Mounting technique allows observation of immuno-labeled cells on plastic coverslips

BioTechniques

Volumn 24, Issue 6, 1998, Pages 910-914

  O'Donohue, Marie Françoise ^a,b   Cheutin, T ^a   Klein, C ^a   Kaplan, H ^a   Ploton, D ^a  

^a INSERM   (France)

^b HÔPITAL MAISON BLANCHE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

KI 67 ANTIGEN; PLASTIC;

ANTIGEN DETECTION; DNA DETERMINATION; IMMUNOFLUORESCENCE TEST; SHORT SURVEY;

EID: 0031802027     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98246bm01     Document Type: Short Survey

References (8)

1. Brugal, G. 1995. Quantitative microscopy and tumor cell proliferation. Bull. Cancer 511S- 517S.
2. Doyle, A., J.B. Griffiths and D.G. Newell. 1993. Cell and Tissue Culture: Laboratory Procedures. John Wiley & Sons International, Chichester, UK.
3. Gerdes, J., U. Schwab, H. Lemke and H. Stein. 1983. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. Int. J. Cancer 37:13-20.
4. Gilbert, N., L. Lucas, C. Klein, M. Ménager, N. Bonnet and D. Ploton. 1995. Three-dimensional co-location of RNA polymerase I and DNA during interphase and mitosis by confocal microscopy. J. Cell Sci. 108: 115- 125.
5. Héliot, L., H. Kaplan, L. Lucas, C. Klein, A. Beorchia, M. Doco-Fenzy, M. Ménager, M. Thiry, M.F. O'Donohue and D. Ploton. 1997. Electron-tomography of metaphase nucleolar organizer regions (NORs): evidence for a twisted-loop organisation, Mol. Biol. Cell 8:2199-2216.
6. Mazerolles, C., P. Rishmann, D. Chopin, Z. Popov, B. Maluraud, J. Selves, I. Neulat- Duga, J. Bellot and G. Delsol. 1994. Usefulness of Mib1 monoclonal antibody in assessing the proliferative index in human bladder carcinoma: comparison with Ki-67 antibody. Histopathology 25:563-568.
7. .Verheijen, R., H.J.H. Kuijpers, R.O. Schlingemann, A.L.M. Boehmer, R. van Driel, G.J. Brakennoff and F.C.S. Ramaekers. 1989. Ki-67 detects a nuclear matrix-associated proliferation-related antigen. I. Intracellular localization during interphase. J. Cell Sci. 92:123-130.
8. Verheijen, R., H.J.H. Kuijpers, R. van Driel, J.L.M. Beck, J.H. van Dierendonck, G.J. Brakenhoff and F.C.S. Ramaekers. 1989. Ki-67 detects a nuclear matrix-associated proliferation-related antigen. II. Localization in mitotic cells and association with chromosomes. J. Cell Sci. 92:531-540.