Aspergillus in the Papanicolaou stain: Morphology, fluorescence and diagnostic feasibility

Cytopathology

Volumn 9, Issue 6, 1998, Pages 381-388

  Hettlich, C ^a   Kupper Th ^a   Wehle, K ^a   Pfitzer, P ^a  

^a NONE

Author keywords

Aspergillosis; Aspergillus; Cytology; Fluorescence; Fungal infection; Morphology; Papanicolaou stain

Indexed keywords

ARTICLE; ASPERGILLOSIS; ASPERGILLUS FUMIGATUS; ASPERGILLUS NIGER; AUTOFLUORESCENCE; CELL INFILTRATION; CYTODIAGNOSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; MORPHOLOGY; NONHUMAN; PAPANICOLAOU TEST; PRIORITY JOURNAL; RESPIRATORY SYSTEM;

ASPERGILLOSIS; ASPERGILLUS; COLORING AGENTS; HISTOCYTOCHEMISTRY; HUMANS; LUNG DISEASES, FUNGAL; MICROBIOLOGICAL TECHNIQUES; RETROSPECTIVE STUDIES; STAINING AND LABELING;

EID: 0031763279     PISSN: 09565507     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2303.1998.00123.x     Document Type: Article

References (33)

1. Virchow R. Beiträge zur Lehre von den beim Menschen vorkommenden pflanzlichen Parasiten. Arch Pathol Anat u Physiol, Virchow's 1856; 9: 557-93.
2. Denning DW, Follansbee SE, Scolaro M et al. Pulmonary Aspergillosis in the Acquired Immunodeficiency Syndrome. N Engl J Med 1991; 324: 654-62.
3. Gold J. Opportunistic fungal infections in patients with neoplastic disease. Am J Med 1984; 76: 458-63.
4. Malo JL, Hawkins R, Pepys J. Studies in chronic allergic bronchopulmonary aspergillosis (1-4). Thorax 1977; 32: 254-78.
5. Staib F. Grundsätzliches zur Epidemiologie, Diagnostik und Therapie aerogener invasiver Pilzinfektionen, unter besonderer Berücksichtigung der invasiven Aspergillose. In: Staffi F, Huhn D, eds. Pilzinfektionen bei abwehrgeschwächten Patienten. Berlin: Springer-Verlag, 1991: 1-16.
6. Young RC, Bennett JE, Vogel CL, Carbone PP, DeVita VT. Aspergillosis, the spectrum of the disease in 98 patients. Medicine 1970; 49: 147-73.
7. Yu VL, Muder RR, Poosattar A. Significance of isolation of Aspergillus from the respiratory tract in diagnosis of invasive pulmonary aspergillosis: results from a three-year prospective study. Am J Med 1986; 81: 249-54.
8. Smith FB, Beneck D. Localized Aspergillus infestation in primary lung carcinoma: clinical and pathological contrasts with post-tuberculous intracavitary aspergilloma. Chest 1991; 100: 554-6.
9. Wiest PM, Flanigan T, Salata RA et al. Serious infectious complications of corticosteroid therapy for COPD. Chest 1989; 95: 1180-4.
10. Fraser RS. Pulmonary Aspergillosis: pathologic and pathogenetic features. Pathol Annu 1993; 28: 231-77.
11. Koneman EW, Allen SD, Janda WM, Schreckenberger PC, Winn WC Jr. Color Atlas and Textbook of Diagnostic Microbiology, 4th edn. Philadelphia: J. B. Lippincott Co., 1992: 791-879.
12. Wehle K, Küpper Th, Marzahn S, Pfitzer P. Identification of phagocytosed Pneumocystis carinii in human alveolar macrophages. Cytopathol 1993; 4: 225-9.
13. Greenberger PA, Patterson R. Allergic bronchopulmonary aspergillosis. Model of bronchopulmonary disease with denned serologic, radiologic, pathologic and clinical findings from asthma to fatal destructive lung disease. Chest 1987; 91: 165S-171S.
14. Graham AR. Fungal autofluorescence with ultraviolet illumination. Am J Clin Pathol 1983; 79: 231-4.
15. Mann JL. Autofluorescence of fungi: an aid to detection in tissue sections. Am J Clin Pathol 1983; 79: 587-90.
16. Küpper Th, Wehle K, Plitzer P. Detection of Charcot-Leyden crystals in Papanicolaoustained cytological routine material of the respiratory system by fluorescence microscopy. Cytopathol 1994; 5: 262-9.
17. Ghali VS, Garcia RL, Skolom J. Fluorescence of Pneumocystis carinii in Papanicolaou smears. Hum Pathol 1984; 15: 907-9.
18. Hallier S. Untersuchungen zum Wachweis von Legionella sp. in Papanicolaou-gefärbten Präparaten des Respirationstraktes mittels direkter Fluoreszenzmikroskopie. Dissertation. Düsseldorf: Heinrich-Heine-University, 1996: 90.
19. Küpper Th, Wehle K, Marzahn S, Pfitzer P. The cytologic diagnosis of Mycobacterium kansasi tuberculosis by fluorescence microscopy of Papanicolaou stained specimens. Cytopathol 1995; 6: 331-8.
20. Küpper Th, Steffen U, Wehle K, Richartz G, Pfitzer P. Morphological study of bacteria of the respiratory system using fluorescence microscopy of Papanicolaou stained smears with special regard to Mycobacteria sp. Cytopathol 1995; 6: 338-402.
21. Pfitzer P, Wehle K, Blanke M, Bürrig KF. Fluorescence microscopy of Papanicolaoustained bronchoalveolar lavage specimens in the diagnosis of Pneumocystis carinii. Acta Cytol 1989; 33: 557-9.
22. Wehle K. Makrophageneinschlüsse in der Fluoreszenzmikroskopie Papanicolaougefärbter bronchoalveolärer Lavagen. GBK Fortbildung Aktuell 1994; 64: 26-29.
23. Wehle K, Blanke M, König G, Pfitzer P. The cytological diagnosis of Pneumocystis carinii by fluorescence microscopy of Papanicolaou stained bronchoalveolar lavage specimens. Cytopathol 1991; 2: 113-20.
24. Wehle K, Küpper Th, Pfitzer P. Fluorescence microscopy for Pneumocystis carinii. Recent Results Cancer Res 1993; 133: 117-21.
25. Raper KB, Fennel DI. The Genus Aspergillus. Baltimore: The Williams & Wilkins Co., 1965: 13-34, 238-68, 293-344.
26. Markham P, Robson GD, Bainbridge BW, Trinci APJ. Choline: its role in the growth of filamentous fungi and the regulation of mycelial morphology. FEMS Microbiol Rev 1993; 104: 287-300.
27. Robson GD, Wiebe MG, Trinci APJ. Exogenous cAMP and cGMP modulate branching in Fusarium graminaearum. J Gen Microbiol 1991; 137: 963-9.
28. Schaffner A, Douglas H, Braude A. Selective protection against Conidia by mononuclear and against mycelia by polymorphonuclear phagocytes in resistance to Aspergillus. J Clin Invest 1982; 69: 617-31.
29. Waldorf AR, Levitz SM, Diamond RD. In vivo bronchoalveolar macrophage defense against Rhizopus oryzae and Aspergillus fumigatus. J Infect Dis 1984; 150: 752-60.
30. Diamond RD, Krzesicki R, Epstein B, Jao W. Damage to hyphal forms of fungi by human leukocytes in vitro. Am J Pathol 1978; 91: 313-28.
31. Beaumont F. Clinical manifestations of Pulmonary Aspergillus infections. Mycoses 1988; 31 (Suppl. 2): 15-20.
32. Kaufmann HF, Beaumont F. Serological diagnosis of Aspergillus infections. Mycoces 1988; 31 (Suppl. 2): 21-26.
33. Levy H, Horak DA, Tegtmeier BR, Yokota SB, Fonnan SJ. The value of bronchoalveolar lavage and bronchial washings in the diagnosis of invasive pulmonary aspergillosis. Resp Med 1992; 86: 243-8.