DNA extraction from small blood volumes and the processing of cellulose blood cards for use in polymerase chain reaction

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 9, Issue 1, 1998, Pages 1-5

  Mackey, Karol ^a   Steinkamp, Amy ^a   Chomczynski, Piotr ^b  

^a Molecular Research Center   (United States)

^b UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

Blood; Blood cards; DNA; DNA extraction; Guthrie cards; Polymerase chain reaction, PCR; Small blood volume

Indexed keywords

CELLULOSE; DNA;

ARTICLE; BLOOD; BLOOD SAMPLING; BLOOD STORAGE; BLOOD VOLUME; DNA DETERMINATION; GENE AMPLIFICATION; GENOME; HUMAN; POLYMERASE CHAIN REACTION;

EID: 0031812081     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02752692     Document Type: Article

References (7)

1. Mackey, K., Williams, P., Seim, S., and Chomczynski, P. (1996) The use of DNAzol for the rapid isolation of genomic DNA from whole blood. Biomed. Prod. (Suppl.) 13-15.
2. ®: a reagent for the rapid isolation of genomic DNA. BioTechniques 22, 550-553.
3. Cox, R. (1968) The use of guanidium chloride in the isolation of nucleic acids. Methods Enzymol 12(B), 120-129.
4. Chirgwin, J., Przybyla, R., MacDoinald, R. and Rutter, W. (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18, 5294-5299.
5. McCabe, E., Huang, S., Seltzer, W., and Law, M. (1987) DNA microextraction from dried blood spots on filter paper blotters: potential applications to newborn screening. Human Genet. 75, 213-216.
6. Carducci, C., Ellul, L., Antonozzi, I., and Pontecorvi, A. (1992) DNA elution and amplification by polymerase chain reaction from dried blood spots. BioTechniques 13, 735-737.
7. Jinks, D., Minter, M., Tarver, D., Vanderford, M., Hejtmancik, J., and McCabe, E. (1989) Molecular genetic diagnosis of sickle cell disease using dried blood specimens on blotters used for newborn screening. Human Genet. 81, 363-366.