Separation and microgram-scale isolation of sea urchin egg granules by electrophoresis in polyvinylpyrrolidone solution, using horizontal gel electrophoresis apparatus with fluorescence detector

Electrophoresis

Volumn 19, Issue 7, 1998, Pages 1195-1204

  Chen, Nong ^a   Radko, Sergey P ^a   Chrambach, Andreas ^a,b  

^a EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Horizontal gel electrophoresis; Isolation; Polymer solution; Subcellular particle

Indexed keywords

CELL MEMBRANES; CYTOLOGY; FLUORESCENCE; GRANULATION; LIGHT SCATTERING; MAMMALS; SHELLFISH; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS;

FLUORESCENCE DETECTOR; GEL ELECTROPHORESIS; HORIZONTAL GEL ELECTROPHORESIS; ISOLATION; MEMBRANE VESICLES; POLYVINYLPYRROLIDONES; SEA URCHIN EGGS; SEA-URCHIN; SUB-CELLULAR; SUBCELLULAR PARTICLE;

ELECTROPHORESIS;

POVIDONE;

AUTOMATION; CELL GRANULE; CONFERENCE PAPER; FLUORESCENCE; GEL ELECTROPHORESIS; HOMOGENATE; NONHUMAN; SEA URCHIN;

EID: 0031807168     PISSN: 01730835     EISSN: None     Source Type: Journal    
DOI: 10.1002/elps.1150190721     Document Type: Conference Paper

References (36)

1. Vogel, S. S., Zimmerberg, J., Proc. Natl. Acad. Sci. USA 1992, 89, 4749-4753.
2. Zimmerberg, J., Vogel, S. S., Chernomordik, L. V., Annu. Rev. Biophys. Biomol. Struct. 1993, 22, 433-466.
3. Gombocz, E., Cortez, E., Appl. Theor. Electrophor. 1995, 4, 197-209.
4. Chang, H.-T., Chrambach, A., Appl. Theor. Electrophoresis 1995, 5, 73-77.
5. Chang, H.-T., Zakharov, S. F., Chrambach, A., Electrophoresis 1996, 17, 776-780.
6. Chen, N., Chrambach, A., Electrophoresis 1998, 19, in press.
7. Chen, N., Chrambach, A., Elecrrophoresis 1997, 18, 781-783.
8. Bauer, J., Weber, C., Electrophoresis 1996, 17, 526-528.
9. Heiger, D. N., Cohen, A. S., Karger, B. L., J. Chromatogr. 1990, 516, 33-48.
10. Preece, A. W., Brown, K. A., Adv. Electrophoresis 1989, 3, 351-404.
11. Gombocz, E., Cortez, E., Application Note 8, LabIntelligence, Belmont, CA.
12. Chen, N., Chrambach, A., Anal. Biochem. 1996, 242, 64-67.
13. Yarmola, E., Chen, N., Yi, D., Chrambach, A., Electrophoresis 1998, 19, 206-211.
14. Chestkov, V. V., Radko, S. R, Cho, M.-S., Chrambach, A., Vogel, S. S., J. Biol. Chem. 1998, 273, 2445-2451.
15. Radko, S. P., Garner, M. M., Caiafa, G., Chrambach, A., Anal. Biochem. 1994, 223, 82-87.
16. Radko, S. P., Chrambach, A., Appl. Theor. Electrophoresis 1995, 5, 79-88.
17. Radko, S. P., Chrambach, A., Electrophoresis 1996, 17, 1094-1102.
18. Radko, S. P., Sokoloff, A. V., Garner, M. M., Chrambach, A., Electrophoresis 1995, 16, 981-992.
19. Chang, H.-T., Chen, N., Sullivan, J. V., Chrambach, A., Anal. Biochem. 1997, 247, 111-114.
20. Tietz, D., Aldroubi, A., Pulyaeva, H., Guszczynski, T., Electrophoresis 1992, 13, 614-615.
21. Radko, S. P., Chrambach, A., Biopolymers 1997, 42, 183-189.
22. Radko, S. P., Chrambach, A., J. Phys. Chem. 1996, 100, 19461-19465.
23. Chrambach, A., The Practice of Quantitative Gel Electrophoresis, VCH, Weinheim 1985, pp. 1-265.
24. Tietz, D., Chrambach, A., Electrophoresis 1992, 13, 286-294.
25. Tietz, D., Chrambach, A., Anal. Biochem. 1987, 161, 395-411.
26. Rodbard, D., Chrambach, A., Weiss, G. H., in: Allen, R. C., Maurer, H. R. (Eds.), Electrophoresis and Isoelectric Focusing on Polyacrylamide Gel, Walter de Gruyter, Berlin 1974, pp. 28-62.
27. Laemmli, U. K., Nature 1970, 227, 680-685.
28. Cussler, E. L., Diffusion: Mass transfer in fluid systems, Cambridge University Press, Cambridge 1984.
29. Righetti, P. G., Caglio, S., Saracchi, M., Quaroni, S., Electrophoresis 1992, 13, 587-594.
30. Harrington, M. G., Lee, K. H., Bailey, J. E., Hood, L. E., Electrophoresis 1994, 15, 187-194.
31. Hannig, K., Wirth, H., Meyer, B.-H., Zeiller, K., Z. Physiol. Chem. 1975, 356, 1209-1223.
32. Righetti, P. G., Faupel, M., Wenisch, E., Adv. Electrophoresis 1992, 5, 159-200.
33. Weber, G., Boček, P., Electrophoresis 1996, 17, 1906-1910.
34. Just, W. W., Leon, V. J. O., Werner, G., Anal. Biochem. 1975, 67, 590-598.
35. Rodbard, D., in: Catsimpoolas, N. (Ed.), Methods of Protein Separation, Plenum Press, New York, NY 1976, Vol. 2, pp. 145-218.
36. Tietz, D., Chrambach, A., Electrophoresis 1993, 14, 185-190.