Clostridium difficile toxin B induces apoptosis in intestinal cultured cells

Infection and Immunity

Volumn 66, Issue 6, 1998, Pages 2660-2665

  Fiorentini, Carla ^a   Fabbri, Alessia ^a   Falzano, Loredana ^a   Fattorossi, Andrea ^a   Matarrese, Paola ^a   Rivabene, Roberto ^a   Donelli, Gianfranco ^a  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN B;

ANIMAL CELL; APOPTOSIS; ARTICLE; CHROMATIN CONDENSATION; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; DIARRHEA; FLOW CYTOMETRY; HUMAN; HUMAN CELL; INTESTINE CELL; NONHUMAN; PRIORITY JOURNAL; PSEUDOMEMBRANOUS COLITIS;

CLOSTRIDIUM DIFFICILE;

EID: 0031813370     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.66.6.2660-2665.1998     Document Type: Article

References (37)

1. Aullo, P., M. Giry, M. R. Popoff, C. Kocks, and P. Hoquet. 1993. A chimeric toxin to study the role of the 21 kDa GTP binding protein rho in the control of actin microfilament assembly. EMBO J. 12:921-931.
2. Chaves-Olarte, E., I. Florin, P. Boquet, M. Popoff, C. von Eichel-Streiber, and M. Thelestam. 1996. UDP-glucose deficiency in a mutant cell line protects again alucosyltransferase toxins from Clostridium difficile and Clostridium sordellii. J. Biol. Chem. 271:6925-6932.
3. Darzynkiewicz, Z., G. Juan, X. Li, W. Gorczyca, T. Murakami, and F. Traganos. 1997. Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry 27:1-20.
4. Earnshaw, W. C. 1995. Apoptosis: lessons from in vitro systems. Trends Cell Biol. 5:217-220.
5. Ferlini, C., S. Di Cesare, G. Rainaldi, W. Malorni, P. Samoggia, R. Bise, and A. Fattorossi. 1996. A flow cytometric analysis of the early phases of apoptosis by cellular and nuclear techniques. Cytometry 24:106-115.
6. Fiorentini, C., G. Arancia, S. Paradisi, G. Donelli, M. Giuliano, F. Piemonte, and P. Mastrantonio. 1989. Effects of Clostridium difficile toxins A and B on cytoskeleton organization in HEp-2 cells: a comparative morphological study. Toxicon 27:1209-1218.
7. 2+-independent cytotoxic effects in cultured normal rat intestinal crypt cells. Infect. Immun. 61:3988-3993.
8. Fiorentini, C., A. Fabbri, G. Flatau, G. Donelli, P. Matarrese, E. Lemichez, L. Falzano, and P. Boquet. 1997. Escherichia coli cytotoxic necrotizing factor 1 (CNF1): a toxin which activates the Rho GTPase. J. Biol. Chem. 272: 19532-19537.
9. Fiorentini, C., A. Fabbri, P. Matarrese, L. Falzano, P. Boquet, and W. Malorni. 1997. Hinderance of apoptosis and phagocytic behaviour induced by E. coli necrotizing factor 1 (CNF1): two related activities in epithelial cells. Biochem. Biophys. Res. Commun. 241:341-346.
10. Fiorentini, C., P. Matarrese, E. Straface, L. Falzano, A. Fabbri, G. Donelli, A. Cossarizza, P. Boquet, and W. Malorni. Toxin-induced activation of Rho GTP-binding protein increases Bcl-2 expression and influences mitochondrial homeostasis. Exp. Cell Res., in press.
11. Flatau, G., E. Lemichez, M. Gauthier, P. Chardin, S. Paris, C. Fiorentini, and P. Boquet. 1997. Toxin-induced activation of the G protein p21 Rho by deamidation of glutamine. Nature 387:729-733.
12. Gamen, S., I. Marzo, A. Anel, A. Pineiro, and J. Naval. 1996. CPP32 inhibition prevents Fas-induced ceramide generation and apoptosis in human cells. FEBS Lett. 390:233-237.
13. Hall, A. 1998. Rho GTPases and the actin cytoskeleton. Science 279:509-514.
14. Henning, S. W., R. Galandrini, A. Hall, and D. A. Cantrell. 1997. The GTPase Rho has a critical regulatory role in thymus development. EMBO J. 16:2397-2407.
15. Just, I., J. Selzer, M. Wilm, C. von Eichel-Streiber, M. Mann, and K. Aktories. 1995. Glycosylation of rho proteins by Clostridium difficile toxin B. Nature (London) 375:500-503.
16. Just, I., M. Selzer, J. Rex, C. von Eichel-Streiber. M. Mann, and K. Aktories. 1995. The enterotoxin from Clostridium difficile (ToxA) monoglucosylates the Rho protein. J. Biol. Chem. 270:13392-13396.
17. Khelef, N., A. Zychlinsky, and N. Guiso. 1993. Bordetella pertussis induces apoptosis in macrophages: role of adenylate cyclase-hemolysin. Infect. Immun. 61:4064-4071.
18. Kramer, G., P. Petit, Z. Naoufal, L.-L. Vayssière, and B. Mignotte. 1995 The biochemistry of programmed cell death. FASEB J. 9:1277-1287.
19. Mahida, Y. R., S. Makh, S. Hyde, T. Gray, and S. P. Borriello. 1996. Effect of Clostridium difficile toxin A on human intestinal epithelial cells: induction of interleukin 8 production and apoptosis after cell detachment. Gut 38: 337-347.
20. Malorni, W., C. Fiorentini, S. Paradisi, M, Giuliano, P. Mastrantonio, and G. Donelli. 1990. Surface blebbing and cytoskeletal changes induced in vitro by toxin B from Clostridium difficile: an immunochemical and ultrastructural study. Exp. Mol. Pathol. 52:340-356.
21. Martins, L. M., and W. Earnshaw. 1997. Apoptosis: alive and kicking in 1997. Trends Cell Biol. 7:111-114.
22. Mastrantonio, P., A. Pantosti, M. Cerquetti, C. Fiorentini, and G. Donelli. 1996. Clostridium difficile, an update on virulence mechanisms. Anaerobe 2: 337-343.
23. Meredith, J. E., Jr., and M. A. Sehwartz. 1997. Integrins, adhesion and apoptosis. Trends Cell Biol. 7:146-150.
24. Moorman, J. P., D. A. Bobak, and C. S. Hahn. 1996. Inactivation of the small GTP binding protein Rho induces multinucleate cell formation and apoptosis in murine T lymphoma EL4. J. Immunol. 156:4146-4153.
25. Na, S., T. H. Chuang, A. Cunningham, T. G. Turi, J. H. Hanke, G. M. Bokoch, and D. E. Danley. 1996. D4-GDI, a substrate of CPP32, is proteolyzed durine Fas-induced apoptosis. J. Biol. Chem. 271:11209-11213.
26. Olson, M. F., A. Ashworth, and A. Hall. 1995. An essential role for Rho, Rac and CDC42 GTPases in cell cycle progression through G1. Science 269: 1270-1272.
27. Orrenius, S. 1995. Apoptosis: molecular mechanisms and implications for human disease. J. Int. Med. 237:529-536.
28. Popoff, M. R., E. Chaves-Olarte, E. Lemichez, C. von Eichel-Slreiber, M. Thelestam, P. Chardin, D. Cussac, B. Antonny, P. Chavrier, G. Flatau, M. Giry, J. Gunzburg, and P. Boquet. 1996. Ras, Rap, and Rac small GTP-binding proteins are targets for Clostridium sordellii lethal toxin glucosylation. J. Biol. Chem. 271:10217-10224.
29. Popoff, M. R., F. W. Milward, B. Bancillon, and P. Boquet. 1989. Purification of the Clostridium spiroforme binary toxin and activity of the toxin on HEp-2 cells. Infect. Immun. 57:2462-2469.
30. Potten, C. S. 1997. Epithelial cell growth and differentiation. II. Intestinal apoptosis. Am. J. Physiol. 273:G253-G257.
31. Richter, C., M. Schweizer, A. Cossarizza, and C. Franceschi. 1996. Control of apoptosis by the cellular ATP level. FEBS Lett. 378:107-110.
32. Ruoslahti, E. 1997. Stretching is good for a cell. Science 276:1345-1346.
33. Thelestam, M., I. Florin, and E. C. Olarte. 1997. Review: Clostridium difficile toxins, p. 141-158. In K. Aklories (ed.), Bacterial toxins: a laboratory companion. Chapman & Hall, Ltd. London, England.
34. von Eichel Streiber, C., P. Boquet, M. Sauerborn, and M. Thelestam. 1997. Large clostridial cytotoxins - a family of glycosyltransferases modifying small GTP-binding proteins. Trends Microbiol. 4:375-382.
35. von Eichel Streiber, C., U. Harperath, D. Bosse, and U. Hadding. 1987. Purification of two high molecular weight toxins of Clostridium difficile which are antigenically related. Microb. Pathog. 2:307-318.
36. Zychlinsky, A., and P. Sansonetti. 1997. Apoposis in bacterial palhogenesis. J. Clin. Invest. 100:493-496.
37. Zychlinsky, A., C. Fitting, J. M. Cavaillon, and P. J. Sansonetti. 1994. Interleukin 1 is released by murine macrophages during apoptosis induced by Shigella flexneri. J. Clin. Invest. 94:1328-1332.