Electrophoretic characterization of chitinases as a tool for the identification of Trichoderma harzianum strains

Mycological Research

Volumn 102, Issue 3, 1998, Pages 373-377

  Schickler, Hedva ^a   Danin Gehali, Bat Chen ^b   Haran, Shoshan ^b   Chet, Ilan ^b  

^a Kennedy Leigh Ctr for Hort Res

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL PEST CONTROL; CHITINASE; ELECTROPHORESIS; ENDOCHITINASE; ENZYME ACTIVITY; FUNGUS IDENTIFICATION; ISOELECTRIC POINT; N ACETYL BETA GLUCOSAMINIDASE;

BIOCONTROL AGENT; FUNGUS; IDENTIFICATION METHOD; PROTEIN;

TRICHODERMA HARZIANUM;

EID: 0031763624     PISSN: 09537562     EISSN: None     Source Type: Journal    
DOI: 10.1017/S0953756297004929     Document Type: Article

References (20)

1. Bradford, M. M. (1976). A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye-binding. Analytical Biochemistry 72, 248-254.
2. Burdon, J. J. & Marshall, D. R. (1983). The use of isoenzymes in plant disease research. In Isozymes in Plant Genetics and Breeding (ed. S. D. Tanksley & T. J. Orton), 1A, pp. 401-412. Elsevier: Amsterdam.
3. Chet, I. (1987). Trichoderma: application, mode of action, and potential as a biocontrol agent of soilborne plant pathogenic fungi. In Innovative Approaches to Plant Disease Control (ed. I. Chet), pp. 137-160. John Wiley & Sons: New York.
4. Chet, I., Barak, Z. & Oppenheim, A. (1993). Genetic engineering of microorganisms for improved biocontrol activity. In Biological Perspectives in Plant Pathogen Control (ed. I. Chet), pp. 211-235. John Wiley & Sons: New York.
5. De La Cruz, J., Hydalgo-Gallego, A., Lora, J. M., Benitez, T., Pintor-Toro, J. A. & Llobell, A. (1992). Isolation and characterization of three chitinases from Trichoderma harzianum. European Journal of Biochemistry 206, 859-867.
6. Elad, Y., Chet, I. & Henis, Y. (1982). Degradation of plant pathogenic fungi by Trichoderma harzianum. Canadian Journal of Microbiology 28, 719-725.
7. Haran, S., Schickler, H., Oppenheim, A. & Chet, I. (1995). New components of the chitinolytic system of Trichoderma harzianum. Mycological Research 99, 441-446.
8. Haran, S., Schickler, H., Oppenheim, A. & Chet, I. (1996). Differential expression of Trichoderma harzianum chitinases during mycoparasitism. Phytopathology 86, 980-985.
9. Harman, G. E., Hayes, C. K., Lorito, M., Broadway, R. M., Di Pietro, A., Peterbauer, C. & Tronsmo, A. (1993). Chitinolytic enzymes of Trichoderma harzianum: purification of chitobiosidase and endochitinase. Phytopathology 83, 313-318.
10. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
11. Lorito, M., Hayes, C. K., Di Pietro, A., Woo, S. L. & Harman, G. E. (1994). Purification, characterization and synergistic activity of a glucan, 1,3-β-glucosidase and an N-acetyl-β-glucosaminidase from Trichoderma harzianum. Phytopathology 84, 398-405.
12. McGrew, B. R. & Green, M. (1990). Enhanced removal of detergent and recovery of enzymatic activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis: use of casein in gel wash buffer. Analytical Biochemistry 189, 68-74.
13. Okon, Y., Chet, I. & Henis, Y. (1973). Effects of lactose, ethanol and cycloheximide on the translation pattern of radioactive compounds and on sclerotium formation of Sclerotium rolfsii. Journal of General Microbiology 74, 251-258.
14. Papavizas, G. C. (1985). Trichoderma and Gliocladium: biology, ecology and the potential for biocontrol. Annual Review of Phytopathology 23, 23-54.
15. Rifai, M. A. (1969). A revision of the genus Trichoderma. Mycological Papers 115, 1-56.
16. Roberts, W. K. & Selitrennikoff, C. P. (1988). Plant and bacterial chitinases differ in antifungal activity. Journal of General Microbiology 134, 169-176.
17. Samuels, G. J. (1996). Trichoderma: a review of biology and systematics of the genus. Mycological Research 100, 923-935.
18. Sivan, A. & Chet, I. (1989). Degradation of fungal cell walls by lytic enzymes of Trichoderma harzianum. Journal of General Microbiology 135, 675-682.
19. Sivan, A. & Chet, I. (1992). Microbial control of plant diseases. In Environmental Microbiology (ed. R. Mitchell), pp. 335-354. Wiley-Liss: New York.
20. Zamir, D. & Chet, I. (1985). Application of enzyme electrophoresis for the identification of isolates in Trichoderma harzianum. Canadian Journal of Microbiology 31, 578-580.