X-gal staining of Drosophila embryos compatible with anti-body staining or in situ hybridization

BioTechniques

Volumn 24, Issue 6, 1998, Pages 918-922

  Su, Ming Tsan ^a   Golden, Krista ^a   Bodmer, Rolf ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL TISSUE; CONTROLLED STUDY; DROSOPHILA; EMBRYO; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; NONHUMAN; SHORT SURVEY; STAINING;

ANIMALIA; HEXAPODA;

EID: 0031801384     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98246bm03     Document Type: Short Survey

References (9)

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3. Bier, E., H. Vaessin, S. Shepherd, K. Lee, K. McCall, S. Barbel, L. Ackerman, R, Carretto et al. 1989. Search for pattern and mutation in the Drosophila genome with a P-lacZ vector. Genes Dev. 3:1273-1287.
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5. Hiromi, Y., A. Kuroiwa and W.J. Gehring. 1985. Control elements of the Drosophila segmentation gene fushi-tarazu. Cell 43:603-613.
6. Kobayashi, S. and M. Okada. 1993. A double staining technique using 5-bromo, 4- chloro-3-indolyl-β-D-galactopyranoside (X- gal) and immunoperoxidase in whole Drosophila embryos. Biotech. Histochem. 68:237-239.
7. O'Kane, C. and W.J. Gehring. 1987. Detection in situ of genomic regulatory elements in Drosophila. Proc. Natl. Acad. Sci. USA 84:9123-9127.
8. Singh, A., M. Kango and P. Sinha. 1995. An improved method for chemical devitellinization of X-gal stained Drosophila embryos. Indian J. Exp. Biol. 33:150-152.
9. Tautz, D. and D. Pfeifle. 1989, A nonradioactive in situ hybridization method for the localization of specific RNAs in Drosophila reveals a translational control of the segmentation gene hunchback. Chromosoma 98:81-85.