Flavonoids stimulate Cl conductance of human airway epithelium in vitro and in vivo

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 275, Issue 5 19-5, 1998, Pages

  Illek, Beate ^a   Fischer, Horst ^a  

^a CHILDREN S HOSPITAL OAKLAND   (United States)

Author keywords

Apigenin; Chloride conductance; Cystic fibrosis; Cystic fibrosis transmembrane conductance regulator; Genistein; Kaempferol; Quercetin

Indexed keywords

APIGENIN; CHLORIDE; FLAVONOID; FORSKOLIN; GENISTEIN; ISOPRENALINE; KAEMPFEROL; QUERCETIN; TRANSMEMBRANE CONDUCTANCE REGULATOR;

ARTICLE; BINDING SITE; CHEMICAL STRUCTURE; CHLORIDE CURRENT; CHLORIDE TRANSPORT; GENE MUTATION; HUMAN; HUMAN TISSUE; MEMBRANE CONDUCTANCE; MICHAELIS CONSTANT; PATCH CLAMP; PRIORITY JOURNAL; PROBABILITY; RESPIRATORY EPITHELIUM; STATISTICAL ANALYSIS;

EID: 0031763141     PISSN: 10400605     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajplung.1998.275.5.l902     Document Type: Article

References (19)

1. Akiyama, T., and H. Ogawara. Use and specificity of genistein as inhibitor of protein tyrosine kinases. Methods Enzymol. 201: 362-369, 1991.
2. Alton, E. W. F. W., A. D. Currie, R. Logan-Sinclair, J. O. Warner, M. E. Hodson, and D. M. Geddes. Nasal potential difference: a clinical test for cystic fibrosis. Eur. Respir. J. 3: 922-926, 1990.
3. Arispe, N., J. Ma, K. Jacobson, and H. Pollard. Direct activation of cystic fibrosis transmembrane conductance regulator channels by 8-cyclopentyl-1,3-dipropylxanthine (CPX) and 1,3-diallyl-8-cyclohexylxanthine (DAX). J. Biol. Chem. 273: 5727-5734, 1998.
4. 2+-independent activation of cystic fibrosis transmembrane conductance regulator channels by phenylimidazothiazole drugs. J. Biol. Chem. 271: 16171-16179, 1996.
5. Chiang, C., S. Chen, M. Chang, C. Lin, and H. Luk. Genistein directly induces cardiac CFTR chloride current by a tyrosine kinase-independent and protein kinase A-independent pathway in guinea pig ventricular myocytes. Biochem. Biophys. Res. Commun. 235: 74-78, 1997.
6. Cliff, W. H., and R. A. Frizzell. Separate Cl conductances activated by cAMP and Ca in Cl secreting epithelial cells. Proc. Natl. Acad. Sci. USA 87: 4956-4960, 1990.
7. DeAzevedo, W. F., Jr., H. J. Mueller-Dieckmann, U. Schulze-Gahmen, P. J. Worland, E. Sausville, and S. H. Kim. Structural basis for specificity and potency of a flavonoid inhibi-tor of human CDK2, a cell cycle kinase. Proc. Natl. Acad. Sci. USA 93: 2735-2740, 1996.
8. De Vries, J. H., P. L. Janssen, P. C. Hollman, W. A. van Staveren, and M. B. Katan. Consumption of quercetin and kaempferol in free-living subjects eating a variety of diets. Cancer Lett. 114: 141-144, 1997.
9. Fischer, H., and T. E. Machen. CFTR displays voltage dependence and two gating modes during stimulation. J. Gen. Physiol. 104: 541-566, 1994.
10. Fischer, H., and T. E. Machen. The tyrosine kinase p60c-src regulates the fast gate of the cystic fibrosis transmembrane conductance regulator chloride channel. Biophys. J. 71: 3073-3082, 1996.
11. Formica, J. V., and W. Regelson. Review of the biology of quercetin and related flavonoids. Food Chem. Toxicol. 33: 1061-1080, 1995.
12. - channels via a tyrosine kinase- and protein phosphatase-independent mechanism. Am. J. Physiol. 273 (Cell Physiol. 42): C747-C753, 1997.
13. Goldman, D. E. Potential, impedance and rectification in membranes. J. Gen. Physiol. 27: 37-60, 1943.
14. Haws, C., W. E. Finkbeiner, J. H. Widdicombe, and J. J. Wine. CFTR in Calu-3 human airway cells: channel properties and role in cAMP-activated Cl conductance. Am. J. Physiol. 266 (Lung Cell. Mol. Physiol. 10): L502-L512, 1994.
15. Hwang, T., F. Wang, I. Yang, and W. Reenstra. Genistein potentiates wild-type and ΔF508-CFTR channel activity. Am. J. Physiol. 273 (Cell Physiol. 42): C988-C998, 1997.
16. Illek, B., H. Fischer, and T. E. Machen. Alternate stimulation of apical CFTR by genistein in epithelia. Am. J. Physiol. 270 (Cell Physiol. 39): C265-C275, 1996.
17. Illek, B., H. Fischer, G. F. Santos, J. H. Widdicombe, T. E. Machen, and W. W. Reenstra. cAMP-independent activation of CFTR Cl channels by the tyrosine kinase inhibitor genistein. Am. J. Physiol. 268 (Cell Physiol. 37): C886-C893, 1995.
18. - conductance through CFTR in human airway epithelia. Am. J. Physiol. 272 (Lung Cell. Mol. Physiol. 16): L752-L761, 1997.
19. Janssen, K., R. P. Mensink, F. J. Cox, J. L. Harryvan, R. Hovenier, P. C. Hollman, and M. B. Katan. Effects of the flavonoids quercetin and apigenin on hemostasis in healthy volunteers: results from an in vitro and a dietary supplement study. Am. J. Clin. Nutr. 67: 255-262, 1998.