Overproduction and secretion of β-glucosidase in Bacillus subtilis

Journal of Microbiology and Biotechnology

Volumn 8, Issue 2, 1998, Pages 141-145

  Kim, Jeong Hyun ^a,c   Lee, Baek Rak ^b   Pack, Moo Young ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Inje University   (South Korea)

^c Samyang Corporation   (South Korea)

Author keywords

Glucosidase; Bacillus subtilis; Endoglucanase; Overproduction; Secretion

Indexed keywords

BETA GLUCOSIDASE;

ARTICLE; BACILLUS SUBTILIS; ENZYME RELEASE; ENZYME SYNTHESIS; GENE FUSION; GENE OVEREXPRESSION; GENE SEQUENCE; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION;

BACILLUS SUBTILIS; CELLULOMONAS FIMI;

EID: 0031805068     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (14)

1. Bradford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
2. Choi, Y. J., C. J. Kim, S. Y. Park, Y. T. Ko, H. K. Jeong, and G. E. Ji. 1996. Growth and β-glucosidase activity of Bifidobacterium. J. Microbiol. Biotechnol. 6: 255-259.
3. Chun, S. B., D. H. Kim, K. H. Kim, and K. C. Chung. 1991. Purification and characterization of β-glucosidase from Penicillium verruculosum. J. Microbiol. Biotechnol. 1: 188-196.
4. Kawamura, F. and R. H. Doi. 1984. Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral protease. J. Bacteriol. 160: 442-444.
5. Kim, H. K. 1988. Cloning and expression of Cellulomonas fimi β-glucosidase genes in Escherichia coli. Ph. D. Thesis, KAIST, Seoul.
6. Kim, J. H. and M. Y. Pack. 1993. Overproduction of extracellular endoglucanase by genetically engineered Bacillus subtilis. Biotechnol. Lett. 15: 133-138.
7. Lee, B. R., J. H. Kim and M. Y. Pack. 1994. The influence of mRNA secondary structure on expression of endoglucanase in Bacillus subtilis. Biotechnol. Lett. 16: 1001-1006.
8. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
9. Miller, G. L. 1959. Measurements of reducing sugars by DNS reagent method. Anal. Chem. 31: 426-428.
10. Reese, E. T. and M. Mandels. 1984. Rolling with the times: Production and applications of Trichoderma reesei cellulase. pp. 1-20. In Tsao G (ed.) Ann. Rep. Ferm. Proc. vol. 7. Academic Press, New York.
11. Sheldon, J. B., D. G. Cooper, and O. M. Fuller. 1987. Effect of media composition and growth conditions on production of cellulase and β-glucosidase by a mixed fungal fermentation. Enzyme Microb. Technol. 9: 47-52.
12. Shewale, J. G. 1982. β-Glucosidase: Its role in cellulase synthesis and hydrolysis of cellulose. Int. J. Biochem. 14: 435-443.
13. Spizizen, J. 1958. Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc. Natl. Acad. Sci. USA 44: 1072-1078.
14. Tangnu, S. K., H. W. Blanch, and C. R. Wilke. 1981. Enhanced production of cellulase, hemicellulase, and β-glucosidase by Trichoderma reesei. Biotechnol. Bioeng. 23: 1837-1849.