Evidence for separate effects of U73122 on phospholipase C and calcium channels in human platelets

Biochemical Pharmacology

Volumn 56, Issue 11, 1998, Pages 1481-1484

  Pulcinelli, Fabio M ^a   Gresele, Paolo ^b   Bonuglia, Margherita ^a   Gazzaniga, Pier Paolo ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b UNIVERSITY OF PERUGIA   (Italy)

Author keywords

Calcium channels; Calcium mobilization; Phospholipase C; Platelets

Indexed keywords

1 [[6 (3 METHOXYESTRA 1,3,5(10) TRIEN 17BETA YL)AMINO]HEXYL] 1H PYRROLE 2,5 DIONE; 15 HYDROXY 11ALPHA,9ALPHA EPOXYMETHANOPROSTA 5,13 DIENOIC ACID; CALCIUM CHANNEL; CALCIUM ION; INOSITOL 1,4,5 TRISPHOSPHATE; MANGANESE; PHOSPHOLIPASE C; THROMBIN;

ARTICLE; CALCIUM CELL LEVEL; CONTROLLED STUDY; ENZYME ACTIVATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; THROMBOCYTE; THROMBOCYTE AGGREGATION INHIBITION; THROMBOCYTE MEMBRANE;

15-HYDROXY-11 ALPHA,9 ALPHA-(EPOXYMETHANO)PROSTA-5,13-DIENOIC ACID; BLOOD PLATELETS; CALCIUM; CALCIUM CHANNELS; CYTOSOL; ENZYME ACTIVATION; ESTRENES; HUMANS; INOSITOL 1,4,5-TRISPHOSPHATE; MANGANESE; PHOSPHODIESTERASE INHIBITORS; PHOSPHOLIPASE C; PLATELET AGGREGATION; PLATELET AGGREGATION INHIBITORS; PYRROLIDINONES;

EID: 0031770306     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(98)00146-4     Document Type: Article

References (16)

1. 2 in the nitric oxide-independent vasodilator effect of bradykinin in the rat perfused heart. J Pharmacol Exp Ther. 278:1996;518-526.
2. 2 mediates the stimulation of inositol 1,4,5-trisphosphate production and intracellular calcium mobilization by bradykinin in neonatal rat ventricular cardiomyocytes. Hypertension. 28:1996;444-449.
3. 2+/calmodulin-sensitive tyrosine kinase. J Biol Chem. 271:1996;14169-14175.
4. Smith R.J., Sam L.M., Justen J.M., Bundy G.L., Bala G.A., Bleasdale J.E. Receptor-coupled signal transduction in human polymorphonuclear neutrophils Effects of a novel inhibitor of phospholipase C-dependent processes on cell responsiveness . J Pharmacol Exp Ther. 253:1990;688-697.
5. 2+ signaling mechanisms of the β2 integrin on neutrophils involvement of phospholipase Cγ2 and Ins(1,4,5)P3 . Biochem J. 317:(Pt 2):1996;403-409.
6. 2+ release from intracellular stores evoked by α1a- and α2a-adrenoceptors in rat portal vein myocytes. Biochem Biophys Res Commun. 218:1996;30-34.
7. 2+ inflow. Biochem Pharmacol. 49:1995;1373-1379.
8. Grierson J.P., Meldolesi J. Calcium homeostasis in mouse fibroblast cells affected by U-73122 a putative phospholipase C β blocker, via multiple mechanisms . Br J Pharmacol. 115:1995;11-14.
9. Bleasdale J.E., Thakur N.R., Gremban R.S., Bundy G.L., Fitzpatrick F.A., Smith R.J., Bunting S. Selective inhibition of receptor-coupled phospholipase C-dependent processes in human platelets and polymorphonuclear neutrophils. J Pharmacol Exp Ther. 255:1990;756-768.
10. Heemskerk J.W., Farndale R.W., Sage S.O. Effects of U73122 and U73343 on human platelet calcium signalling and protein tyrosine phosphorylation. Biochim Biophys Acta. 1355:1997;81-88.
11. Aster R.H., Jandel J.H. Platelet sequestration in man. I. Methods. J Clin Invest. 43:1964;834-855.
12. Born G.V.R. The aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 194:1962;927-929.
13. 2+ indicators with greatly improved fluorescence properties. J Biol Chem. 260:1985;3440-3450.
14. 2+ and other ions. Watson S.P., Authi K.S. Platelets. A Practical Approach. 1996;67-90 IRL PRESS, Oxford-New York-Tokyo.
15. 32P-labeled inositol phosphates. J Chromatogr. 575:1992;51-55.
16. 3 . Thromb Haemost. 78:1997;220-225.