Improvement of HLA class I and class II PCR-SSP typing by using timed-release activity of DNA polymerase

Tissue Antigens

Volumn 51, Issue 6, 1998, Pages 645-648

  Chen, D F ^a,b   Seibert, I ^a   Chen, H Y ^a   Herbst Kiene, I ^a   Pastucha, L T ^a   Stangel, W ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

HLA typing; Hot start PCR; PCR SSP; Timed release activity of DNA polymerase

Indexed keywords

DNA POLYMERASE; HLA ANTIGEN CLASS 1; HLA ANTIGEN CLASS 2; HLA B ANTIGEN;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE AMPLIFICATION; HLA TYPING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

EID: 0031801080     PISSN: 00012815     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1399-0039.1998.tb03007.x     Document Type: Article

References (4)

1. Olerup O, Zetterquist H. HLA-DR typing by PCR amplification with sequence-specific primers (PCR-SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipients matching in cadaveric transplantation. Tissue Antigens 1992: 39: 225-35.
2. Chou Q, Russell M, Birch DE, Raymond J, Bloch W. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res 1993: 20: 1717-23.
3. Chen D-F, Pastucha LT, Chen H-Y, Kadar JG, Stangel W. Simultaneous genotyping of human platelet antigens by hot start sequence-specific polymerase chain reaction with DNA polymerase AmpliTaq Gold. Vox Sang 1997: 72: 192-6.
4. Kellogg DE, Rybalkin I, Chen S et al. TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. Biotechniques 1994: 16: 1134-7.