Thin-alginate-layer technique for protoplast culture of tobacco leaf protoplasts: Shoot formation in less than two weeks

Protoplasma

Volumn 204, Issue 1-2, 1998, Pages 114-118

  Dovzhenko, A ^a   Bergen, U ^a   Koop, H U ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Nicotiana tabacum; Protoplast culture; Shoot regeneration; Thin alginate layer technique

Indexed keywords

PLANT CELL CULTURE; PROTOPLAST; REGENERATION;

NICOTIANA TABACUM;

EID: 0031796199     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF01282299     Document Type: Article

References (14)

1. Fujimura T, Sakurai M, Negishi T, Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Cult Lett 2: 74-75
2. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151-158
3. Gleba YY, Sidorov VA, Hoffman F (1984) Mechanical isolation and single-cell culture of isolated protoplasts and somatic hybrid cells. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic Press, Orlando, pp 405-414
4. Golds TJ, Babczinsky J, Rauscher G, Koop HU (1992) Computer-controlled tracking of single cell development in Nicotiana tabacum L. and Hordeum vulgare L. protoplasts embedded in agarose/alginate films. J Plant Physiol 140: 582-587
5. Hall RD, Riksen-Bruinsma T, Weyens G, Lefèbre M, Dunwell JM, van Tunen A, Krens FA (1997) Sugar beet guard cell protoplasts demonstrate a remarkable capacitiy for cell division enabling applications in stomatal physiology and molecular breeding. J Exp Bot 28: 255-263
6. Koop HU, Schweiger HG (1985) Regeneration of plants from individually cultivated protoplasts using an improved microculture system. J Plant Physiol 121: 245-257
7. _ Steinmüller K, Wagner H, R̈ßler C, Eibl C, Sacher L (1996) Integration of foreign sequences into the tobacco plastonie via polyethylene glycol-mediated protoplast transformation. Planta 199: 193-201
8. Kuchuk N, Herrrnann RG, Koop HU (1988) Plant regeneration from leaf protoplasts of evening primrose (Oenothera hookeri). Plant Cell Rep 17: 601-604
9. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497
10. Puonti-Kaerlas J, Eriksson T (1988) Improved protoplast culture and regeneration of shoots in pea (Pisum sativum L.). Plant Cell Rep 7: 242-245
11. Schäffler E, Koop HU (1990) Single cell nurse culture of tobacco protoplasts: physiological analysis of conditioning factors. J Plant Physiol 137: 95-101
12. Takebe I, Nagata T (1984) Isolation and culture of protoplasts: tobacco. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic Press, Orlando, pp 328-339
13. _ Labib G, Melchers G (1971) Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwissenschaften 58: 318-320
14. Vardi A, Spiegel-Roy P (1982) Plant regeneration from Citrus protoplasts: variability in methodological requirements among C. limon cultivars and species. Theor Appl Genet 62: 171-176