Smooth muscle actin and myosin expression in cultured airway smooth muscle cells

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 274, Issue 5 18-5, 1998, Pages

  Wong, Jean Z ^a   Woodcock Mitchell, Janet ^a   Mitchell, John ^a   Rippetoe, Patricia ^a   White, Sheryl ^a   Absher, Marlene ^a   Baldor, Linda ^a   Evans, John ^a   McHugh, Kirk M ^b   Low, Robert B ^a  

^a UNIVERSITY OF VERMONT   (United States)

^b THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

Growth state

Indexed keywords

ACTIN; CONTRACTILE PROTEIN; MYOSIN;

AIRWAY PRESSURE; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CELL GROWTH; GENE EXPRESSION; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RAT; RNA ANALYSIS; SMOOTH MUSCLE;

EID: 0031808859     PISSN: 10400605     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajplung.1998.274.5.l786     Document Type: Article

References (35)

1. Absher, M., W. Makrides, P. Shapiro, and J. N. Evans. Hyperoxia inhibits proliferation of cultured rat tracheal smooth muscle cells. Am. J. Physiol. 267 (Lung Cell. Mol. Physiol. 11): L101-L105, 1994.
2. Agrawal, D. K., and R. G. Townley. Airway Smooth Muscle: Modulation of Receptors and Response. Boca Raton, FL: CRC, 1990.
3. Babij, P., S. Kawamoto, S. White, R. Adelstein, and M. Periasamy. Differential expression of smooth muscle myosin heavy chain isoform SM1 and SM2 in cultured vascular smooth muscle cells. Am. J. Physiol. 262 (Cell Physiol. 31): C607-C613, 1992.
4. Babij, P., and M. Periasamy. Myosin heavy chain isoform diversity in smooth muscle is produced by differential RNA processing. J. Mol. Biol. 210: 673-679, 1989.
5. Babij, P., J. Zhao, S. White, J. Woodcock-Mitchell, J. Mitchell, M. Absher, L. Baldor, M. Periasamy, and R. B. Low. Smooth muscle myosin regulation by serum and cell density in cultured rat lung mesenchymal cells. Am. J. Physiol. 265 (Lung Cell. Mol. Physiol. 9): L127-L132, 1993.
6. Blank, R. S., M. M. Thompson, and G. Owens. Cell cycle versus density dependence of smooth muscle alpha actin expression in cultured rat aotic smooth muscle cells. J. Cell Biol. 107: 299-306, 1988.
7. Chomczynski, P., and N. Sacchi. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156-159, 1987.
8. Corjay, M. H., M. M. Thompson, K. R. Lynch, and G. K. Owens. Differential effect of platelet-derived growth factor versus serum-induced growth on smooth muscle alpha actin and nonmuscle beta-actin expression in cultured rat aortic smooth muscle cells. J. Biol. Chem. 264: 10501-10506, 1989.
9. Desmoulieres, A., and G. Gabbiani. Smooth muscle cell and fibroblast biological and functional features. In: The Vascular Smooth Muscle Cell: Molecular and Biological Responses to the Extracellular Matrix, edited by S. M. Schwartz and R. P. Mecham. New York: Academic, 1995, p. 329-359.
10. Garfinkel, L. I., M. Periasamy, and B. Nadal-Ginard. Cloning and characterization of DNA sequences corresponding to myosin light chains 1,2 and 3, troponin-C, troponin-T, α-tropomyosin and α-actin. J. Biol. Chem. 257: 11078-11086, 1982.
11. Halayko, A. J., H. Salari, X. Ma, and N. L. Stephens. Markers of airway smooth muscle cell phenotype. Am. J. Physiol. 270 (Lung Cell. Mol. Physiol. 14): L1040-L1051, 1996.
12. Hershenson, M. B., S. Aghilli, N. Punjabi, C. Hernandez, D. W. Ray, A. Garland, S. Glagov, and J. Solway. Hyperoxia-induced airway hyperresponsiveness and remodeling in immature rats. Am. J. Physiol. 262 (Lung Cell. Mol. Physiol. 6): L263-L269, 1992.
13. 3H]thymidine pulse-labeling of intimal, medial, and adventitial cells in microvessels and at the hilum. Am. Rev. Respir. Dis. 140: 1471-1477, 1989.
14. Kuro-o, M., R. Nagai, K. Nakahara, R. Tsai, H. Tsuchimochi, Y. Yazaki, A. Ohkubo, and F. Takaku. cDNA cloning of a myosin heavy chain isoform in embryonic smooth muscle and its expression during vascular development and in arteriosclerosis. J. Biol. Chem. 266: 3768-3773, 1991.
15. Kuro-o, M., R. Nagai, H. Tsuchimochi, H. Katoh, Y. Yazaki, A. Ohkubo, and F. Takaku. Developmentally regulated expression of vascular smooth muscle myosin heavy chain isoforms. J. Biol. Chem. 264: 18272-18275, 1989.
16. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage. Nature 227: 680-685, 1970.
17. Larsen, D. M., K. Fujiwara, R. W. Alexander, and M. A. Gimbrone. Myosin in cultured vascular smooth muscle cells: immunofluorescence and immunochemical studies of alterations in antigenic expression. J. Cell Biol. 99: 1582-1589, 1984.
18. Leslie, K., T. King, and R. Low. Smooth muscle actin is expressed by air space fibroblast-like cells in idiopathic pulmonary fibrosis and hypersensitivity pneumonitis. Chest 99: 47S-48S, 1991.
19. Maniatis, T., E. F. Frotsch, and J. Sambrook. Molecular Cloning. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, 1982.
20. McHugh, K. M., K. Crawford, and J. L. Lessard. A comprehensive analysis of the developmental and tissue-specific expression of the isoactin multigene family in the rat. Dev. Biol. 148: 442-458, 1991.
21. McHugh, K. M., and J. L. Lessard. The development expression of the rat α-vascular and γ-enteric smooth muscle isoactins: isolation and characterization of a rat γ-enteric actin cDNA. Mol. Cell. Biol. 8: 5223-5231, 1988.
22. Mitchell, J. J., J. L. Woodcock-Mitchell, L. Perry, J. Zhao, R. B. Low, L. Baldor, and P. M. Absher. In vitro expression of the α-smooth muscle actin isoform by rat lung mesenchymal cells: regulation by culture condition and TGFβ. Am. J. Respir. Cell Mol. Biol. 9: 10-18, 1993.
23. Mitchell, J. J., J. L. Woodcock-Mitchell, S. Reynolds, R. B. Low, K. Leslie, K. Adler, G. Gabbiani, and O. Skalli. α-Smooth muscle actin in parenchymal cells of bleomycin-injured rat lung. Lab. Invest. 60: 643-650, 1989.
24. Moffatt, R. K., D. M. Hyde, C. G. Plopper, W. S. Tyler, and L. F. Putney. Ozone-induced adaptive and reactive cellular changes in respiratory bronchioles of Bonnet monkeys. Exp. Lung Res. 12: 57-74, 1987.
25. O'Farrell, P. H. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007-4021, 1975.
26. Owens, G. K. Regulation of differentiation of vascular smooth muscle cells. Physiol. Rev. 75: 487-517, 1995.
27. Panettieri, R. Airways smooth muscle cell growth and proliferation. In: Airways Smooth Muscle: Development and Regulation of Contractility, edited by D. Raeburn and M. Giembycz. Basel, Switzerland: Birkhausen Verlag, 1994, p. 41-68.
28. Panettieri, R. A., P. A. Yadvish, A. M. Kelly, N. A. Rubenstein, and M. I. Kotlikoff. Histamine stimulates proliferation of airway smooth muscle and induces c-fos expression. Am. J. Physiol. 259 (Lung Cell. Mol. Physiol. 3): L365-L371, 1990.
29. Rippetoe, P. E., J. Woodcock-Mitchell, J. Mitchell, R. B. Low, and J. N. Evans. Hyperoxia induces changes in expression of lung smooth muscle contractile proteins (Abstract). Am. Rev. Respir. Dis. 145: A389, 1992.
30. Rovner, A. S., R. A. Murphy, and G. K. Owens. Expression of smooth muscle and nonmuscle myosin heavy chains in cultured vascular smooth muscle cells. J. Biol. Chem. 261: 14740-14745, 1986.
31. Schwartz, S. M., and R. P. Mecham. The Vascular Smooth Muscle Cell: Molecular and Biological Responses to the Extracellular Matrix. New York: Academic, 1995, p. 1-410.
32. Skalli, O. P., A. Ropraz, G. Trzeciak, D. Benzonana, D. Gillessen, and G. Gabbiani. A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103: 2787-2796, 1986.
33. Tsukada, T., D. Tippens, D. Gordon, R. Ross, and A. M. Gown. HHF35, a muscle-actin-specific-monoclonal antibody. I. Immunocytochemcal and biochemical characterization. Am. J. Pathol. 126: 51-56, 1987.
34. Woodcock-Mitchell., J., K. B. Adler, and R. B. Low. Immunohistochemical identification of cell types in normal and in bleomycin-induced fibrotic rat lung (Abstract). Am. Rev. Respir. Dis. 130: 910, 1984.
35. Woodcock-Mitchell, J., S. White, W. Stirewalt, M. Periasamy, J. Mitchell, and R. B. Low. Myosin isoform expression in developing and remodeling rat lung. Am. J. Respir. Cell Mol. Biol. 8: 617-625, 1993.