Use of liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) for routine identification of enzymatically digested proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Electrophoresis

Volumn 19, Issue 6, 1998, Pages 1046-1052

  Stone, Kathryn L ^a   DeAngelis, Raymond ^a   LoPresti, Mary ^a   Jones, Joshua ^a   Papov, Vladimir V ^a   Williams, Kenneth R ^a  

^a YALE UNIVERSITY   (United States)

Author keywords

In gel digestion; Mass spectrometry; Protein identification; Sequest; Sodium dodecyl sulfate polyacrylamide gel electrophoresis

Indexed keywords

ELECTROPHORESIS; ELECTROSPRAY IONIZATION; KERATIN; LIQUID CHROMATOGRAPHY; MASS SPECTROMETERS; SEARCH ENGINES; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

ELECTROSPRAY IONIZATION TANDEM MASS SPECTROMETRY; INGEL DIGESTION; LC-ESI-MS/MS; LC/ESI-MS/MS; LIQUID CHROMATOGRAPHY ELECTROSPRAY; MS/MS ANALYSIS; PROTEIN IDENTIFICATION; SEQUEST; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; TRYPTIC DIGESTS;

MASS SPECTROMETRY;

KERATIN; PROTEINASE;

ANALYTICAL EQUIPMENT; ANALYTICAL PARAMETERS; ARTICLE; AUTOANALYZER; EXPERIMENTAL TEST; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LABORATORY; MASS SPECTROMETRY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN PURIFICATION;

EID: 0031812223     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150190620     Document Type: Article

References (6)

1. Science 1997, 277, 1433.
2. Eng, J. K., McCormack, A. L., Yates, J. R., Am. Soc. Mass Spectrom. 1994, 5, 976-989.
3. Yates, J. R. , Eng, J. K., McCormack, A. L., Anal. Chem. 1995, 67, 3202-3210.
4. Stone, K. L., Williams, K. R., Current Protocols in Protein Science, John Wiley, New York 1995, pp. 11.3.1-11.3.13.
5. Roepstorff, P., Moller, J., Biomed Mass Spectrom. 1984, 11, 601.
6. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., Lipman, D. J., J. Mol. Biol. 215, 403-410.