RNA isolation from human skin tissues for colorimetric differential display

Journal of Biochemical and Biophysical Methods

Volumn 37, Issue 3, 1998, Pages 131-135

  Hipfel, Rainer ^a   Garbe, Claus ^a   Schittek, Birgit ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

Author keywords

Colorimetric detection; Differential display; RNA isolation; Skin tissue

Indexed keywords

RIBONUCLEASE; RNA;

COLORIMETRY; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL; RNA ANALYSIS; SHORT SURVEY; SKIN; SKIN BIOPSY;

EID: 0031792012     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(98)00023-2     Document Type: Article

References (9)

1. Dobbeling U., Boni R., Haffner A., Dummer R., Burg G. Method for simultaneous RNA and DNA isolation from biopsy material, culture cells, plants and bacteria. Biotechniques. 22:1997;88-90.
2. Liang P., Pardee A.B. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction [see comments]. Science. 257:1992;967-971.
3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162:1987;156-159.
4. Sparmann G, Jaschke A, Loehr M, Liebe S, Emmrich J. Tissue homogenization as a key step in extracting RNA from human and rat pancreatic tissue. Biotechniques 1997;22:408-10412.
5. Lohmann J., Schickle H., Bosch T.C. REN display, a rapid and efficient method for nonradioactive differential display and mRNA isolation. Biotechniques. 18:1995;200-202.
6. Bauer D., Muller H., Reich J.et al. Identification of differentially expressed mRNA species by an improved display technique (DDRT-PCR). Nucleic Acids Res. 21:1993;4272-4280.
7. An G., Luo G., Veltri R.W., O'Hara S.M. Sensitive, nonradioactive differential display method using chemiluminescent detection. Biotechniques. 20:1996;342-346.
8. Rompf R, Kahl G. mRNA differential display in agarose gels. Biotechniques 1997;23:28,30,32.
9. Chen J.J., Peck K. Non-radioisotopic differential display method to directly visualize and amplify differential bands on nylon membrane. Nucleic Acids Res. 24:1996;793-794.