Differentiation of LA-N-5 neuroblastoma cells into cholinergic neurons: Methods for differentiation, immunohistochemistry and reporter gene introduction

Brain Research Protocols

Volumn 2, Issue 3, 1998, Pages 183-190

  Hill, David P ^a   Robertson, Kent A ^a  

^a INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cholinergic differentiation; Electroporation; Immunohistochemistry; Neuroblastoma; Retinoic acid

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE; RETINOIC ACID;

ARTICLE; CELL DIFFERENTIATION; CELL GROWTH; CELL ULTRASTRUCTURE; CHOLINERGIC NERVE CELL; CONTROLLED STUDY; ELECTROPORATION; GENE EXPRESSION; GROWTH CONE; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; NEUROBLASTOMA CELL; PHEOCHROMOCYTOMA CELL; PRIORITY JOURNAL; REPORTER GENE;

CELL DIFFERENTIATION; CELL DIVISION; GENES, REPORTER; HUMANS; IMMUNOHISTOCHEMISTRY; NEUROBLASTOMA; NEURONS; TRETINOIN; TUMOR CELLS, CULTURED;

EID: 0031808547     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(97)00041-X     Document Type: Article

References (17)

1. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl, Immunohistochemistry, in: K. Janssen, (Ed.), Current Protocols in Molecular Biology, Vol. 2, Wiley and Sons, Boston, MA, 1994, p. 14.6.
2. Breitman T.R., Selonick S.E., Collins S.J. Induction of differentiation of the human promyelocytic leukemia cell line (HL-60) by retinoic acid. Proc. Natl. Acad. Sci. USA. 77:1980;2936-2940.
3. Forrester L.M., Nagy A., Sam M., Watt A., Stevenson L., Bernstein A., Joyner A.L., Wurst W. An induction gene trap screen in embryonic stem cells: identification of genes that respond to retinoic acid in vitro. Proc. Natl. Acad. Sci. USA. 93:1996;1677-1682.
4. Gomez N., Traverse S., Cohen P. Identification of a MAP kinase kinase kinase in phaeochromocytoma (PC12) cells. FEBS Lett. 314:1992;461-465.
5. Han G., Chang B., Connor M.J., Sidell N. Enhanced potency of 9-cis versus all-trans retinoic acid to induce the differentiation of human neuroblastoma cells. Differentiation. 59:1995;61-69.
6. Hill D.P., Robertson K.R. Characterization of the cholinergic neuronal differentiation of the human neuroblastoma cell line LA-N-5 after treatment with retinoic acid. Dev. Brain Res. 102:1997;53-67.
7. Hill D.P., Strome S. Brief cytochalasin-induced disruption of microfilaments during a critical interval in 1-cell C. elegans embryos alters the partitioning of developmental instructions to the 2-cell embryo. Development. 108:1990;159-172.
8. D.P. Hill, W. Wurst, Gene and enhancer trapping: mutagenic strategies for developmental studies, in: R. Pedersen (Ed.), Current Topics in Developmental Biology, Vol. 28, Academic Press, San Diego, CA, 1993, pp. 181-206.
9. D.P. Hill, W. Wurst, The use of gene-trap vectors to identify and mutate developmentally-regulated genes, in: G. Coupland (Ed.), Insertional Mutagenesis: a Practical Approach, Oxford University Press, Oxford, 1997, in press.
10. Keller G.M. In vitro differentiation of embryonic stem cells. Curr. Opin. Cell Biol. 7:1995;862-869.
11. W.D. Linscott, Linscott's Directory of Immunological and Biological Reagents, 9th Edn., Linscott, Santa Rosa, CA, 1996.
12. Robson J.A., Sidell N. Ultrastructural features of a human neuroblastoma cell line treated with retinoic acid. Neuroscience. 14:1985;1149-1162.
13. Shigekawa K., Dower W.J. Electroporation: a general approach to the introduction of macromolecules into prokaryotic and eukaryotic cells. Aust. J. Biotechnol. 3:1989;56-62.
14. Sidell N., Lucas C.A., Kreutzberg G.W. Regulation of acetylcholinesterase activity by retinoic acid in a human neuroblastoma cell line. Exp. Cell Res. 155:1984;305-309.
15. Toneguzzo F., Keating A., Glynn S., McDonald K. Electric field-mediated gene transfer: characterization of DNA transfer and patterns of integration in lymphoid cells. Nucleic Acids Res. 16:1988;5515-5532.
16. Watson J.B., Margulies J.E. Differential cDNA screening strategies to identify novel stage-specific proteins in the developing mammalian brain. Dev. Neurosci. 15:1993;77-86.
17. Wurst W., Rossant J., Prideaux V., Kownacka M., Joyner A., Hill D.P., Guillemot F., Gasca S., Cado D., Auerbach A.et al. A large-scale gene-trap screen for insertional mutations in developmentally regulated genes in mice. Genetics. 139:1995;889-899.