A high affinity gonadotropin-releasing hormone (GnRH) tracer, radioiodinated at position 6, facilitates analysis of mutant GnRH receptors

Endocrinology

Volumn 139, Issue 10, 1998, Pages 4115-4119

  Flanagan, Colleen A ^a,c   Fromme, Bernhard J ^a   Davidson, James S ^a   Millar, Robert P ^a,b  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

^b MRC Reproductive Biology Unit   (United Kingdom)

^c MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GONADORELIN; GONADOTROPIN RECEPTOR; TRACER;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; BINDING AFFINITY; CELL CULTURE; DATA ANALYSIS; GENE MUTATION; GONADOTROPIN RELEASE; HORMONE DETERMINATION; ISOTOPE LABELING; LIGAND BINDING; MOLECULAR CLONING; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; RADIOIODINATION; RECEPTOR AFFINITY; SITE DIRECTED MUTAGENESIS;

EID: 0031764251     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/endo.139.10.6260     Document Type: Article

References (21)

1. Sealfon SC, Weinstein H, Millar RF 1997 Molecular mechanisms of ligand interaction with the gonadotropin-releasing hormone receptor. Endocr Rev 18:180-205
2. Zhou W, Rodic V, Kitanovic S, Flanagan CA, Chi L, Weinstein H, Maayani S, Millar RP, Sealfon SC 1995 A locus of the gonadotropin-releasing hormone receptor that differentiates agonist and antagonist binding sites. J Biol Chem 270:18853-18857
3. Davidson JS, McArdle CA, Davies P, Elario R, Flanagan CA, Millar RP 1996 Asn102 of the gonadotropin-releasing hormone receptor is a critical determinant of potency for agonists containing C-terminal glycinamide. J Biol Chem 271:15510-15514
4. Clayton RN, Shakespear RA, Duncan JA, Marshall JC, Munson PJ, Rodbard D 1979 Radioiodinated nondegradable gonadotropin-releasing hormone analogs: new probes for the investigation of pituitary gonadotropin-releasing hormone receptors. Endocrinology 105:1369-1376
5. 11-coupled neuropeptide receptors; the GnRH receptor. In: Sealfon SC (ed) Methods in Neurosciences, Receptor Molecular Biology. Academic Press, San Diego, pp 145-162
6. Shinitzky M, Fridkin M 1976 Structural features of luliberin (luteinising hormone-releasing factor) inferred from fluorescence measurements. Biochim Biophys Acta 434:137-143
7. Sandow J, Konig W, Geiger R, Uhmann R, von Rechenberg W 1978 Structure-activity relationships in the LHRH molecule. In: Crighton DB, Haynes NB, Foxcroft GR, Lamming GE (eds) Control of Ovulation. Butterworths, London, pp 49-70
8. Perrin MH, Haas Y, Rivier JE, Vale WW 1983 Gonadotropin-releasing hormone binding to rat anterior pituitary membrane homogenates. Comparison of antagonists and agonists using radiolabeled antagonist and agonist. Mol Pharmacol 23:44-51
9. Kenakin T 1993 Pharmacologie Analysis of Drug-Receptor Interaction. Raven Press, New York
10. Karten MJ, Rivier JE 1986 Gonadotropin-releasing hormone analog design. Structure-function studies toward the development of agonists and antagonists: rationale and perspective. Endocr Rev 7:44-66
11. Clayton RN, Catt KJ 1980 Receptor-binding affinity of gonadotropin-releasing hormone analogs: analysis by radioligand-receptor assay. Endocrinology 106:1154-1159
12. Millar RP, Flanagan CA, Milton RC, King JA 1989 Chimeric analogues of vertebrate gonadotropin-releasing hormones comprising substitutions of the variant amino acids in positions 5, 7, and 8. Characterization of requirements for receptor binding and gonadotropin release in mammalian and avian pituitary gonadotropes. J Biol Chem 264:21007-21013
13. Hulme EC, Birdsall NJM 1992 Strategy and tactics in receptor-binding studies. In: Hulme EC (ed) Receptor-Ligand Interactions, A Practical Approach. Oxford University Press, Oxford, pp 63-174
14. Windle JJ, Weiner RI, Mellon PL 1990 Cell lines of the pituitary gonadotrope lineage derived by targeted oncogenesis in transgenic mice. Mol Endocrinol 4:597-603
15. Chi L, Zhou W, Prikhozhan A, Flanagan C, Davidson JS, Golembo M, Illing N, Millar RP, Sealfon SC 1993 Cloning and characterization of the human GnRH receptor. Mol Cell Endocrinol 91:R1-R6
16. Davidson JS, Flanagan CA, Davies PD, Hapgood J, Myburgh D, Elario R, Millar RP, Forrest-Owen W, McArdle CA 1996 Incorporation of an additional glycosylation site enhances expression of functional human gonadotropin-releasing hormone receptor. Endocrine 4:207-212
17. Keown WA, Campbell CR, Kucherlapati RS 1990 Methods for introducing DNA into mammalian cells. Methods Enzymol 185:527-537
18. Munson PJ, Rodbard D 1980 Ligand: a versatile computerized approach for characterization of ligand-binding systems. Anal Biochem 107:220-239
19. Coy DH, Coy EJ, Schally AV 1975 Structure-activity relationship of LH, FSH releasing hormone. In: Marks N, Rodnight R (eds) Research Methods in Neurochemistry. Plenum Press, New York, pp 393-406
20. Flanagan CA, Becker II, Davidson JS, Wakefield IK, Zhou W, Sealfon SC, Millar RP 1994 Glutamate 301 of the mouse gonadotropin-releasing hormone receptor confers specificity for arginine 8 of mammalian gonadotropin-releasing hormone. J Biol Chem 269:22636-22641
21. Nestor Jr JJ, Ho TL, Tahilramani R, McRea GI, Vickery BH 1984 Long acting LHRH agonists and antagonists. In: Labrie F, Belanger A, Dupont A (eds) LHRH and Its Analogues: Basic and Clinical Aspects. Elsevier, Amsterdam, pp 24-35