Methotrexate induces apoptotic cell death in human keratinocytes

Archives of Dermatological Research

Volumn 290, Issue 5, 1998, Pages 240-245

  Heenen, Michel ^a   Laporte, Marianne ^a   Noel, Jean Christophe ^a   De Graef, Chantal ^a  

^a ERASME HOSPITAL   (Belgium)

Author keywords

Apoptotic cell death; Keratinocyte culture; Methotrexate; Psoriasis

Indexed keywords

DNA FRAGMENT; METHOTREXATE; PROTEIN P53;

APOPTOSIS; ARTICLE; CELL CULTURE; CELL DAMAGE; CELL DEATH; CONTROLLED STUDY; DERMIS; DRUG MECHANISM; ELECTRON MICROSCOPY; EPIDERMIS; EXPLANT; HISTOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; KERATINOCYTE; MORPHOLOGY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PSORIASIS;

APOPTOSIS; CELL COUNT; CELL NUCLEUS; CELLS, CULTURED; DERMATOLOGIC AGENTS; DNA FRAGMENTATION; HUMANS; KERATINOCYTES; METHOTREXATE; PSORIASIS; SKIN; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0031811423     PISSN: 03403696     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004030050298     Document Type: Article

References (24)

1. Olsen EA (1991) The pharmacology of methotrexate. J Am Acad Dermatol 25:301-318
2. Weinstein GD, Valasco J (1972) Selective action of methotrexate on psoriatic epidermal cells. J Invest Dermatol 59:121-127
3. Kamel R, Al-Hakim H, Rademaker M, Thomas R (1988) Pharmacokinetics of small doses of methotrexate in patients with psoriasis. Acta Derm Venereol (Stockh) 68:267-270
4. Weinstein G, Goldfaden G, Frost P (1971) Methotrexate: mechanism of action on DNA synthesis in psoriasis. Arch Dermatol 104:236-243
5. Wyllie AH, Kerr JFR, Currie AR (1980) Cell death: the significance of apoptosis. Int Rev Cytol 68:251-306
6. Regnier M, Schweizer J, Michel S, Bailly C, Prunieras M (1986) Expression of high molecular weight (67 K) keratin in human keratinocytes cultured on dead deepidermized dermis. Exp Cell Res 165:63-67
7. Heenen M, De Graef CH, Parent D, De Dobbeleer G, Galand P (1992) Renewal and differentiation of keratinocytes cultured on dead de-epidermalized dermis. Cell Prolif 25:311-319
8. Lee JM, Bernstein A (1995) Apoptosis, cancer and the p53 tumor suppression gene. Cancer Metastasis Rev 14:149-161
9. Urano Y, Asano T, Yoshimoto K (1995) Frequent p53 accumulation in the chronically sun exposed epidermis and clonal expansion of p53 mutant cells in the epidermis adjacent to basal cell carcinoma. J Invest Dermatol 104:928-932
10. Laporte M, Galand P, Fokan D, Degraef CH, Heenen M (1996) Apoptosis in normal skin, psoriasis and healing psoriasis. J Invest Dermatol 106:865
11. Newton JA, Camplejohn RS, Bowyer C, McGibbon DH, Wright NA (1985) Study of psoriatic epidermal cell kinetics and cell death after oral methotrexate. Dermatologica 171:469-473
12. Gavrieli Y, Sherman Y, Ben-Sasson S (1992) Identification of programmed cell death in situ via specific labelling of nuclear fragmentation. J Cell Biol 119:493-501
13. Majno G, Joris I (1995) Apoptosis, oncosis and necrosis: an overview of cell death. Am J Pathol 146:3-15
14. Schwanz SM, Bennett MR (1995) Death by any other name. Am J Pathol 147:229-234
15. James SJ, Basnakian AG, Miller BJ (1994) In vitro folate deficiency induces deoxynucleotide pool imbalance, apoptosis and mutagenesis in Chinese hamster ovary cells. Cancer Res 54: 5075-5080
16. Golos B, Malic J (1989) Enhancement of methotrexate induced growth inhibition, cell killing and DNA lesions in cultured L5178 cells by the reduction of DNA repair efficiency. Biochem Pharmacol 38:1743-1748
17. Snyder RD (1988) Consequences of the depletion of cellular deoxynucleoside triphosphate pools on the excision-repair process in cultured human fibroblasts. Mutat Res 200: 193-199
18. Hall PA, McKee PH, Du P, Menage H, Robin D, Lane PA (1993) High level of p53 protein in UV irradiated normal human skin. Oncogene 8:203-207
19. Schwartz PM, Barnett SK, Reuveni H (1991) Thymidine salvage changes with differentiation in human keratinocytes in vitro. J Invest Dermatol 97:1057-1060
20. Jeffes EWB III, McCullough JL, Pittelkow MR, McCormick A, Almanzor J, Liu G, Dang M, Voss K, Schlotzhauer A, Weinstein GD (1995) Methotrexate therapy of psoriasis: differential sensitivity of proliferating cells to the cytotoxic and growth-inhibitory effects of methotrexate. J Invest Dermatol 104:183-188
21. Schwartz PM, Barnett SK, Atillasoy ES, Milstone LM (1992) Methotrexate induces differentiation of human keratinocytes. Proc Natl Acad Sci U S A 89:594-598
22. Linser I, Auböck J, Romain N, Smolle J, Grubauer G, Fritsch P, Hintner H (1987) The keratin body phenomenon. Epitelia 1:85-99
23. Sepp N, Linser I, Hintner H, Fritsch P (1990) Disturbed epidermal homeostasis in psoriasis. J Invest Dermatol 95:488
24. Heenen M, Galand P, De Maertelaere V, Heenen PH (1987) Psoriasis: hyperproliferation cannot induce characteristic epidermal morphology. Cell Tissue Kinet 20:561-570