Converting trypsin to elastase: Substitution of the S1 site and adjacent loops reconstitutes esterase specificity but not amidase activity

Protein Engineering

Volumn 11, Issue 8, 1998, Pages 669-673

  Hung, Su Hwi ^a,b   Hedstrom, Lizbeth ^b  

^a UNIVERSITY OF FLORIDA   (United States)

^b BRANDEIS UNIVERSITY   (United States)

Author keywords

Serine protease; Substrate specificity

Indexed keywords

AMIDASE; ELASTASE; MUTANT PROTEIN; TRYPSIN;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE;

FELIS CATUS;

EID: 0031813817     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/11.8.669     Document Type: Article

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