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Volumn 5, Issue 5, 1998, Pages 684-691

Generation of a conditionally neo'-containing retroviral producer cell line: Effects of neo' on retroviral titer and transgene expression

Author keywords

Cre locP; GFP; Neo'; Singel gene retroviral vectors

Indexed keywords

GREEN FLUORESCENT PROTEIN; NEOMYCIN;

EID: 0031803564     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300654     Document Type: Article
Times cited : (14)

References (53)
  • 1
    • 0029818322 scopus 로고    scopus 로고
    • The TMC Worldwide Gene Therapy Enrollment Report (June 1996)
    • Marcel T, Grausz JD. The TMC Worldwide Gene Therapy Enrollment Report (June 1996). Hum Gene Ther 1996; 7: 2025-2046.
    • (1996) Hum Gene Ther , vol.7 , pp. 2025-2046
    • Marcel, T.1    Grausz, J.D.2
  • 2
    • 0027595949 scopus 로고
    • The basic science of gene therapy
    • Mulligan RC. The basic science of gene therapy. Science 1993; 260: 926-932.
    • (1993) Science , vol.260 , pp. 926-932
    • Mulligan, R.C.1
  • 3
    • 0029164582 scopus 로고
    • Effects of retroviral vector design on expression of human adenosine deaminase in murine bone marrow transplant recipients engrafted with genetically modified cells
    • Riviere I, Brose K, Mulligan RC. Effects of retroviral vector design on expression of human adenosine deaminase in murine bone marrow transplant recipients engrafted with genetically modified cells. Proc Natl Acad Sci USA 1995; 92: 6733-6737.
    • (1995) Proc Natl Acad Sci USA , vol.92 , pp. 6733-6737
    • Riviere, I.1    Brose, K.2    Mulligan, R.C.3
  • 4
    • 0024372137 scopus 로고
    • Factors affecting long-term stability of Moloney murine leukemia virus-based vectors
    • Xu L, Yee JK, Wolff JA, Friedmann T. Factors affecting long-term stability of Moloney murine leukemia virus-based vectors. Virology 1989; 171: 331-341.
    • (1989) Virology , vol.171 , pp. 331-341
    • Xu, L.1    Yee, J.K.2    Wolff, J.A.3    Friedmann, T.4
  • 5
    • 0021726790 scopus 로고
    • Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism
    • Emerman M, Temin HM. Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism. Cell 1984; 39: 449-467.
    • (1984) Cell , vol.39 , pp. 449-467
    • Emerman, M.1    Temin, H.M.2
  • 6
    • 0025766798 scopus 로고
    • Promoter interactions in retrovirus vectors introduced into fibroblasts and embryonic stem cells
    • Soriano P, Friedrich G, Lawinger P. Promoter interactions in retrovirus vectors introduced into fibroblasts and embryonic stem cells. J Virol 1991; 65: 2314-2319.
    • (1991) J Virol , vol.65 , pp. 2314-2319
    • Soriano, P.1    Friedrich, G.2    Lawinger, P.3
  • 7
    • 0025824001 scopus 로고
    • Retroviral gene transfer of human adenosine deaminase in murine hematopoietic cells: Effect of selectable marker sequences on long-term expression
    • Apperley JF, Luskey BD, Williams DA. Retroviral gene transfer of human adenosine deaminase in murine hematopoietic cells: effect of selectable marker sequences on long-term expression. Blood 1991; 78: 310-317.
    • (1991) Blood , vol.78 , pp. 310-317
    • Apperley, J.F.1    Luskey, B.D.2    Williams, D.A.3
  • 8
    • 0028145310 scopus 로고
    • Retroviral vector design for long-term expression in murine hematopoietic cells in vivo
    • Correll PH, Colilla S, Karlsson S. Retroviral vector design for long-term expression in murine hematopoietic cells in vivo. Blood 1994; 84: 1812-1822.
    • (1994) Blood , vol.84 , pp. 1812-1822
    • Correll, P.H.1    Colilla, S.2    Karlsson, S.3
  • 9
    • 0029794429 scopus 로고    scopus 로고
    • Analysis of the relative level of gene expression from different retroviral vectors used for gene therapy
    • Byun J et al. Analysis of the relative level of gene expression from different retroviral vectors used for gene therapy. Gene Therapy 1996; 3: 780-788.
    • (1996) Gene Therapy , vol.3 , pp. 780-788
    • Byun, J.1
  • 10
    • 0029965285 scopus 로고    scopus 로고
    • Increased levels of spliced RNA account for augmented expression from the MFG retroviral vector in hematopoietic cells
    • Krall W et al. Increased levels of spliced RNA account for augmented expression from the MFG retroviral vector in hematopoietic cells. Gene Therapy 1996; 3: 37-48.
    • (1996) Gene Therapy , vol.3 , pp. 37-48
    • Krall, W.1
  • 11
    • 0019989719 scopus 로고
    • Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter
    • Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet 1982; 1: 327-341.
    • (1982) J Mol Appl Genet , vol.1 , pp. 327-341
    • Southern, P.J.1    Berg, P.2
  • 12
    • 0025908566 scopus 로고
    • The prokaryotic neomycin-resistance-encoding gene acts as a transcriptional silencer in eukaryotic cells
    • Artelt P et al. The prokaryotic neomycin-resistance-encoding gene acts as a transcriptional silencer in eukaryotic cells. Gene 1991; 99: 249-254.
    • (1991) Gene , vol.99 , pp. 249-254
    • Artelt, P.1
  • 13
    • 0023832621 scopus 로고
    • The expression of neomycin phosphotransferase in human promyelocytic leukemia cells (HL60) delays their differentiation
    • von Melchner H, Housman DE. The expression of neomycin phosphotransferase in human promyelocytic leukemia cells (HL60) delays their differentiation. Oncogene 1988; 2: 137-140.
    • (1988) Oncogene , vol.2 , pp. 137-140
    • Von Melchner, H.1    Housman, D.E.2
  • 14
    • 0028129054 scopus 로고
    • Expression of the neomycin-resistance (neo) gene induces alterations in gene expression and metabolism
    • Valera A, Perales JC Hatzoglou M, Bosch F. Expression of the neomycin-resistance (neo) gene induces alterations in gene expression and metabolism. Hum Gene Ther 1994; 5: 449-456.
    • (1994) Hum Gene Ther , vol.5 , pp. 449-456
    • Valera, A.1    Perales, J.C.2    Hatzoglou, M.3    Bosch, F.4
  • 15
    • 13344261952 scopus 로고    scopus 로고
    • T cell-mediated rejection of gene-modified HIV-specific cytotoxic T lymphocytes in HIV-infected patients
    • Riddell S et al. T cell-mediated rejection of gene-modified HIV-specific cytotoxic T lymphocytes in HIV-infected patients. Nature Med 1996; 2: 216-223.
    • (1996) Nature Med , vol.2 , pp. 216-223
    • Riddell, S.1
  • 16
    • 0030843493 scopus 로고    scopus 로고
    • HSVtk gene transfer into donor lymphocytes for control of allogeneic graft-versus-leukemia
    • Bonini C et al. HSVtk gene transfer into donor lymphocytes for control of allogeneic graft-versus-leukemia. Science 1997; 276: 1719-1724.
    • (1997) Science , vol.276 , pp. 1719-1724
    • Bonini, C.1
  • 17
    • 0019888362 scopus 로고
    • Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites
    • Sternberg N, Hamilton D. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. J Mol Biol 1981; 150: 467-486.
    • (1981) J Mol Biol , vol.150 , pp. 467-486
    • Sternberg, N.1    Hamilton, D.2
  • 18
    • 0019888368 scopus 로고
    • Bacteriophage P1 site-specific recombination. II. Recombination between loxP and the bacterial chromosome
    • Sternberg N, Hamilton D, Hoess R. Bacteriophage P1 site-specific recombination. II. Recombination between loxP and the bacterial chromosome. J Mol Biol 1981; 150: 487-507.
    • (1981) J Mol Biol , vol.150 , pp. 487-507
    • Sternberg, N.1    Hamilton, D.2    Hoess, R.3
  • 19
    • 0021764162 scopus 로고
    • Site-specific recombination by the bacteriophage P1 lox-Cre system. Cre-mediated synapsis of two lox sites
    • Hamilton DL, Abremski K. Site-specific recombination by the bacteriophage P1 lox-Cre system. Cre-mediated synapsis of two lox sites. J Mol Biol 1984; 178: 481-486.
    • (1984) J Mol Biol , vol.178 , pp. 481-486
    • Hamilton, D.L.1    Abremski, K.2
  • 20
    • 0028358919 scopus 로고
    • Recycling selectable markers in yeast
    • Sauer B. Recycling selectable markers in yeast. Biotechniques 1994; 16: 1086-1088.
    • (1994) Biotechniques , vol.16 , pp. 1086-1088
    • Sauer, B.1
  • 21
    • 0030881446 scopus 로고    scopus 로고
    • A simple and reliable method for screening retroviral producer clones without selectable markers
    • Onodera M et al. A simple and reliable method for screening retroviral producer clones without selectable markers. Hum Gene Ther 1997; 8: 1189-1194.
    • (1997) Hum Gene Ther , vol.8 , pp. 1189-1194
    • Onodera, M.1
  • 22
    • 0030879640 scopus 로고    scopus 로고
    • Green fluorescent protein retroviral vectors: Low titer and high recombination frequency suggest a selective disadvantage
    • Hanazono Y, Yu JM, Dunbar CE, Emmons RVB. Green fluorescent protein retroviral vectors: low titer and high recombination frequency suggest a selective disadvantage. Hum Gene Ther 1997; 8: 1313-1319.
    • (1997) Hum Gene Ther , vol.8 , pp. 1313-1319
    • Hanazono, Y.1    Yu, J.M.2    Dunbar, C.E.3    Emmons, R.V.B.4
  • 23
    • 0024509412 scopus 로고
    • Cre-stimulated recombination at loxP-containing DNA sequences placed into the mammalian genome
    • Sauer B, Henderson N. Cre-stimulated recombination at loxP-containing DNA sequences placed into the mammalian genome. Nucleic Acids Res 1989; 17: 147-161.
    • (1989) Nucleic Acids Res , vol.17 , pp. 147-161
    • Sauer, B.1    Henderson, N.2
  • 24
    • 0025360095 scopus 로고
    • Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase
    • Sauer B, Henderson N. Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biol 1990; 2: 441-449.
    • (1990) New Biol , vol.2 , pp. 441-449
    • Sauer, B.1    Henderson, N.2
  • 25
    • 0023511728 scopus 로고
    • Site-specific insertion of DNA into a pseudorabies virus vector
    • Sauer B, Whealy M, Robbins A, Enquist L. Site-specific insertion of DNA into a pseudorabies virus vector. Proc Natl Acad Sci USA 1987; 84: 9108-9112.
    • (1987) Proc Natl Acad Sci USA , vol.84 , pp. 9108-9112
    • Sauer, B.1    Whealy, M.2    Robbins, A.3    Enquist, L.4
  • 26
    • 0030025850 scopus 로고    scopus 로고
    • Transfer of single gene-containing long terminal repeats into the genome of mammalian cells by a retroviral vector carrying the cre gene and the loxP site
    • Choulika A, Guyot V, Nicolas JF. Transfer of single gene-containing long terminal repeats into the genome of mammalian cells by a retroviral vector carrying the cre gene and the loxP site. J Virol 1996; 70: 1792-1798.
    • (1996) J Virol , vol.70 , pp. 1792-1798
    • Choulika, A.1    Guyot, V.2    Nicolas, J.F.3
  • 27
    • 0030011519 scopus 로고    scopus 로고
    • Self-deleting retrovirus vectors for gene therapy
    • Russ AP, Friedel C, Grez M, von Melchner H. Self-deleting retrovirus vectors for gene therapy. J Virol 1996; 70: 4927-4932.
    • (1996) J Virol , vol.70 , pp. 4927-4932
    • Russ, A.P.1    Friedel, C.2    Grez, M.3    Von Melchner, H.4
  • 28
    • 0028856772 scopus 로고
    • Excision of specific DNA sequences from integrated retroviral vectors via site-specific recombination
    • Bergemann J et al. Excision of specific DNA sequences from integrated retroviral vectors via site-specific recombination. Nucleic Acids Res 1995; 23: 4451-4456.
    • (1995) Nucleic Acids Res , vol.23 , pp. 4451-4456
    • Bergemann, J.1
  • 29
    • 0030800730 scopus 로고    scopus 로고
    • Cre/loxP-mediated excision of a neomycin resistance expression unit from an integrated retroviral vector increases long terminal repeat-driven transcription in human hematopoietic cells
    • Fernex C, Dubreuil P, Mannoni P, Bagnis C. Cre/loxP-mediated excision of a neomycin resistance expression unit from an integrated retroviral vector increases long terminal repeat-driven transcription in human hematopoietic cells. J Virol 1997; 71: 7533-7540.
    • (1997) J Virol , vol.71 , pp. 7533-7540
    • Fernex, C.1    Dubreuil, P.2    Mannoni, P.3    Bagnis, C.4
  • 30
    • 0029810898 scopus 로고    scopus 로고
    • Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination
    • Westerman KA, Leboulch P. Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc Natl Acad Sci USA 1996; 93: 8971-9876.
    • (1996) Proc Natl Acad Sci USA , vol.93 , pp. 8971-9876
    • Westerman, K.A.1    Leboulch, P.2
  • 31
    • 23444431611 scopus 로고
    • Green fluorescent protein as a marker for gene expression
    • Chalfie M et al. Green fluorescent protein as a marker for gene expression. Science 1994; 263: 802-805.
    • (1994) Science , vol.263 , pp. 802-805
    • Chalfie, M.1
  • 32
    • 0030595059 scopus 로고    scopus 로고
    • Quantitative analysis of transient gene expression in mammalian cells using the green fluorescent protein
    • Subramanian S, Srienc F. Quantitative analysis of transient gene expression in mammalian cells using the green fluorescent protein. J Biotechnol 1996; 49: 137-151.
    • (1996) J Biotechnol , vol.49 , pp. 137-151
    • Subramanian, S.1    Srienc, F.2
  • 33
    • 0021281130 scopus 로고
    • Expression of genes introduced into cells by retroviral infection is more efficient than that of genes introduced into cells by DNA transfection
    • Hwang LH, Gilboa E. Expression of genes introduced into cells by retroviral infection is more efficient than that of genes introduced into cells by DNA transfection. J Virol 1984; 50: 417-424.
    • (1984) J Virol , vol.50 , pp. 417-424
    • Hwang, L.H.1    Gilboa, E.2
  • 34
    • 0027613280 scopus 로고
    • High frequency expression of integrated proviruses derived from enhancer trap retroviruses
    • Sablitzky F, Jonsson JI, Cohen BL, Phillips RA. High frequency expression of integrated proviruses derived from enhancer trap retroviruses. Cell Growth Diff 1993; 4: 451-459.
    • (1993) Cell Growth Diff , vol.4 , pp. 451-459
    • Sablitzky, F.1    Jonsson, J.I.2    Cohen, B.L.3    Phillips, R.A.4
  • 35
    • 0027385517 scopus 로고
    • A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses
    • Duch M et al. A correlation between dexamethasone inducibility and basal expression levels of retroviral vector proviruses. Nucleic Acids Res 1993; 21: 4777-4782.
    • (1993) Nucleic Acids Res , vol.21 , pp. 4777-4782
    • Duch, M.1
  • 36
    • 0027538181 scopus 로고
    • Vaccination with irradiated tumor cells engineered to secrete murine granulocyte-macrophage colony-stimulating factor stimulates potent, specific, and long-lasting anti-tumor immunity
    • Dranoff G et al. Vaccination with irradiated tumor cells engineered to secrete murine granulocyte-macrophage colony-stimulating factor stimulates potent, specific, and long-lasting anti-tumor immunity. Proc Natl Acad Sci USA 1993; 90: 3539-3543.
    • (1993) Proc Natl Acad Sci USA , vol.90 , pp. 3539-3543
    • Dranoff, G.1
  • 37
    • 0023904379 scopus 로고
    • Comparison of expression in hemopoietic cells by retroviral vectors carrying two genes
    • Bowtell DD, Cory S, Johnson GR, Gonda TJ. Comparison of expression in hemopoietic cells by retroviral vectors carrying two genes. J Virol 1988; 62: 2464-2473.
    • (1988) J Virol , vol.62 , pp. 2464-2473
    • Bowtell, D.D.1    Cory, S.2    Johnson, G.R.3    Gonda, T.J.4
  • 38
    • 0028813149 scopus 로고
    • Interleukin-2-transduced lymphocytes grow in an autocrine fashion and remain responsive to antigen
    • Treisman J et al. Interleukin-2-transduced lymphocytes grow in an autocrine fashion and remain responsive to antigen. Blood 1995; 85: 139-145.
    • (1995) Blood , vol.85 , pp. 139-145
    • Treisman, J.1
  • 39
    • 0026588638 scopus 로고
    • Retroviral vectors containing putative internal ribosome entry sites: Development of a polycistronic gene transfer system and applications to human gene therapy
    • Morgan RA et al. Retroviral vectors containing putative internal ribosome entry sites: development of a polycistronic gene transfer system and applications to human gene therapy. Nucleic Acids Res 1992; 20: 1293-1299.
    • (1992) Nucleic Acids Res , vol.20 , pp. 1293-1299
    • Morgan, R.A.1
  • 40
    • 0029043548 scopus 로고
    • Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: A molecular switch for control of gene expression
    • Anton M, Graham FL. Site-specific recombination mediated by an adenovirus vector expressing the Cre recombinase protein: a molecular switch for control of gene expression. J Virol 1995; 69: 4600-4606.
    • (1995) J Virol , vol.69 , pp. 4600-4606
    • Anton, M.1    Graham, F.L.2
  • 41
    • 0028559768 scopus 로고
    • An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3
    • Bett AJ, Haddara W, Prevec L, Graham FL. An efficient and flexible system for construction of adenovirus vectors with insertions or deletions in early regions 1 and 3. Proc Natl Acad Sci USA 1994; 91: 8802-8806.
    • (1994) Proc Natl Acad Sci USA , vol.91 , pp. 8802-8806
    • Bett, A.J.1    Haddara, W.2    Prevec, L.3    Graham, F.L.4
  • 42
    • 0029315806 scopus 로고
    • Methods for construction of adenovirus vectors
    • Graham FL, Prevec L. Methods for construction of adenovirus vectors. Mol Biotechnol 1995; 3: 207-220.
    • (1995) Mol Biotechnol , vol.3 , pp. 207-220
    • Graham, F.L.1    Prevec, L.2
  • 43
    • 0024460181 scopus 로고
    • Improved retroviral vectors for gene transfer and expression
    • Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. Biotechniques 1989; 7: 980-982.
    • (1989) Biotechniques , vol.7 , pp. 980-982
    • Miller, A.D.1    Rosman, G.J.2
  • 44
    • 0024239024 scopus 로고
    • Construction and use of a safe and efficient amphotropic packaging cell line
    • Markowitz D, Goff S, Bank A. Construction and use of a safe and efficient amphotropic packaging cell line. Virology 1988; 167: 400-406.
    • (1988) Virology , vol.167 , pp. 400-406
    • Markowitz, D.1    Goff, S.2    Bank, A.3
  • 45
    • 0022767368 scopus 로고
    • Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production
    • Miller AD, Buttimore C. Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol Cell Biol 1986; 6: 2895-2902.
    • (1986) Mol Cell Biol , vol.6 , pp. 2895-2902
    • Miller, A.D.1    Buttimore, C.2
  • 46
    • 0017710978 scopus 로고
    • Characteristics of a human cell line transformed by DNA from human adenovirus type 5
    • Graham FL, Smiley J, Russell WC, Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol 1977; 36: 59-74.
    • (1977) J Gen Virol , vol.36 , pp. 59-74
    • Graham, F.L.1    Smiley, J.2    Russell, W.C.3    Nairn, R.4
  • 47
    • 0014604721 scopus 로고
    • Murine sarcoma and leukemia viruses: Assay using clonal lines of contact-inhibited mouse cells
    • Jainchill JL, Aaronson SA, Todaro GJ. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J Virol 1969; 4: 549-553.
    • (1969) J Virol , vol.4 , pp. 549-553
    • Jainchill, J.L.1    Aaronson, S.A.2    Todaro, G.J.3
  • 48
    • 0018800121 scopus 로고
    • Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure
    • Kafatos FC, Jones CW, Efstratiadis A. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. Nucleic Acids Res 1979; 7: 1541-1552.
    • (1979) Nucleic Acids Res , vol.7 , pp. 1541-1552
    • Kafatos, F.C.1    Jones, C.W.2    Efstratiadis, A.3
  • 49
    • 0024284028 scopus 로고
    • A simple salting out procedure for extracting DNA from human nucleated cells
    • Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988; 16: 1215.
    • (1988) Nucleic Acids Res , vol.16 , pp. 1215
    • Miller, S.A.1    Dykes, D.D.2    Polesky, H.F.3
  • 50
    • 0018598319 scopus 로고
    • Gel electrophoresis of restriction fragments
    • Southern E. Gel electrophoresis of restriction fragments. Methods Enzymol 1979; 68: 152-176.
    • (1979) Methods Enzymol , vol.68 , pp. 152-176
    • Southern, E.1
  • 51
    • 0020793569 scopus 로고
    • A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
    • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 1983; 132: 6-13.
    • (1983) Anal Biochem , vol.132 , pp. 6-13
    • Feinberg, A.P.1    Vogelstein, B.2
  • 53
    • 0030817018 scopus 로고    scopus 로고
    • Development of high-titer retroviral producer cell lines by using Cre-mediated recombination
    • Vanin EF et al. Development of high-titer retroviral producer cell lines by using Cre-mediated recombination. J Virol 1997; 71: 7820-7826.
    • (1997) J Virol , vol.71 , pp. 7820-7826
    • Vanin, E.F.1


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