Analytical and clinical performance of a homogeneous enzymatic LDL- cholesterol assay compared with the ultracentrifugation-dextran sulfate- Mg2+ method

Clinical Chemistry

Volumn 44, Issue 6, 1998, Pages 1242-1250

  Rifai, Nader ^a,b   Iannotti, Elizabeth ^a   Deangelis, Kristen ^a   Law, Terence ^a  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HIGH DENSITY LIPOPROTEIN CHOLESTEROL; LOW DENSITY LIPOPROTEIN CHOLESTEROL; TRIACYLGLYCEROL;

ARTICLE; CENTRIFUGAL ANALYZER; CHOLESTEROL BLOOD LEVEL; DIAGNOSTIC ACCURACY; ENZYME IMMUNOASSAY; HUMAN; HYPERCHOLESTEROLEMIA; LIPID ANALYSIS; MAJOR CLINICAL STUDY; ULTRACENTRIFUGATION;

EID: 0031800741     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/44.6.1242     Document Type: Article

References (19)

1. The Expert Panel. Summary of the second report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel II). JAMA 1993;269:3015-23.
2. Friedewald WT, Levy RT, Fredrickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma without use of the ultracentrifuge. Clin Chem 1972;18:449-502.
3. Rifai N, Warnick GR, McNamara JR, Belcher JD, Grinstead GF, Frantz ID Jr. Measurement of low density lipoprotein cholesterol in serum: a status report. Clin Chem 1992;38:150-60.
4. Bachorik PS. Measurement of low density lipoprotein cholesterol. In: Rifai N, Warnick GR, Dominiczak MH, eds. Handbook of lipoprotein testing. Washington, DC: AACC Press, 1997:145-60.
5. Bachorik PS, Ross JW, for the National Cholesterol Education Program Working Group on Lipoprotein Measurement. Guidelines on the measurement of low density lipoprotein cholesterol: executive summary. Clin Chem 195;44:1414-20.
6. Harris N, Neufeld EJ, Newberger JW, Ticho B, Baker A, Ginsburg GS, et al. Analytical performance and clinical utility of a direct LDL-cholesterol assay in a hyperlipidemic pediatric population. Clin Chem 1996;42:1182-8.
7. Yu HH, Ginsburg GS, Harris N, Rifai N. Evaluation and clinical application of a direct low density lipoprotein cholesterol assay in normolipidemic and hyperlipidemic adults. Am J Cardiol 1997;80: 1295-9.
8. Sheikh M, Miller NE. Evaluation of commercial reagent for precipitating human serum low density lipoprotein. Clin Chim Acta 1985;152:213-7.
9. Demacker PN, Hijmans AG, Brenninkmeijer BJ, Jansen AP, van t'Laar A. Five methods for determining low density lipoprotein cholesterol compared. Clin Chem 1984;30:1797-800.
10. Assmann G, Jabs HU, Nolte W, Schriewer H. Precipitation of LDL with sulphopolyanions: a comparison of two methods for LDL cholesterol determination. J Clin Chem Clin Biochem 1984;22: 781-5.
11. Mulder K, van Leeuwen C, Schouten JA, van Geut CM, Lahey J, van der Hoort HA. An evaluation of three commercial methods for the determination of LDL cholesterol. Clin Chim Acta 1984;143:29-35.
12. Cole TG, Ferguson CA, Gibson DW. High-throughput beta-quantification methods. Atherosclerosis 1994;108(Suppl):S191.
13. 2+ precipitation procedure for quantitation of high density lipoprotein cholesterol. In: Cooper GR, ed. Selected methods of clinical chemistry. Vol. 10. Washington, DC: American Association for Clinical Chemistry, 1983:91-9.
14. Washko PW, Welch RW, Dhariwal KR, Wang Y, Levine M. Ascorbic acid and dehydroascorbic acid analyses in biological samples. Anal Biochem 1992;204:1-14.
15. Carey RN, Garber CC. Evaluation of methods. In: Kaplan LA, Pecse AJ, eds. Clinical chemistry: theory, analysis, and correlation, 3rd ed. St. Louis: CV Mosby Co., 1996:402-23.
16. Westgard JO, Carey RN, Wold S. Criteria for judging precision and accuracy in method development and evaluation. Clin Chem 1974;20:825-33.
17. Cloey T, Bachorik PS, Becker D, Finney C, Lawry D, Sigmund W. Reevaluation of serum-plasma differences in total cholesterol concentration. JAMA 1990;263:2788-9.
18. Naito HK. Problems associated with lipid and lipoprotein analyses. In: Widhalm K, Naito HK, eds. Detection and treatment of lipid and lipoprotein disorders of childhood. New York: Alan R. Liss, 1985:19-60.
19. Cohn JS, McNamara JR, Schaefer EJ. Lipoprotein cholesterol concentrations in the plasma of human subjects as measured in the fed and fasted states. Clin Chem 1988;34:2456-9.