Synergism between toxin-γ from Brazilian scorpion Tityus serrulatus and veratridine in chromaffin cells

American Journal of Physiology - Cell Physiology

Volumn 274, Issue 6 43-6, 1998, Pages

  Conceiçao, Isaltino M ^a,b   Lebrun, Ivo ^a   Cano Abad, María ^c   Gandía, Luis ^c   Hernández Guijo, Jesús M ^c   López, Manuela G ^c   Villarroya, Mercedes ^c   Jurkiewicz, Aron ^b   García, Antonio G ^c,d  

^a INSTITUTO BUTANTAN   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^c UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^d HOSPITAL UNIVERSITARIO DE LA PRINCESA   (Spain)

Author keywords

Calcium uptake; Catecholamine release; Oscillations of cytosolic calcium; Sodium current

Indexed keywords

TOXIN; VERATRIDINE;

ANIMAL CELL; ARTICLE; CALCIUM CELL LEVEL; CALCIUM TRANSPORT; CATECHOLAMINE RELEASE; CELL SECRETION; CHROMAFFIN CELL; CONCENTRATION RESPONSE; DOSE TIME EFFECT RELATION; NONHUMAN; OSCILLATORY POTENTIAL; PRIORITY JOURNAL; SCORPION; SODIUM CELL LEVEL; SODIUM CURRENT;

ANIMALIA; BOVINAE; INVERTEBRATA; SCORPIONES; TITYUS SERRULATUS;

EID: 0031776510     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.1998.274.6.c1745     Document Type: Article

References (40)

1. Almers, W., and E. Neher. The Ca signal from fura 2 loaded mast cells depends strongly on the method of dye-loading. FEBS Lett. 192: 13-18, 1985.
2. + channel. J. Biol. Chem. 257: 12553-12558, 1982.
3. + channel in muscle, with a selectivity for channels in the surface membrane. Pflügers Arch. 400: 22-27, 1984.
4. + channel from rat brain. EMBO J. 2: 915-920, 1982.
5. Borges, R., F. Sala, and A. G. García. Continuous monitoring of catecholamine release from perfused cat adrenal glands. J. Neurosci. Methods 16: 389-400, 1986.
6. Calvo, S., R. Granja, C. González-García, and V. Ceña. Catecholamine secretion, calcium levels and calcium influx in response to membrane depolarization in bovine chromaffin cells. Neuroscience 68: 265-272, 1995.
7. Carvalho, F. C., A. L. A. Nencioni, I. Lebrunj, V. A. C. Dorce, and M. R. L. Sandoval. Lesao hipocampal e actividades epileptica induzida por toxinas de veneno escorpionico. Abstr. Congr. Fed. Braz. Soc. Biol. Sci. IXth Caxambu Brazil p. 29, 1994.
8. Carvalho, F. F., A. L. A. Nencioni, I. Lebrun, M. R. L. Sandoval, and V. A. C. Dorce. Behavioral, electroencephalographic and histopathologic effects of a neuropeptide isolated from Tityus serrulatus scorpion venon in rats. Pharmacol. Biochem. Behav. In press.
9. Catterall, W. A. Structure and function of voltage-sensitive ion channels. Science 242: 50-61, 1988.
10. Caterall, W. A. Molecular properties of voltage-sensitive sodium channels. Annu. Rev. Biochem. 55: 953-985, 1996.
11. Ceña, V., G. P. Nicolás, P. Sánchez-García, S. M. Kirpekar, and A. G. García. Pharmacological dissection of receptor associated and voltage-sensitive ionic channels involved in catecholamine release. Neuroscience 10: 1455-1462, 1983.
12. Conceiçao, I., M., L. Gárcez-Do Carmo, R. Frussa-Filho, V. A. C. Dorce, M. R. L. Sandoval, I. Lebrun, and A. Jurkiewicz. Efeitos da toxina II-4 do escorpiao Tityus serrulatus sobre a contratilidade do ducto deferente de rato. Abstr. Congr. Fed. Braz. Soc. Biol. Sci. IXth Caxambu Brazil p. 282, 1996.
13. Couraud, F., and E. Jover. Mechanism of action of scorpion toxins. In: Handbook of Natural Toxins, edited by A. T. Tu. New York: Dekker, 1984, vol. 2, p. 659-678.
14. Douglas, W. W., and R. P. Rubin. The role of calcium in the secretory responses of the adrenal medulla to acetylcholine. J. Physiol. (Lond.) 151: 40-57, 1961.
15. Fenwick, E. M., A. Marty, and E. Neher. Sodium and calcium channels in bovine chromaffin cells. J. Physiol. (Lond.) 331: 599-635, 1982.
16. Frankenhaeuser, B., and A. G. Hodgkin. The action of calcium on the electrical properties of squid axons. J. Physiol. (Lond.) 137: 218-244, 1957.
17. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260: 3440-3450, 1985.
18. Hamill, O. P., A. Marty, E. Neher, B. Sakmann, and F. J. Sigworth. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch. 391: 85-100, 1981.
19. + channels in the release of catecholamines from perfused adrenal glands. Br. J. Pharmacol. 62: 359-361, 1978.
20. Ito, S., Y. Nakazato, and A. Ohga. The effect of veratridine on the release of catecholamines from the perfused adrenal gland. Br. J. Pharmacol. 65: 319-330, 1979.
21. Ito, S., Y. Nakazato, and A. Ohga. Exocytotic release of catecholamines from perfused adrenal glands of guinea-pig induced by veratridine. Br. J. Pharmacol. 70: 527-535, 1980.
22. Jonas, P., W. Vogel, E. C. Arantes, and J. R. Giglio. Toxin γ of the scorpion Tityus serrulatus modifies both activation and inactivation of sodium permeability of nerve membrane. Pflügers Arch. 407: 92-99, 1986.
23. Kidokoro, Y., S. Miyazaki, and S. Ozawa. Acetylcholine-induced membrane depolarization and potential fluctuations in the rat adrenal chromaffin cell. J. Physiol. (Lond.) 324: 203-220, 1982.
24. Kidokoro, Y., and A. K. Ritchie. Chromaffin cell action potentials and their possible role in adrenaline secretion from rat adrenal medulla. J. Physiol. (Lond.) 307: 199-216, 1980.
25. Kilpatrick, D. L., R. Slepetis, and N. Kirshner. Ion channels and membrane potential in stimulus-secretion coupling in adrenal medulla cells. J. Neurochem. 36: 1245-1255, 1981.
26. Kirpekar, S. M., and J. C. Prat. Release of catecholamines from perfused cat adrenal gland by veratridine. Proc. Natl. Acad. Sci. USA 76: 2081-2083, 1979.
27. Knight, D. E., and M. J. Whitaker. Veratridine-induced secretion in medullary cells isolated from the bovine adrenal gland (Abstract). J. Physiol. (Lond.) 281: 18P-19P, 1978.
28. 28a. Lima, M. E., and M. F. Martin-Euclair. The toxins purified from Tityus serrulatus (Lutz and Mello) venom. J. Toxicol. Toxin Rev. 14: 457-481, 1995.
29. Livett, B. G. Adrenal medullary chromaffin cells in vitro. Physiol. Rev. 64: 1103-1161, 1984.
30. López, M. G., A. R. Artalejo, A. G. García, E. Neher, and J. García-Sancho. Veratridine-induced oscillations of cytosolic calcium and membrane potential in bovine chromaffin cells. J. Physiol. (Lond.) 482: 15-27, 1995.
31. 2+ oscillations induced by veratridine. Eur. J. Pharmacol. 270: 331-339, 1994.
32. Moro, M. A., A. G. García, and O. K. Langley. Characterization of two chromaffin cell populations isolated from bovine adrenal medulla. J. Neurochem. 57: 63-69, 1991.
33. Moro, M. A., M. G. López, L. Gandía, P. Michelena, and A. G. García. Preparation and culture of living epinephrine- and norepinephrine-containing cells from bovine adrenal medullae. Anal. Biochem. 185: 243-248, 1990.
34. Nassar-Gentina, V., H. B. Pollard, and E. Rojas. Electrical activity in chromaffin cells of intact mouse adrenal gland. Am. J. Physiol. 254 (Cell Physiol. 23): C675-C683, 1988.
35. Ohta, M., T. Narahashi, and R. F. Keeler. Effects of veratrum alkaloids on membrane potential and conductance of squid and cray fish giant axons. J. Pharmacol. Exp. Ther. 184: 143-154, 1973.
36. Possani, L. D., A. C. Alagon, P. L. Fletcher, Jr., and B. M. Erickson. Purification and properties of mammalian toxins from the venom of Brazilian scorpion Tityus serrulatus Lutz and Mello. Arch. Biochem. Biophys. 180: 394-403, 1977.
37. 2IV from Tityus serrulatus scorpion venom. Toxicon 29: 663-672, 1991.
38. Sampaio, S. V., C. J. Laure, and J. R. Giglio. Isolation and characterization of toxic proteins from the venom of the Brazilian scorpion Tityus serrulatus. Toxicon 21: 265-277, 1983.
39. + channels in neuroblastoma cells. Pflügers Arch. 401: 297-303, 1984.
40. Yatani, A., G. E. Kirsch, L. D. Possani, and A. M. Brown. Effects of New World scorpion toxins on single-channel and whole cell cardiac sodium currents. Am. J. Physiol. 254 (Heart Circ. Physiol. 23): H443-H451, 1988.