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2
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0031061411
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Pyrolobus fumarii, gen. and sp. nov., represents a novel group of archaea, extending the upper temperature limit of life to 113°C
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of outstanding interest. The isolation of the current record holder for high-temperature growth, Pyrolobus fumarii, is reported. Isolated from the Mid-Atlantic Ridge, the archaeon grows at temperatures up to 113°C. The archaeon is a facultatively aerobic obligate chemolithoautotroph, using hydrogen as an electron donor and nitrate thiosulfate and oxygen as terminal electron acceptors. The lipid side chains were predominantly tetraethers. Exponentially growing cultures of Pyrolobus fumarii were able to survive autoclaving for one hour at 121°C.
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Blochl E, Reinhard R, Burgraff S, Hafenbradl D, Jannasch HW, Stetter KO. Pyrolobus fumarii, gen. and sp. nov., represents a novel group of archaea, extending the upper temperature limit of life to 113°C. of outstanding interest Extremophiles. 1:1997;14-21 The isolation of the current record holder for high-temperature growth, Pyrolobus fumarii, is reported. Isolated from the Mid-Atlantic Ridge, the archaeon grows at temperatures up to 113°C. The archaeon is a facultatively aerobic obligate chemolithoautotroph, using hydrogen as an electron donor and nitrate thiosulfate and oxygen as terminal electron acceptors. The lipid side chains were predominantly tetraethers. Exponentially growing cultures of Pyrolobus fumarii were able to survive autoclaving for one hour at 121°C.
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of outstanding interest. A model for the universal ancestor of all extant life is presented. This model takes into account the most recent data accumulated from whole-genome sequences and the contradictory results of phylogenies derived from different genes. The 'genetic annealing' model is presented - a metaphor for its similarity to physical DNA annealing - that invokes the predominance of lateral over vertical gene transfer to explain the apparent chimeric features of present-day genomes.
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of special interest. Evidence is presented that two different groups of uncultivated marine archaea (Group I and Group II) occupy different zones in the water column. Both rRNA relative abudance and group-specific clones recovery reflected similar spatial distributions. Group II was highest in relative abundance at the surface, whereas Group I reached a maximum relative abudance below 100 m.
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Massana R, Murray AE, Preston CM, DeLong EF. Vertical distribution and phylogenetic characterization of marine planktonic Archaea in the Santa Barbara Channel. of special interest Appl Environ Microbiol. 63:1997;50-56 Evidence is presented that two different groups of uncultivated marine archaea (Group I and Group II) occupy different zones in the water column. Both rRNA relative abudance and group-specific clones recovery reflected similar spatial distributions. Group II was highest in relative abundance at the surface, whereas Group I reached a maximum relative abudance below 100 m.
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Massana, R.1
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Seasonal and spatial variability of bacterial and archaeal assemblages in the coastal waters off Anvers Island, Antarctica
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of special interest. A high abundance of Group I crenarchaeotes in Antarctic surface waters of ~1.8°C is confirmed. Seasonal variability in Antarctic marine archaea in surface waters was observed, with maxima occurring in late winter. An inverse relationship between chlorophyll concentration and archaeal abundance in Antarctic surface waters was observed.
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Murray AE, Preston CM, Massana R, Taylor LT, Blakis A, Wu K, DeLong EF. Seasonal and spatial variability of bacterial and archaeal assemblages in the coastal waters off Anvers Island, Antarctica. of special interest Appl Environ Microbiol. 64:1998;2585-2595 A high abundance of Group I crenarchaeotes in Antarctic surface waters of ~1.8°C is confirmed. Seasonal variability in Antarctic marine archaea in surface waters was observed, with maxima occurring in late winter. An inverse relationship between chlorophyll concentration and archaeal abundance in Antarctic surface waters was observed.
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Phylogenetic diversity of Archaea in sediment samples from a coastal salt marsh
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of special interest. This PCR-based survey of archaeal groups associated with marine sediments revealed the presence of close relatives of some of the cultivated archaeal types (e.g. methanogens and halophiles), as well as archaeal groups that are as yet uncultivated. Among the uncultivated groups, marine Group I and Group II archaea were detected. In addition, a new group peripherally related to Group II (labeled "Group III" in Figure 1) was also detected.
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Munson MA, Nedwell DB, Embley TM. Phylogenetic diversity of Archaea in sediment samples from a coastal salt marsh. of special interest Appl Environ Microbiol. 63:1997;4729-4733 This PCR-based survey of archaeal groups associated with marine sediments revealed the presence of close relatives of some of the cultivated archaeal types (e.g. methanogens and halophiles), as well as archaeal groups that are as yet uncultivated. Among the uncultivated groups, marine Group I and Group II archaea were detected. In addition, a new group peripherally related to Group II (labeled "Group III" in Figure 1) was also detected.
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Characterization of a DNA polymerase from the uncultivated psychrophilic archaeon Cenarchaeum symbiosum
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of outstanding interest. A DNA polymerase from an uncultivated, psychrophilic crenarchaeote (Cenarchaeum symbiosum) is cloned, sequenced and expressed. Although most similar in sequence to homologues from the cultivated hyperthermophilic crenarchaeotes, the C. symbiosum DNA polymerase was not thermostable. It was stable to temperatures of ~40°C, whereas related hyperthermophile DNA polymerases are stable at and above 80°C. This provided further evidence for a nonthermophilic phenotype, previously inferred solely from ecological distribution.
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Schleper C, Swanson RV, Mathur EJ, DeLong EF. Characterization of a DNA polymerase from the uncultivated psychrophilic archaeon Cenarchaeum symbiosum. of outstanding interest J Bacteriol. 79:1997;7803-7811 A DNA polymerase from an uncultivated, psychrophilic crenarchaeote (Cenarchaeum symbiosum) is cloned, sequenced and expressed. Although most similar in sequence to homologues from the cultivated hyperthermophilic crenarchaeotes, the C. symbiosum DNA polymerase was not thermostable. It was stable to temperatures of ~40°C, whereas related hyperthermophile DNA polymerases are stable at and above 80°C. This provided further evidence for a nonthermophilic phenotype, previously inferred solely from ecological distribution.
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of special interest. First report of cyclic and acyclic tetraether lipids associated with particulate material in marine plankton. Although the types of archaea contributing these lipids were not identified, the tetraethers were found in a variety of aerobic as well as anoxic water samples and sediments. The stable isotope signature of the lipids indicated an isotopically heavy carbon source.
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Hoefs MJL, Scouten S, de Leeuw JW, King LL, Wakeham SG, Damsté JSS. Ether lipids of planktonic archaea in the marine water column. of special interest Appl Environ Microbiol. 63:1997;3090-3095 First report of cyclic and acyclic tetraether lipids associated with particulate material in marine plankton. Although the types of archaea contributing these lipids were not identified, the tetraethers were found in a variety of aerobic as well as anoxic water samples and sediments. The stable isotope signature of the lipids indicated an isotopically heavy carbon source.
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Appl Environ Microbiol
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Hoefs, M.J.L.1
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40
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DeLong EF, King LL, Massana R, Cittone H, Murray A, Schleper C, Wakeham SG. Dibiphytanyl ether lipids in nonthermophilic crenarchaeotes. of special interest Appl Environ Microbiol. 64:1998;1133-1138 Confirmation that dibiphytanyl (tetraether) lipids are present in the uncultivated Group I marine crenarchaeotes. Disparate samples demonstrated to contain high numbers of the uncultivated archaea and enriched cell preparations of Cenarchaeum symbiousm were examined. All the archaea-containing samples derived from cold environments contained cyclic and acyclic tetraether lipids, identical to those of hyperthermophilic crenarchaeotes.
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Appl Environ Microbiol
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Delong, E.F.1
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