document Type: Article

References (52)

1. Akasu, T., Shinnick-Gallagher, P., and Gallagher, J.P. 1984. Adenosine mediates a slow hyperpolarizing synaptic potential in autonomic neurones. Nature (London), 311: 62-65.
2. Alexander, S.P.H., and Peters, J.A. 1997. Receptor and ion channel nomenclature supplement. Trends Pharmacol. Sci. Suppl. 8: 1-84.
3. Bayliss, D.A., Viana, F., Bellingham, M.C., and Berger, A.J. 1994. Characteristics and postnatal development of a hyperpolarization-activated inward current in rat hypoglossal motoneurones in vitro. J. Neurophysiol. 71: 119-128.
4. Bergey, G.K., Fitzgerald, S.C., Schrier, B.K., and Nelson, P.G. 1981. Neuronal maturation in mammalian cell culture is dependent on spontaneous electrical activity. Brain Res. 207: 49-58.
5. Bottenstein, J.E., and Sato, G.H. 1979. Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc. Natl. Acad. Sci. U.S.A. 76: 514-517.
6. Bracci, E., Ballerini, L., and Nistri, A. 1996. Spontaneous rhythmic bursts induced by pharmacological block of inhibition in lumbar motoneurones of the neonatal rat spinal cord. J. Neurophysiol. 75: 640-647.
7. Brown, D.A., Caulfield, M.P., and Kirby, P.J. 1979. Relation between catecholamine-induced cyclic AMP changes and hyperpolarization in isolated rat sympathetic ganglia. J. Physiol. (London), 290: 441-451.
8. Burnstock, G., and Wood, J.N. 1996. Purinergic receptors: their role in nociception and primary afferent neurotransmission. Curr. Opin. Neurobiol. 6: 526-532.
9. Crick, H., and Wallis, D.I. 1991. Inhibition of reflex responses of neonate rat lumbar spinal cord by 5-hydroxytryptamine. Br. J. Pharmacol. 103: 1769-1775.
10. Delander, G.E., and Wahl, J.J. 1988. Behaviour induced by putative nociceptive neurotransmitters is inhibited by adenosine or adenosine analogues coadministered intrathecally. J. Pharmacol. Exp. Ther. 246: 565-570.
11. Elliott, P., Foster, G.A., Stringer, B.M.J., and Wallis, D.I. 1995. Characterization of purified populations of cultured rat motoneurones. Brain Res. Assoc. Abstr. 12: 76.
12. Elliott, P., Foster, G.A., Stringer, B.M.J., and Wallis, D.I. 1996. Glutamatergic and glycinergic postsynaptic potentials in primary cultures of rat motoneurones. Br. J. Pharmacol. 118: 138P.
13. Fagg, G.D., and Matus, A. 1984. Selective association of N-methyl aspartate and quisqualate types of L-glutamate receptor with brain postsynaptic densities. Proc. Natl. Acad. Sci. U.S.A. 81: 6876-6880.
14. Fredholm, B.B., Abbracchio, M.P., Burnstock, G., Daly, J.W., Harden, T.K., Jacobson, K.A., Leff, P., and Williams, M. 1994. Nomenclature and classification of purinoceptors. Pharmacol. Rev. 46: 143-156.
15. Fulton, B.P., and Walton, W.D. 1986. Electrophysiological properties of neonatal rat motoneurones studied in vitro. J. Physiol. (London), 370: 657-678.
16. Fuortes, M.G.F., and Nelson, P.G. 1963. Strychnine: its action on spinal motoneurones of cat. Science (Washington, D.C.), 140: 806-808.
17. Grillner, S., Wallen, P., and Brodin, L. 1991. Neuronal network generating locomotor behaviour in the lamprey. Annu. Rev. Neurosci. 14: 169-199.
18. Hamill, O.P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F.J. 1981. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pfluegers Arch. 391: 85-100.
19. Henderson, C.E., Phillips, H.S., Pollock, R.A., Davies, A.M., Lemeulle, C., Armanini, M., Simpson, L.C., Moffet, B., Vandlen, R.A., Koliatsos, V.E., and Rosenthal, A. 1994. GDNF: a potent survival factor for motoneurones present in peripheral nerve and muscle. Science (Washington, D.C.), 266: 1062-1064.
20. Heyer, E.J., Nowak, L.M., and MacDonald, R.L. 1981. Bicuculline: a convulsant with synaptic and nonsynaptic actions. Neurology, 31: 1381-1390.
21. Juurlink, B.H., Munoz, D., and Devon, R. 1990. Calcitonin gene-related peptide identifies spinal motoneurones in vitro. J. Neurosci. Res. 26: 238-241.
22. Kalb, R.G., and Hockfield, S. 1990. Large diameter primary afferent input is required for expression of the Cat-301 proteoglycan on the surface of motor neurones. Neuroscience, 34: 391-401.
23. King, B.F., Wildman, S.S., and Burnstock, G. 1997. Antagonism of a novel Pl receptor in follicular Xenopus oocytes. Br. J. Pharmacol. 120: 132P.
24. Legendre, P., McKenzie, J.S., Dupouy, B., and Vincent, J.D. 1985. Evidence for bursting pacemaker neurones in cultured spinal cord cells. Neuroscience, 16: 753-767.
25. MacDonald, R.L., and Barker, J.L. 1978. Specific antagonism of GABA-mediated postsynaptic inhibition in cultured spinal cord neurons: a common mode of convulsant action. Neurology, 28: 325-330.
26. Malcangio, M., and Bowery, N.G. 1996. GABA receptors in the spinal cord. Trends Pharmacol. Sci. 17: 457-462.
27. Mayer, M.L., and Westbrook, G.L. 1984. Mixed agonist action of excitatory amino acids on mouse spinal cord neurones under voltage clamp. J. Physiol. (London), 354: 29-53.
28. Mayer, M.L., and Westbrook, G.L. 1985. The action of N-methyl-D-aspartic acid on mouse spinal neurones in culture. J. Physiol. (London), 361: 65-90.
29. Navarette, R., and Vrbová, G. 1983. Changes of activity patterns in slow and fast muscles during postnatal development. Dev. Brain Res. 8: 11-19.
30. Neher, E. 1992. Correction for liquid junction potentials in patch-clamp experiments. In Methods in enzymology. Vol. 207. Ion channels. Edited by B. Ruby and L.E. Iverson. Academic Press, London and New York. pp. 123-130.
31. Nicola, M.A., Becker, C.-M., and Triller, A. 1992. Development of glycine receptor alpha subunit in cultivated rat spinal neurones: an immunocytochemical study. Neurosci. Lett. 138: 173-178.
32. O'Brien, R.J., and Fischbach, G.D. 1986a. Isolation of embyronic chick motoneurons and their survival in vitro. J. Neurosci. 6: 3265-3274.
33. O'Brien, R.J., and Fischbach, G.D. 1986b. haracterization of excitatory amino acid receptors expressed by embryonic chick motoneurons in vitro. J. Neurosci. 6: 3275-3283.
34. O'Brien, R.J., and Fischbach, G.D. 1986c. Excitatory synaptic transmission between interneurons and motoneurons in chick spinal cord cell cultures. J. Neurosci. 6: 3284-3289.
35. Obrietan, K., Belousov, A.B., Heller, H.C., and van den Pol, A.N. 1995. Adenosine pre-and postsynaptic modulation of glutamate-dependent calcium activity in hypothalamic neurones. J. Neurophysiol. 74: 2150-2162.
36. Pin, J.P., and Duvoisin, R. 1995. The metabotropic glutamate receptors: structure and functions. Neuropharmacology, 34: 1-26.
37. Rossignol, S., and Dubuc, R. 1994. Spinal pattern generation. Curr. Opin. Neurobiol. 4: 894-902.
38. + channels in the somata of rat motoneurones. J. Physiol. (London), 487: 91-106.
39. Sawynok, J., Reid, A., and Isbrucker, R. 1990. Adenosine mediates calcium-induced antinociception and potentiation of noradrenergic antinociception in the spinal cord. Brain Res. 524: 187-195.
40. Schnaar, R.L., and Schaffner, A.E. 1981. Separation of cell types from embryonic chicken and rat spinal cord: characterization of motoneuron-enriched fractions. J. Neurosci. 1: 203-217.
41. Schwindt, P.C., and Crill, W.E. 1981. Voltage clamp study of cat spinal motoneurons during strychnine-induced seizures. Brain Res. 204: 226-230.
42. Shupliakov, O., Ornung, G., Brodin, L., Ulfhake, B., Ottersen, O.P., Storm-Mathisen, J., and Cullheim, S. 1993. Immunocytochemical localization of amino acid neurotransmitter candidates in the ventral horn of the cat spinal cord: a light microscopic study. Exp. Brain Res. 96: 404-418.
43. Spenger, C., Braschler, U.F., Streit, J., and Lüscher, H.-R. 1991. An organotypic spinal cord - dorsal root ganglion - skeletal muscle coculture of embryonic rat. I. The morphological correlates of the spinal reflex arc. Eur. J. Neurosci. 3: 1037-1053.
44. Streit, J. 1993. Regular oscillation of synaptic activity in spinal networks in vitro. J. Neurophysiol. 70: 871-878.
45. Takahashi, T. 1990. Membrane currents in visually identified motoneurones in neonatal rat spinal cord. J. Physiol. (London), 423: 27-46.
46. Viana, F., Bayliss, D.A., and Berger, A.J. 1994. Postnatal changes in rat hypoglossal motoneurone membrane properties. Neuroscience, 59: 131-148.
47. 1 receptors attenuates synaptic transmission to immature rat motoneurones in vitro. Brain Res. 554: 111-121.
48. Wu, W.-L., Ziskind-Conhaim, L., and Sweet, M.A. 1992. Early development of glycine-and GABA-mediated synapses in rat spinal cord. J. Neurosci. 12: 3935-3945.
49. Yan, Q., Matheson, C., and Lopez, O.T. 1995. In vivo neurotophic effects of GDNF on neonatal and adult facial motor neurons. Nature, 373: 341-344.
50. Zeman, S., and Lodge, D. 1992. Pharmacological characterization of non-NMDA subtypes of glutamate receptor in the neonatal rat hemisected spinal cord in vitro. Br. J. Pharmacol. 106: 367-372.
51. Ziskind-Conhaim, L. 1988. Electrical properties of motoneurones in the spinal cord of rat embryos. Dev. Biol. 128: 21-29.
52. Zorumski, C.F., and Yang, J. 1988. AMPA, kainate and quisqualate activate a common receptor-channel complex on embryonic motoneurons. J. Neurosci. 8: 4277-4286.