Disruption of mitochondrial activities in rabbit and human hepatocytes by a quinoxalinone anxiolytic and its carboxylic acid metabolite

Toxicology

Volumn 131, Issue 1, 1998, Pages 33-47

  Ulrich, Roger G ^a   Bacon, James A ^a   Cramer, Clay T ^a   Petrella, Diane K ^a   Sun, Elena L ^a   Meglasson, Martin D ^a   Holmuhamedov, Ekhson ^a  

^a PFIZER INC   (United States)

Author keywords

oxidation; Cyclopropane carboxylic acid; Human; Liver; Mitochondria; Panadiplon; Rabbit; Rhodamine 123

Indexed keywords

ADENOSINE TRIPHOSPHATE; ANXIOLYTIC AGENT; OXYGEN; PALMITIC ACID; PANADIPLON; QUINOXALINE DERIVATIVE; RHODAMINE 123;

ADULT; ANIMAL CELL; ARTICLE; CELL DEATH; CONTROLLED STUDY; FATTY ACID OXIDATION; HUMAN; HUMAN CELL; LIVER CELL; LIVER CELL CULTURE; LIVER MITOCHONDRION; LIVER TOXICITY; MALE; MITOCHONDRIAL RESPIRATION; NONHUMAN; OXYGEN CONSUMPTION; PRIORITY JOURNAL; RABBIT;

ADOLESCENT; ADULT; ANIMALS; ANTI-ANXIETY AGENTS; CELL RESPIRATION; CELLS, CULTURED; CHILD, PRESCHOOL; CYCLOPROPANES; FEMALE; FLUORESCENT DYES; HUMANS; LIVER; MALE; MIDDLE AGED; MITOCHONDRIA, LIVER; OXADIAZOLES; OXIDATION-REDUCTION; QUINOXALINES; RABBITS; RATS; RHODAMINE 123; SPECIES SPECIFICITY;

EID: 0031795059     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0300-483X(98)00118-8     Document Type: Article

References (40)

1. Agius L., Peak M., Sherratt S.A. Differences between human, rat, and guinea pig hepatocyte cultures: A comparative study of their rates of β-oxidation and esterification of palmitate and their sensitivity to R-etomoxir. Biochem. Pharm. 42:1991;1711-1715.
2. Bacon J.A., Cramer C.T., Petrella D.K., Sun E.L., Ulrich R.G. Potentiation of hypoxic injury in cultured rabbit hepatocytes by the quinoxalinone anxiolytic, panadiplon. Toxicology. 108:1996;9-16.
3. Bremer, J., Christiansen, R.Z., Borreback, B., 1978. Regulation of partition of free fatty acids between triglyceride synthesis and β-oxidation in liver. Reg. Mech. Carbohyd. Metab., V. Esmann: Pergamon Press, Oxford, pp. 161-170.
4. Buxton D.B., Bahl J.J., Olson M.S. The effects of cyclopropane carboxylic acid on hepatic pyruvate metabolism. Metabolism. 32:1983;736-744.
5. Chen L.B. Mitochondrial membrane potential in living cells. Ann. Rev. Cell Biol. 4:1988;155-182.
6. Chesne C., Guyomard C., Fautrel A., Poullain M.G., Fremond B., De Jong H., Guillouzo A. Viability and function in primary culture of adult hepatocytes from various animal species and human beings after cryopreservation. Hepatology. 18:1993;406-414.
7. Danan G., Trunet P., Bernuau J., Degott C., Babany G., Pessayer D., Rueff B., Benhamou J. Pirprofen-induced fulminant hepatitis. Gastroenterology. 89:1985;210-213.
8. Duncombe W.G., Rising T.J. Studies on the hypoglycaemic compound cyclopropane carboxylic acid: Effects on fatty acid oxidation in vitro. Biochem. Pharm. 21:1972;1075-1088.
9. Duncombe W.G., Rising T.J. Studies on the hypoglycaemic compound cyclopropane carboxylic acid. Effects on gluconeogenesis in vitro. Biochem. Pharm. 21:1972;1089-1096.
10. Ehrenberg B., Montana V., Wei M.D., Wuskell J.P., Loew L.M. Membrane potential can be determined in individual cells from the Nernstian distribution of cationic dyes. Biophys. J. 53:1988;785-794.
11. Elliget K.A., Kolaja G.J. Preparation of primary cultures of rat hepatocytes suitable for in vitro toxicity testing. J. Tissue Culture Methods. 8:1983;1-6.
12. Fromenty B., Pessayre D. Inhibition of mitochondrial beta-oxidation as a mechanism of hepatotoxicity. Pharmacol. Ther. 67:1995;101-154.
13. Geneve J., Hayat-Bonan B., Labbe G., Degott C., Letteron P., Freneaux E., Dinh T., Larrey D., Pessayre D. Inhibition of mitochondrial β-oxidation of fatty acids by pirprofen. Role in microvesicular steatosis due to this nonsteroidal anti-inflammatory drug. J. Pharm. Exp. Ther. 242:1987;1133-1137.
14. Hirsch J.D., Beyer C.F., Malkowitz L., Beer B., Blume A.J. Mitochondrial benzodiazepine receptors mediate inhibition of mitochondrial respiratory control. Mol. Pharm. 35:1989;157-163.
15. Johnson L.V., Walsh M.L., Chen L.B. Localization of mitochondria in living cells with rhodamine 123. Proc. Natl. Acad. Sci. USA. 77:1980;990-994.
16. Johnson L.V., Walsh M.L., Bockus B.J., Chen L.B. Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy. J. Cell Biol. 88:1981;526-532.
17. Kesterson J.W., Granneman G.R., Machinist J.M. The hepatotoxicity of valproic acid and its metabolites in rats. I. Toxicologic, biochemical and histopathologic studies. Hepatology. 6:1984;1143-1152.
18. Lowry O.H. Micromethods for assays of enzymes. Method. Enzymol. IV:1957;366-381.
19. Mayes P.A., Felts J.M. Regulation of fat metabolism in the liver. Nature. 215:1967;716-718.
20. Meglasson M.D., Smith K.M., Nelson D., Erecinska M. a-Glycerophosphate shuttle in a clonal b-cell line. Am. J. Physiol. 256:1989;E173-E178.
21. Melde K., Jackson S., Bartlett K., Sherratt H.S.A., Ghisla S. Metabolic consequences of methylenecyclopropylglycine poisoning in rats. Biochem. J. 274:1991;395-400.
22. Osterloh J., Cunningham W., Dixon A., Combest D. Biochemical relationships between Reye's and Reye's-like metabolic and toxicological syndromes. Med. Toxicol. Adverse Drug Exp. 4:1989;272-294.
23. Otto D.A., Chatzidakis C., Kasziba E., Cook G.A. Reciprocal effects of 5-(tetradecyloxy)-2-furoic acid on fatty acid oxidation. Arch. Biochem. Biophys. 242:1985;23-31.
24. Piercey M.F., Nielsen E.O., Honore T., Hoffmann W.E. Panadiplon is a benzodiazepine agonist in some brain sites and an antagonist in others: a 2-DG autoradiography study. Pharmacologist. 32:1990;136.
25. Rahn C.A., Bombick D.W., Doolittle D.J. Assessment of mitochondrial membrane potential as an indicator of cytotoxicity. Fundam. Appl. Toxicol. 16:1991;435-448.
26. Seglan P.O. Preparation of rat liver cells III. Enzymatic requirements for tissue dispersion. Exp. Cell Res. 82:1973;391-398.
27. Sherratt, S.A., 1986. Hypoglycin, the famous toxin of the unripe Jamaican ackee fruit. TIPS, May, pp. 186-191.
28. Speed W., Parton A.H., Martin I.J., Howard M.R. The use of liquid chromatography/thermospray mass spectrometry with on-line UV diode array and radiochemical detection: Characterisation of the putative metabolites of U-78875 in female rat faeces. Biol. Mass Spectrom. 23:1993;1-5.
29. Steenwyk, R.C., Sowell, C.G., Gammill, R.B., Pearson, P.G., 1994. Metabolic release of cyclopropane carboxylic acid from U-78875: Identification of cyclopropylcarbonylcarnitine by gas chromatography tandem mass spectrometry (GC-MS-MS). Proc. 42nd ASMS Conf. Mass Spec., 29 May-3 June, abstract 58.
30. Steinhelper M.E., Olson M.S. The effects of cyclopropanecarboxylate on hepatic pyruvate metabolism. Arch. Bioch. Biophys. 243:1985;80-91.
31. Strom S.C., Fisher R.A., Thompson M.T., Sanyal A.J., Cole P.E., Ham J.M., Posner M.P. Hepatocyte transplantation as a bridge to orthotopic liver transplantation in terminal liver failure. Transplantation. 63:1997;559-569.
32. Sun E.L., Aspar D.G., Ulrich R.G., Melchior G.W. Cryopreservation of cynomolgus monkey (Macaca fascicularis) hepatocytes for subsequent culture and protein synthesis studies. In Vitro Cell. Dev. Biol. 25:1990;147-150.
33. Tang A.H., Franklin S.R., Himes C.S., Ho P.M. Behavioral effects of U-78875, a quinoxalinone anxiolytic with potent benzodiazepine antagonist activity. J. Pharmacol. Exp. Ther. 259:1991;248-254.
34. Ulrich R.G., Aspar D.G., Cramer C.T., Kletzien R.F., Ginsberg L.G. Isolation and culture of hepatocytes from the cynomolgus monkey (Macaca fascicularis). In Vitro Cell. Dev. Biol. 26:1990;815-823.
35. Ulrich R.G., Petry T.W., Bacon J.A., Blakeman D.P., Cramer C.T., Jolly R.A., Petrella D.K., Sun E.L. Toxicity of a fatty acid analogue, cyclopropane carboxylic acid, in the Dutch-belted rabbit. Toxicologist. 13:1993;199.
36. Ulrich R.G., Bacon J.A., Branstetter D.G., Cramer C.T., Funk G.M., Hunt C.E., Petrella D.K., Sun E.L. Induction of an hepatic toxic syndrome in the Dutch-belted rabbit by a quinoxalinone anxiolytic. Toxicology. 98:1995;187-198.
37. Ulrich R.G., Cramer C.T., Sun E.L., Bacon J.A., Petrella D.K. A protocol for isolation, culture and cryopreservation of hepatocytes from human liver. In Vitro Mol. Toxicol. 11:1998;23-33.
38. VonVoigtlander P.F., Collin R.J., Watien F., Christensen A., Jensen L.H., Honore T. The discovery of panadiplon, a benzodiazepine antagonist anxiolytic. Pharmacologist. 32:1990;136.
39. Wroblewski F., LaDue J.S. Lactic dehydrogenase activity in blood. Proc. Soc. Exp. Biol. Med. 90:1955;210-213.
40. Zimmerman H.J., Kamal G.I. Valproate-induced hepatic injury: analyses of 23 fatal cases. Hepatology. 2:1982;591-597.