Detection of anti-PL-12 autoantibodies by ELISA using a recombinant antigen; study of the immunoreactive region

Clinical and Experimental Immunology

Volumn 114, Issue 2, 1998, Pages 161-165

  Garcia Lozano J R ^a   Gonzalez Escribano M F ^a   Rodriguez R ^a   Rodriguez Sanchez, J L ^b   Targoff, I N ^c   Wichmann, I ^a   Nunez Roldan A ^a  

^a HOSPITAL UNIVERSITARIO VIRGEN DEL ROCÍO   (Spain)

^b HOSPITAL DE LA SANTA CREU I SANT PAU   (Spain)

^c OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

Author keywords

Aminoacyl tRNA synthetase; Autoimmunity; ELISA; Expression cDNA; Library; PL 12 autoantibodies

Indexed keywords

ALANINE TRANSFER RNA LIGASE; AUTOANTIBODY; COMPLEMENTARY DNA; EPITOPE; RECOMBINANT PROTEIN;

ANTIBODY DETECTION; ARTICLE; AUTOIMMUNE DISEASE; CONTROLLED STUDY; DNA LIBRARY; DNA SEQUENCE; ENZYME ACTIVE SITE; ENZYME LINKED IMMUNOSORBENT ASSAY; HELA CELL; HUMAN; HUMAN CELL; IMMUNOREACTIVITY; MOLECULAR CLONING; MYOSITIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; SERUM;

ALANINE-TRNA LIGASE; AUTOANTIBODIES; AUTOANTIGENS; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; EPITOPES, B-LYMPHOCYTE; HELA CELLS; HUMANS; RECOMBINANT PROTEINS;

EID: 0031790050     PISSN: 00099104     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2249.1998.00720.x     Document Type: Article

References (21)

1. Reichlin M, Arnett FC Jr. Multiplicity of antibodies in myositis sera. Arthritis Rheum 1984; 27:1150-6.
2. Yoshida S, Akizuki M, Mimori T, Yamagata H, Inada S, Homma M. The precipitating antibody to an acidic nuclear protein antigen, the Jo-1, in connective tissue disease. A marker for a subset of polymyositis with interstitial pulmonary fibrosis. Arthritis Rheum 1983; 26:604-11.
3. Targoff IN, Plotz P. Autoantigens in myositis. In: Van Venrooij WJ, Maini RN, eds. Manual of biological markers of disease. Dordrecht: Kluwer Academic Publishers, 1996;C6.1:1-12.
4. ala coexist and are associated with myositis. J Exp Med 1986; 163:1281-91.
5. Targoff I. Aminoacyl-tRNA (other than histidyl) synthetase autoantibodies. In: Peter JB, Shoenfeld Y, eds. Autoantibodies. Amsterdam: Elsevier Science B.V., 1996:36-46.
6. Biswas T, Miller FW, Takagaki Y, Plotz PH. An enzyme-linked immunosorbent assay for the detection and quantitation of anti-Jo-1 antibody in human serum. J Immunol Methods 1987; 98:243-8.
7. Raben N, Nichols R, Dohlman J, McPhie P, Sridhar V, Hyde C, Leff R, Plotz P. A motif in human histidyl-tRNA synthetase which is shared among several aminoacyl-tRNA sinthetases is a coiled-coil that is essential for enzymatic activity and contains the major autoantigen epitope. J Biol Chem 1994; 269:24277-83.
8. Ge Q, Trieu EP, Targoff IN. Primary structure and functional expression of human glycyl-tRNA synthetase, an autoantigen in myositis. J Biol Chem 1994; 269:28790-7.
9. Florentz C, Briand JP, Giege R. Possible functional role of viral tRNA-like structures. FEBS 1984; 176:295-300.
10. Christensen ML, Pachmann LM, Schneiderman R, Patel DC, Friedman JM. Prevalence of Coxsackie B virus antibodies in patients with juvenile dermatomyositis. Arthritis Rheum 1986; 29:1365-70.
11. Cronin ME, Love LA, Miller FW, McClintock PR, Notkins AL, Plotz PH. The natural history of encephalomyocarditis virus-induced myositis and myocarditis in mice. Viral persistence demonstrated by in situ hybridization. J Exp Med 1988; 168:1639-48.
12. Ge Q, Wu Y, Targoff IN. Analysis of epitope reactivity of autoantibodies to glycyl-tRNA synthetase. Arthritis Rheum 1994; 37:S351.
13. Targoff IN, Arnett FC. Clinical manifestations in patients with antibody to PL-12 antigen (alanyl-tRNA synthetase). Am J Med 1990; 88:241-51.
14. Young RA, Davis RW. Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci USA 1983; 80:1194-8.
15. Garcia-Lozano R, Gonzalez-Escribano F, Sánchez-Roman J, Whichmann I, Nuñez-Roldan A. Presence of antibodies to different replication protein A in autoimmune sera. Proc Natl Acad Sci USA 1995; 92:5116-20.
16. Devereux J, Haeberli P, Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 1984; 12:387-95.
17. Charles PJ, Maini RN. Enzyme-linked immunosorbant assay in the rheumatological laboratory. In: Van Venrooij WJ, Maini RN, :eds. Manual of biological markers of disease. Dordrecht: Kluwer Academic Publishers, 1993; A5:1-23.
18. Lerner MR, Steitz JA. Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. Proc Natl Acad Sci USA 1979; 76:5495-9.
19. Shiba K, Ripmaster T, Suzuki N, Nichols R, Plotz P, Noda T, Schimmel P. Human alanyl-tRNA synthetase: conservation in evolution of catalytic core and microhelix recognition. Biochem 1995; 34:10340-9.
20. Miller FW, Waite KA, Biswas T, Plotz PH. The role of an autoantigen. histidyl-tRNA synthetase, in the induction and maintenance of autoimmunity. Proc Natl Acad Sci USA 1990; 87:9933-7.
21. Bunn CC, Mathews MB. Autoreactive epitope defined as the anticodon region of alanine transfer RNA. Science 1987; 238:1116-9.