Characterization of galactose-induced extracellular and intracellular pectolytic activities from the exo-1 mutant strain of Neurospora crassa

Journal of Industrial Microbiology and Biotechnology

Volumn 20, Issue 3-4, 1998, Pages 238-243

  Crotti, L B ^a   Terenzi, H F ^a   Jorge, J A ^a   Polizeli, M L T M ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Catabolic repression; Inductor induction; Neurospora crassa; Pectate pectin lyase; Pectic enzymes; Polygalacturonase

Indexed keywords

GALACTOSE; PECTATE LYASE; POLYGALACTURONASE;

ARTICLE; ENZYME INACTIVATION; ENZYME STABILITY; FUNGUS MUTANT; HYDROLYSIS; MOLECULAR WEIGHT; NEUROSPORA CRASSA; NONHUMAN;

FUNGI; NEUROSPORA CRASSA;

EID: 0031807713     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.2900519     Document Type: Article

References (28)

1. Atherton D. 1989. Successful PTC amino acid analysis at the picomole level. In: Techniques in Protein Chemistry (TE Hugli, ed), pp 273-283. Academic Press, New York.
2. Ayers WA, GC Papavizas and AF Diem. 1966. Polygalacturonase trans-eliminase and polygalacturonase production by Rhizoctonia solani. Phytopathology 56: 1006-1011.
3. Bidlingmeyer BA, SA Cohen and TL Tarvin. 1984. Rapid analysis of amino acids using pre-column derivatization. J Chromatogr 336: 93-104.
4. Blum H, H Beier and HJ Gross. 1987. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8: 93-97.
5. Collmer A, JL Ried and MS Mount. 1988. Assay methods for pectic enzymes. In: Methods in Enzymology (Wood WA and ST Kelloggy, eds), Vol 161, pp 326-335, Academic Press, New York.
6. Dubois M, KA Gilles, JK Hamilton, PA Rebers and F Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350-356.
7. Dupont DR, PS Keim, AH Chui, R Bello, MB Bozzini and KJ Wilson. 1989. A comprehensive approach to amino acid analysis. In: Techniques in Protein Chemistry (TE Hugh, ed), pp 284-294, Academic Press, New York.
8. Eberhart BM, RS Beck and KM Goolsby. 1977. Cellulase of Neurospora crassa. J Bacteriol 130: 181-186.
9. Gratzner HG and DN Sheehan. 1969. Neurospora mutant exhibiting hyperproduction of amylase and invertase. J Microbiol 97: 544-549.
10. Guo W, L González-Candelas and PE Kolattukudy. 1995. Cloning of a new pectate lyase gene pelC from Fusarium solani f sp pisi (Nectria haematococca, Mating Type VI) and characterization of the gene product expressed in Pichia pastoris. Arch Biochem Biophys 323: 352-360.
11. Hobohm U, T Houthaeve and C Sander. 1994. Amino acid analysis and protein database compositional search as a rapid and inexpensive method to identify proteins. Anal Biochem 222: 202-209.
12. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
13. Leone G and J Van Den Heuvel. 1987. Regulation by carbohydrates of the sequential 'in vitro' production of pectic enzymes by Botrytis cinerea. J Can Bot 65: 2133-2141.
14. Lester DR, J Speirs, G Orr and CJ Brady. 1994. Peach (Prunus persica) endopolygalacturonase cDNA isolation and mRNA analysis in melting and nonmelting peach cultivars. Plant Physiol 105: 225-231.
15. Manachini PL, MG Fortina and C Parini. 1987. Purification and properties of an endopolygalacturonase produced by Rhizopus stolonifer. Biotechnol Lett 9: 219-224.
16. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31: 426-428.
17. Mishra C, S Keskar and M Rao. 1984. Production and properties of extracellular endoxylanase from Neurospora crassa. Appl Environ Microbiol 48: 224-228.
18. Nagel CW and MM Anderson. 1965. Action of a bacterial transeliminase on normal and unsaturated oligogalacturonic acids. Arch Biochem Biophys 112: 322-330.
19. O'Farrel PZ, HM Goodman and PH O'Farrel. 1977. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell 12: 1133-1142.
20. Pitt D. 1988. Pectin lyase from Phoma medicaginis var pinodella. In: Methods in Enzymology (Wood WA and ST Kelloggy, eds). Vol 161, pp 350-354, Academic Press, New York.
21. Polizeli MLTM, RCLR Pietro, JA Jorge and HF Terenzi. 1990. Effects of cell wall deficiency on the synthesis of polysaccharide-degrading exoenzymes. A study on mycelial and wall-less phenotypes of the fz. sg, os-I ('slime') triple mutant of Neurospora crassa. J Gen Microbiol 136: 1463-1468.
22. Polizeli MLTM, JA Jorge and HF Terenzi. 1991. Pectinase production by Neurospora crassa: purification and biochemical characterization of extracellular polygalacturonase activity. J Gen Microbiol 137: 1815-1823.
23. Rexová-Benková L and O Markovic. 1976. Pectic enzymes. Adv Carbohydr Chem Biochem 33: 323-385.
24. Rombouts FM and W Pilnik. 1980. Pectic enzymes. In: Economic Microbiology (Rose AH, ed). Vol 5, pp 227-282. Academic Press, New York.
25. Sigmund RD, MT McNally, DB Lee and SJ Free. 1985. Neurospora glucamylase and a mutant affected in its regulation. Biochem Genet 23: 89-103.
26. Swank RW and KD Munkres. 1971. Molecular weight analysis of oligopeptides by electrophoresis in polyacrylamide gel with sodium dodecyl sulphate. Anal Biochem 39: 462-477.
27. Tuttobello R and PJ Mill. 1961. The pectic enzymes of Aspergillus niger. I. The production of active mixtures of pectic enzymes. Biochem J 79: 51-57.
28. Vogel HJ. 1964. Distribution of lysine pathways among fungi: evolutionary implications. Am Nat 98: 435-446.