PCR-directed DNA sequencing of 'nondeletion' HPRT- mutants induced by bleomycin in CHO K1-BH4 cells

Environmental and Molecular Mutagenesis

Volumn 32, Issue 3, 1998, Pages 244-250

  An, Jie ^a,b   Trieff, Norman M ^a   Hsie, Abraham W ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

^b ELI LILLY AND COMPANY   (United States)

Author keywords

Bleomycin; CHO cells; DNA sequencing; HPRT mutants; K1 BH4 cells; Reactive oxygen species

Indexed keywords

BLEOMYCIN; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; REACTIVE OXYGEN METABOLITE; SUPEROXIDE DISMUTASE; TEMPOL; TRIENTINE;

ARTICLE; CHO CELL; DNA SEQUENCE; DNA STRAND BREAKAGE; MUTATION; SEQUENCE ANALYSIS;

ANIMALS; ANTIOXIDANTS; BASE SEQUENCE; BLEOMYCIN; CHO CELLS; CRICETINAE; CYCLIC N-OXIDES; DNA MUTATIONAL ANALYSIS; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; MUTAGENS; REACTIVE OXYGEN SPECIES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPIN LABELS; TRIETHYLENETETRAMINE;

CRICETINAE; CRICETULUS GRISEUS; RODENTIA;

EID: 0031796958     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2280(1998)32:3<244::AID-EM7>3.0.CO;2-U     Document Type: Article

References (24)

1. An J, Hsie AW (1992): Effects of an inhibitor and a mimic of Superoxide dismutase on bleomycin mutagenesis in Chinese hamster ovary cells. Mutat Res 270:167-175.
2. An J, Hsie AW (1993): Polymerase chain reaction-based deletion screening of bleomycin induced 6-thioguanine resistant mutants in Chinese hamster ovary cells - Effects of an inhibitor and a mimic of Superoxide dismutase. Mutat Res 289:215-222.
3. An J, Hsie AW (1994): Polymerase chain reaction-directed DNA sequencing of bleomycin-induced "nondeletion"-type, 6-thioguanine-resistant mutants in Chinese hamster ovary cell derivative AS52: Effects of an inhibitor and a mimic of Superoxide dismutase. Environ Mol Mutagen 23:101-109.
4. Bennett RAO, Swerdlow PS, Povirk LF (1993): Spontaneous cleavage of bleomycin-induced abasic sites in chromatin and their mutagenicity in mammalian shuttle vectors. Biochemistry 32:3188-3195.
5. Breen AP, Murphy JA (1995): Reactions of oxyl radicals with DNA. Free Rad Biol Med 18:1033-1077.
6. Burger RM, Peisach J, Horwitz SB (1981): Activated bleomycin. J Biol Chem 256:11636-11644.
7. Giloni L, Takeshita M, Johnson F, Iden C, Grollman AP (1981): Bleomycin-induced strand scission of DNA: Mechanism of deoxyribose cleavage. J Biol Chem 256:8608-8615.
8. Hsie AW (1987): The use of the hgprt versus gpt locus for quantitative mammalian cell mutagenesis. In Moore MM. DeMarini DM, de Serres JJ, Tindall KR (eds): "Banbury Report 28: Mammalian Cell Mutagenesis." Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, pp 37-46.
9. Hsie AW, Recio L, Katz KS, Lee CQ, Wagner M, Schenley RL (1986): Evidence for reactive oxygen species inducing mutations in mammalian cells. Proc Natl Acad Sci USA 83:9616-9620.
10. Levin DE, Hollstein M, Christman MF, Schwiers EA, Ames BN (1982): A new Salmonella tester strain (TA102) with A-T base pairs at the site of mutation detects oxidative mutagens. Proc Natl Acad Sci USA 79:7445-7449.
11. Mello-Filho AC, Meneghini R (1984): Cell killing and DNA damage by hydrogen peroxide are mediated by intracellular iron. Biochem J 218:273-275.
12. Povirk LF (1987): Bleomycin-induced mutagenesis in repackaged lambda phage: Base substitution hotspots at the sequence C-G-C-C. Mutat Res 180:1-9.
13. Povirk LF (1996): DNA damage and mutagenesis by radiomimetic DNA-cleaving agents: bleomycin, neocarzinostatin and other enediynes. Mutat Res 355:71-89.
14. Povirk LF, Austin MJF (1991): Genotoxicity of bleomycin. Mutat Res 257:127-143.
15. Povirk LF, Bennett RAO, Wang P, Swerdlow PS, Austin MJ (1994): Single base-pair deletions induced by bleomycin at potential double-strand cleavage sites in the aprt gene of stationary phase Chinese hamster ovary D422 cells. J Mol Biol 243:216-226.
16. Sanger F, Nicklen S, Aoulson AR (1977): DNA sequencing with the chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5473.
17. Rabow LE, McGall GH, Stubbe J, Kozaich JW (1990): Identification of the source of oxygen in the alkaline-labile product accompanying cytosine release during bleomycin-mediated oxidative degradation of d (CGCGCG). J Am Chem Soc 112:3203-3208.
18. Stankowski LF Jr, Tindall KR (1987): Characterization of the AS52 cell line for use in mammalian cell mutagenesis studies. In: Moore MM, DeMarini DM, de Serres FJ, Tindall KR (eds): "Banbury Report 28: Mammalian Cell Mutagenesis." Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, pp 71-79.
19. Tindall KR, Stankowski LF Jr (1987): Deletion mutations are associated with the differential-induced mutant frequency response of the AS52 and CHO-K1-BH4 cells. In: Moore MM, DeMarini DM, de Serres FJ, Tindall KR (eds): "Banbury Report 28: Mammalian Cell Mutagenesis." Cold Spring Harbor, NY: Cold Spring Harbor Laboratory, pp 283-292.
20. Tindall KR, Stankowski LF Jr (1989): Molecular analysis of spontaneous mutations at the gpt locus in Chinese hamster ovary (AS52) cells. Mutat Res 220:241-253.
21. Xu Z, Yu Y, Gibbs RA, Caskey CT, Hsie AW (1993): Multiplex DNA amplification and solid-phase direct sequencing for mutation analysis at the hprt locus in Chinese hamster cells. Mutat Res 288:237-248.
22. Xu Z, Yu Y, Schwartz JL, Meltz ML, Hsie AW (1995): Molecular nature of spontaneous mutations at the hypoxanthine-guanine phosphoribosyltransferase (hprt) locus in Chinese hamster ovary cells. Environ Mol Mutagen 26:127-138.
23. Yu Y (1992): Molecular analysis of Adriamycin-induced mutations at the hprt and the gpt loci in Chinese hamster cells. PhD Dissertation, the University of Texas Medical Branch, Galveston, Texas, p 190.
24. Yu Y, Xu Z, Gibbs RA, Hsie Aw (1992): Polymerase chain reaction-based comprehensive procedure for the analysis of the mutation spectrum at the hypoxanthine-guanine phosphoribosyltransferase locus in Chinese hamster cells. Environ Mol Mutagen 19:267-273.