Immunochemical characterization of hepatic cytochrome P450 isozymes in the channel catfish: Assessment of sexual, developmental and treatment-related effects

Comparative Biochemistry and Physiology - C Pharmacology Toxicology and Endocrinology

Volumn 121, Issue 1-3, 1998, Pages 305-310

  Perkins, Everett J ^a   Schlenk, Daniel ^a  

^a NONE

Author keywords

Naphthoflavone; Channel catfish; Clofibric acid; Cytochrome P450; Developmental differences; Ethanol; Sex differences

Indexed keywords

ALCOHOL; BETA NAPHTHOFLAVONE; CLOFIBRIC ACID; CYTOCHROME P450 ISOENZYME; LIVER ENZYME;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CATFISH; CONTROLLED STUDY; IMMUNOCHEMISTRY; LIVER; NONHUMAN; PRIORITY JOURNAL; SEX DIFFERENCE; WESTERN BLOTTING;

ANIMALS; ANTIBODY SPECIFICITY; BETA-NAPHTHOFLAVONE; BLOTTING, WESTERN; CLOFIBRIC ACID; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME INDUCTION; ETHANOL; ICTALURIDAE; ISOENZYMES; MALE; MICROSOMES, LIVER; SEX FACTORS;

EID: 0031768847     PISSN: 07428413     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0742-8413(98)10051-8     Document Type: Article

References (27)

1. Andersson T, Forlin L Regulation of the cytochrome P450 enzyme system in fish. Aquat Toxicol. 24:1992;1-20.
2. Bradford MM A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:1976;248-254.
3. Bucheli TD, Fent K Induction of cytochrome P450 as a biomarker for environmental contamination in aquatic ecosystems. Crit Rev Environ Sci Technol. 25:1995;201-268.
4. Calender M, Buhler DR, Forlin L, Goksøyr A, Miranda CL, Woodin BR, Stegeman JJ Immunochemical relationships of cytochrome P4503A-like proteins in teleost fish. Fish Physiol Biochem. 15:1996;323-332.
5. Elskus AA, Pruell R, Stegeman JJ Endogenously-mediated, pretranslational suppression of cytochrome P450A in PCB-contaminated flounder. Mar Environ Res. 34:1992;97-101.
6. Gray ES, Woodin BR, Stegeman JJ Sex differences in hepatic monooxygenases in winter flounder (Pseudopleuronectus americanus) and scup (Stenotomus chrysops) and regulation of P450 forms by estradiol. J Exp Zool. 259:1991;330-342.
7. Haasch ML Induction of anti-trout lauric acid hydroxylase immunoreactive proteins by peroxisome proliferators in bluegill and catfish. Mar Environ Res. 42:1996;287-291.
8. Kaplan LAE, Schultz ME, Schultzt RJ, Crivello JF Nitrosodiethylamine metabolism in the viviparous fish Poeciliopsis: evidence for the existence of liver P450pj activity and expression. Carcinogenesis. 12:1991;647-652.
9. Kleinow KM, Haasch ML, Williams DE, Lech JJ A comparison of hepatic P450 induction in rat and trout (Oncorhynchus mykiss): delineation of the site of resistance of fish to phenobarbital-type inducers. Comp Biochem Physiol. 96C:1990;259-270.
10. Laemmli UK Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
11. Miranda CL, Wang J-L, Henderson MC, Zhao X, Guengerich FP, Buhler DR Comparison of rainbow trout and mammalian cytochrome P450 enzymes: evidence for structural similarity between trout P450 LMC5 and human P450IIIA4. Biochem Biophys Res Commun. 176:1991;558-563.
12. Miranda CL, Wang J, Henderson MC, Buhler DR Purification and characterization of hepatic steroid hydroxylase from untreated rainbow trout. Arch Biochem Biophys. 268:1989;227-238.
13. Monod G, Boudry M, Gillet C Biotransformation enzymes and their induction by β-naphthoflavone during embyolarval development an salmonid species. Comp Biochem Physiol C. 114C:1996;45-50.
14. Nelson DR, Koymans Luc, Kamatake Tetsuya P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics. 6:1996;1-42.
15. Roy NK, Kreamer G, Konkle B, Grunwald C, Wirgin I Characterization and prevelance of a polymorphism in the 3′ untranslated region of tomcod P45o1A1 in cacer-prone Atlantic tomcod. Arch Biochem Biophys. 322(2):1995;204-213.
16. Schlenk D, Ronis MJ, Miranda CL, Buhler DR Channel catfish liver monooxygenases: immunological characterization of constitutive cytochromes P450 and the absence of active flavin-containing monooxygenases. Biochem Pharmacol. 45:1993;217-221.
17. Schlenk D, Ronis MJJ, Miranda C, Buhler DR Effects of 2-methylisoborneol (MIB) and ethanol on the expression and activity of cytochrome P450s from the channel catfish. J Fish Biol. 46:1995;282-291.
18. Schlenk D, Buhler DR Role of flavin-containing monooxygenase in the in vitro biotransformation of aldicarb in Rainbow trout (Oncorhynchus mykiss). Xenobiotica. 21:1991;1583-1589.
19. Schlenk D Use of aquatic organisms as models to determine the in vivo contribution of flavin-containing monooxygenases in xenobiotic biotransformation. Mol Mar Biol Biotechnol. 4:1995;323-330.
20. Shields PG Pharmacogenetics: detecting sensitive populations. Env Health Perspect. 102:1994;81-87.
21. Smith CAD, Smith G, Wolf CR Genetic polymorphisms in xenobiotic metabolism. Eur J Cancer. 30A:1994;1921-1935.
22. Stegeman JJ, Hahn ME Biochemistry and molecular biology of monooxygenases: current perspectives on forms, functions, and regulation of cytochrome P450 in aquatic species. Malins DC, Ostrander GK Aquatic Toxicology: Molecular, Biochemical and Cellular Perspectives. 1st ed:1994;87-206 CRC Press, Boca Raton.
23. Stegeman JJ Cytochrome P450 forms in fish: catalytic, immunological and sequence similarities. Xenobiotica. 19:1989;1093-1110.
24. Stegeman JJ The cytochromes P450 in fish. Hochachka PW, Mommsen TP Molecular Biology Frontiers. 1993;137-156 Elsevier Science, Amsterdam.
25. Towbin H, Staehelin T, Gordon J Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA. 76:1979;4350-4354.
26. Williams DE, Buhler DR Purification of cytochromes P-448 from β-naphthoflavone-treated rainbow trout. Biochim Biophys Acta. 717:1982;398-404.
27. Williams DE, Masters BSS, Lech JJ, Buhler DR Sex differences in cytochrome P-450 isozyme composition and activity in kidney microsomes of mature rainbow trout. Biochem Pharmacol. 35:1986;2017-2023.