Enhancement of cytotoxicity by electropermeabilization: An improved method for screening drugs

Anti-Cancer Drugs

Volumn 9, Issue 4, 1998, Pages 319-325

  Gehl, Julie ^a   Skovsgaard, Torben ^a   Mir, Lluis M ^b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b INSTITUT GUSTAVE ROUSSY   (France)

Author keywords

Cytotoxicity; Electrochemotherapy; Electroper meabilization; Electroporation; Lucifer yellow; MTT

Indexed keywords

ANTINEOPLASTIC AGENT; BLEOMYCIN; CARBOPLATIN; CISPLATIN; DAUNORUBICIN; DOXORUBICIN; ETOPOSIDE; PACLITAXEL;

ANIMAL CELL; ARTICLE; CELL MEMBRANE PERMEABILITY; CONTROLLED STUDY; CYTOTOXICITY; CYTOTOXICITY TEST; DRUG SCREENING; ELECTROCHEMOTHERAPY; ELECTROPORATION; FLOW CYTOMETRY; NONHUMAN; PRIORITY JOURNAL;

EID: 0031779576     PISSN: 09594973     EISSN: None     Source Type: Journal    
DOI: 10.1097/00001813-199804000-00005     Document Type: Article

References (22)

1. Orlowski S, Mir LM. Cell electropermeabilization: a new tool for biochemical and pharmacological studies. Biochim Biophys Acta 1993; 1154: 51-63.
2. Potter H. Electroporation in biology: methods, applications, and instrumentation. Anal Biochem 1988; 174: 361-73.
3. Mir LM, Orlowski S, Belehradek J, Jr, Paoletti C. Electrochemotherapy potentiation of antitumour effect of bleomycin by local electric pulses. Eur J Cancer 1991; 27: 68-72.
4. Jaroszeski MJ, Gilbert RA, Heller R. In vivo antitumor effects of electrochemotherapy in a hepatoma model. Biochim Biophys Acta 1997; 1334: 15-8.
5. Salford LG, Persson BR, Brun A, Ceberg CP, Kongstad PC, Mir LM. A new brain tumour therapy combining bleomycin with in vivo electropermeabilization. Biochem Biophys Res Commun 1993; 194: 938-43.
6. Belehradek J, Jr, Orlowski S, Poddevin B, Paoletti C, Mir LM. Electrochemotherapy of spontaneous mammary tumours in mice. Eur J Cancer 1991; 27: 73-6.
7. Serša G, Čemažar M, Miklavčič D, Mir LM. Electrochemotherapy: variable anti-tumor effect on different tumor models. Bioelectrochem Bioenerget 1994 35: 23-7.
8. Heller R, Jaroszeski M, Leo-Messina J, et al. Treatment of B16 mouse melanoma with the combination of electropermeabilization and chemotherapy. Bioelectrochem Bioenerget 1995; 36: 83-7.
9. Serša G, Cemazar M, Miklavcic D. Antitumor effectiveness of electrochemotherapy with cis-diamminedichloroplatinum(II) in mice. Cancer Res 1995; 55: 3450-5.
10. Belehradek M, Domenge C, Luboinski B, Orlowski S, Belehradek J, Jr, Mir LM. Electrochemotherapy, a new antitumor treatment. First clinical phase I-II trial. Cancer 1993; 72: 3694-700.
11. Domenge C, Orlowski S, Luboinski B, et al. Antitumor electrochemotherapy: new advances in the clinical protocol. Cancer 1996; 77: 956-63.
12. Heller R, Jaroszeski MJ, Glass LF, et al. Phase I/II trial for the treatment of cutaneous and subcutaneous tumors using electrochemotherapy. Cancer 1996; 77: 964-71.
13. Mir LM, Glass LF, Serša G, et al. Effective treatment of cutaneous and subcutaneous malignant tumors by electrochemotherapy. Br J Cancer 1998; in press.
14. Orlowski S, Belehradek J, Jr, Paoletti C, Mir LM. Transient electropermeabilization of cells in culture. Increase of the cytotoxicity of anticancer drugs. Biochem Pharmacol 1988; 37: 4727-33.
15. Mosmann T. Rapid colorimetric assay for cellular growth and cytotoxicity assays. J Immunol Methods 1983; 65: 55-63.
16. Tada H, Shiho O, Kuroshima K, Koyama M, Tsukamoto K. An improved colorimetric assay for interleukin 2. J Immunol Methods 1986; 93: 157-65.
17. Poddevin B, Orlowski S, Belehradek J, Jr, Mir LM. Very high cytotoxicity of bleomycin introduced into the cytosol of cells in culture. Biochem Pharmacol 1991; 42 (suppl): S67-75.
18. Mir LM, Banoun H, Paoletti C. Introduction of definite amounts of nonpermeant molecules into living cells after electropermeabilization: direct access to the cytosol. Exp Cell Res 1988; 175: 15-25.
19. Dinchuk JE, Kelley KA, Callahan GN. Flow cytometric analysis of transport activity in lymphocytes electroporated with a fluorescent organic anion dye. J Immunol Methods 1992; 155: 257-65.
20. Belehradek J, Jr, Orlowski S, Ramirez LH, Pron G, Poddevin B, Mir LM. Electropermeabilization of cells in tissues assessed by the qualitative and quantitative electroloading of bleomycin. Biochim Biophys Acta 1994; 1190: 155-63.
21. Melvik JE, Pettersen EO, Gordon PB, Seglen PO. Increase in cis-dichlorodiammineplatinum (II) cytotoxicity upon reversible electropermeabilization of the plasma membrane in cultured human NHK 3025 cells. Eur J Cancer Clin Oncol 1986; 22: 1523-30.
22. Skovsgaard T. Transport and binding of daunorubicin, adriamycin, and rubidazone in Ehrlich ascites tumour cells. Biochem Pharmacol 1977; 26: 215-22.