Determination of insulin content in pancreatic β cell line MIN6 cells by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

Biological and Pharmaceutical Bulletin

Volumn 21, Issue 11, 1998, Pages 1240-1241

  Tamura, Atsushi ^a   Azuma, Tsuyoshi ^a   Kayo, Ikeda ^a   Ozawa, Koichiro ^a   Masujima, Tsutomu ^a  

^a HIROSHIMA UNIV SCHOOL OF MEDICINE   (Japan)

Author keywords

Intracellular insulin content; MALDI TOF MS; MIN6 cells

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL LEVEL; HORMONAL REGULATION; HORMONE DETERMINATION; HORMONE RESPONSE; INSULINOMA; IONIZATION; LASER; MASS SPECTROMETRY; MOUSE; NONHUMAN; PANCREAS ISLET BETA CELL;

EID: 0031796340     PISSN: 09186158     EISSN: None     Source Type: Journal    
DOI: 10.1248/bpb.21.1240     Document Type: Article

References (6)

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5. The MALDI mass spectrophotometer used to obtain the mass spectra was a Voyager-RP (PerSeptive Biosystems, Framingham, MA). The accelerating voltage used was 20 kV. The measurement of mass spectra was performed in a linear mode. Matrices used in this experiment were 3,5 dimethoxy-4-hydroxycinnamic acid and α-cyano-4-hydroxy cinnnamic acid.
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