Stationary organotypic cultures of oxytocin and vasopressin magnocellular neurones from rat and mouse hypothalamus

Journal of Neuroendocrinology

Volumn 10, Issue 11, 1998, Pages 849-861

  House, S B ^a   Thomas, A ^a   Kusano, K ^a   Gainer, H ^a,b  

^a NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

c fos; Depolarization; Magnocellular neurones; Neurohypophysis; Neurophysin; Slice explants; Veratridine

Indexed keywords

OXYTOCIN; POTASSIUM; VASOPRESSIN;

ADAPTATION; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL SURVIVAL; CONTROLLED STUDY; CULTURE MEDIUM; DEPOLARIZATION; GENE EXPRESSION; HYPOTHALAMUS; IMMUNOCYTOCHEMISTRY; MAGNOCELLULAR NUCLEUS; MOUSE; NERVE CELL; NEWBORN; NONHUMAN; ONCOGENE C FOS; ORGAN CULTURE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SUPRAOPTIC NUCLEUS; THALAMUS MIDLINE NUCLEUS;

ANIMALS; CELL POLARITY; CELL SURVIVAL; FLUORESCENT ANTIBODY TECHNIQUE; HYPOTHALAMUS; IMMUNOHISTOCHEMISTRY; MICE; NEURONS; ORGAN CULTURE TECHNIQUES; OXYTOCIN; PROTO-ONCOGENE PROTEINS C-FOS; RATS; RATS, SPRAGUE-DAWLEY; VASOPRESSINS;

EID: 0031762543     PISSN: 09538194     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2826.1998.00272.x     Document Type: Article

References (64)

1. Armstrong WE. Morphological and electrophysiological classification of hypothalamic supraoptic neurons. Prog Neurobiol 1995; 47: 291-339.
2. Hatton GI. Function-related plasticity in Hypothalamus. Annu Rer Neurosci 1997; 20: 375-397.
3. Gainer H, Wray S. Cellular and Molecular Biology of Oxytocin and Vasopressin. In: Knobil, E. & Neill, J. D. eds. The Physiolgy of Reproduction. New York: Raven Press, 1994: 1099-1129.
4. Stoppini L, Buchs PA, Muller D. A simple method for organotypic cultures of nervous system. J Neurosci Meth 1991; 37: 173-182.
5. Belenky M. Wagner S, Yarom Y, Matzner H, Cohen S. Castel M. The suprachiasmatic nucleus in stationary organotypic culture. Neurosci 1996; 70: 127-143.
6. Mezey E. Kiss JZ. Coexpression of vasopressin and oxytocin in hypothalamic supraoptic neurons of lactating rats. Endocrinology 1991; 129: 1814-1820.
7. Gamse R, Vaccaro DE, Gamse G, D.Pace M, Fox TO. Leeman SE. Release of immunoreactive somatostatin from hypothalamic cells in culture: inhibition by γ-aminobutyric acid. Proc Natl Acad Sci USA 1980: 77: 5552-5556.
8. Denizeau F, Dube D, Antakly T, Lemay A. Parent A, Pelletier G, Labrie F. Attempts to demonstrate peptide localization and secretion in primary cell cultures of fetal rat hypothalamus, Neuroendocrinology 1981; 32: 96-102.
9. Lolait SJ, Lim AT, Dahl D, Kalid BAK, Toh BH, Funder JW. Neonatal rat hypothalamus cell culture: neuron subpopulations secrete immunoreactive β-endorphin but not immunoreactive ACTH. Neuroendocrinology 1983; 37: 111-116.
10. Scanlon MF, Bobbins RJ, Bolaffi JL, Jackson IMD, Reichlin S. Characterization of somatostatin and TRH release by rat hypthalamic and cerebral cortical neurons maintained on a capillary membrane perfusion system. Neuroendocrinology 1983; 37: 269-274.
11. Clarke MJO, Lowry P, Gillies G. Assessment of corticotropin-releasing factor, vasopressin and somatostatin secretion by fetal hypothalamic neurons in culture, Neuroendocrinology 1987; 46: 147-154.
12. Bamberger AM, Pu LP, Cool DR, Loh YP. The neuro-2a neuroblastoma cell line expresses [Met]-enkephalin and vasopressin mRNA and peptide. Mol. Cell Endocrinol 1995; 13: 155-163.
13. Kai-Kai MA, Anderton BH, Keen P. A quantitative analysis of the interrelationships between subpopulations of rat sensory neurons containing arginine vasopressin or oxytocin and those containing substance P. fluoride- resistant acid phosphatase or neurofilament protein. Neuroscience 1986; 18: 475-486.
14. Jessell TM. Dodd J. Neurotransmitters and differentiation antigens in subsets of sensory neurons projecting to the spinal dorsal horn. In: Martin JB, Barchas JD eds. Neuropeptides in Neurologic and Psychiatric Disease. New York: Raven Press, 1986: 111-133.
15. Kai-Kai MA, Che YM. Distribution of arginine-vasopressin in the trigeminal, dorsal root ganglia and spinal cord of the rat: depletion by capsacin. Comp Biochem Physiol 1995; 110A: 71-78.
16. Goldstein ME, House SB, Gainer H. NF-L and peripherin immunoreactivities define distinct classes of rat sensory ganglion cells. J Neurosci Res 1991; 30: 92-104.
17. Hallbeck M, Hermanson O, Blonquist A. Preprovasopressin mRNA is not present in dorsal root ganglia of rat. Neuroscience Lett 1996; 209: 125-128.
18. Benda P. DeVitry R, Picart R, Tixier-Vidal A. Dissociated cell cultures from fetal mouse hypothalamus. Patterns of organization and ultrastructural features. Expl Brain Res 1975; 23: 29-47.
19. Jirikowski G, Reisert I. Pilgrim CH. Neuropeptides in dissociated cultures of hypothalamus and septum: quantitation of immunoreactive neurons. Neuroscience 1981; 6: 1953-1960.
20. Whatley SA, Hall C, Lim L. Hypothalamic Neurons in dissociated cell culture: the mechanism of increased survival times in the presence of non- neuronal cells. J Neurochem 1981; 36: 2052-2056.
21. Ventimiglia R, Geller HM. Cell-types and cell-substrate interactions in serum-free dissociated cultures of rat hypothalamus. Brain Res 1987; 436: 339-351.
22. Diaz H, Lorenzo A, Carrer HF, Caceres A. Time lapse study of neurite growth in hypothalamic dissociated neurons in culture: sex difference and estrogen effects. J Neurosci Ret 1992; 33: 266-281.
23. Wahle P, Muller TH, Swandulla D. Characterization of neurochemical phenotypes in cultured hypothalamic neurons with immunohistochemistry and in situ hybridization. Brain Res 1993; 611: 37-45.
24. DiScala Guenot D, Strosser MT, Sarlieve LL, Legros JJ, Richard P. Development of neurophysin-containing neurons in primary cultures of rat hypothalami is related to the age of the embryo: morphological study and comparison of in vivo and in vitro neurophysins, oxytocin, and vasopressin content. J Neurosci Res 1990; 25: 94-102.
25. Oeding P, Schilling K, Schmale H. Vasopressin expression in cultured neurons is stimulated by cyclic AMP. J Neuroendocrinol 1990; 2: 859-865.
26. Sladek CD, Gallagher MJ. The stimulation of vasopressin gene expression in cultured hypothalamic neurons by cyclic adenosine 3′ 5′-monophosphate is reversible. Endocrinology 1993; 133: 1322-1330.
27. Mathiasen JR, Larson ER, Ariano MA, Sladek CD. Neurophysin expression is stimulated by dopamine Dl agonist in dispersed hypothalamic cultures. Am J Physiol 1996; 270: R404-R412.
28. Lowe IP, Jaffe R, Yunis EJ, Mckelvy JF. Primary dispersed cell cultures of female rat hypothalamus in the early postnatal period. Peptides 1980; 1 (Suppl. 1): 211-219.
29. Bennett BA, Sundberg DK, Morris M. Evaluation of catecholaminergic activity in hypothalamic cultures. Meth Neurosciences 1990; 2: 330-341.
30. Yamashita N, Nishiyama N, Abe K, Saito H, Fukuda J. Primary culture of postnatal rat hypothalamic neurons in astrocyte-conditioned medium. Brain Rex 1992; 594: 215-220.
31. Bilinski M, Sanchez A, Nicolini VG, Villar MJ. Tramezzani JH. Dispersion and culture of magnocellular neurons from the supraoptic nucleus of the adult rat. J Neuroscience Meth 1996; 64: 13-18.
32. Feldman SC, Brown LL, Bornstein MB. Catecholamine-containing neurons in cultures of fetal rat hypothalamus: distribution, morphology, and maturation. Cell Molec Neurobiol 1981; 1: 279-288.
33. Masurovsky EB. Benity HH, Murray MR. Synaptic development in long term organized cultures of murine hypothalamus. J Comp Neurol 1971; 143: 263-277.
34. Toran-Allerand CD. Sex steroids and the development of the newborn mouse hypothalamus and preoptic area in vitro: implications for sexual differentiation. Brain Rex 1976; 106: 407-412.
35. Gähwiler BH. Organotypic monolayer cultures of nervous tissue. J Neurosci Meth 1981; 4: 329-342.
36. Gähwiler BH. Organotypic cultures of neural tissue. Trends Neurosci 1988; 11: 484-489.
37. Gähwiler BH, Capogna M, Debanne D, McKinney RA. Thompson SM. Organotypic slice cultures: a technique has come of age. Trends Neurosci 1997: 20: 471-477.
38. Wray S, Gähwiler BH, Gainer H. Slice cultures of LHRH neurons in the presence and absence of brainstem and pituitary. Peptides 1988; 9: 1151-1175.
39. Wray S, Castel M, Gainer H. Characterization of the suprachiasmatic nucleus in organotypic slice explant cultures. Microsc Res Tech 1993; 25: 46-80.
40. Gainer H, Kusano K, Wray S. Hypothalamic slice-explant cultures as models for the long-term study of gene expression and cellular activity. Regulatory Peptides 1993; 45: 25-29.
41. Bertini LT, Kursner C, Gaillard RC, Corder R, Kiss JZ. A tissue culture model of the hypophysiotrophic CRF producing neuronal system. Neuroendorinology 1993; 57: 716-728.
42. Gähwiler BH, Sandoz P, Dreifuss JJ. Neurons with synchronous bursting discharges in organotypic cultures of the hypothalamic supraoptic nucleus area. Brain Res 1978; 151: 245-253.
43. Gähwiler BH, Dreifuss JJ. Phasically firing neurons in long-term cultures of the rat hypothalamic supraoptic area: pacemaker and follower cells. Brain Res 1979; 177: 95-103.
44. Sofroniew MV, Dreifuss JJ. Gähwiler BH. Slice cultures of rat hypothalamus examined by immunohistochemical staining for neurohypophyseal peptides and GFAP. Brain Res Bull 1988; 20: 669-674.
45. Wray S. Organotypic Slice Explant Roller-Tube Cultures. In: Boulton A, Baker G, and Walz W eds. Practical Cell Culture Techniques, Vol. 23. New York: Humane Press Inc., 1992: 201-239.
46. Wray S, Kusano K, Gainer H. Maintenance of LHRH and Oxytocin neurons in slice-explants cultured in serum-free media: effects of tetrodotoxin on gene expression, Neuroendocrinology 1991; 54: 327-339.
47. Rhodes CH, Morrell JI, Pfaff DW. Immunohistochemical analysis of magnocellular elements in rat hypothalamus: distribution and numbers of cells containing neurophysin, oxytocin, and vasopressin. J Comp Neural 1981; 198: 45-64.
48. Swaab DF, Nijveldt F, Pool CW. Distribution of oxytocin and vasopression in the rat supraoptic and paraventricular nucleus. J Endocrinol 1975; 67: 461-462.
49. Shen E. Dun SL. Ren C, Bennett-Clarke C, Dun NJ. Hypotension preferentially induces c-fos imunoreactivity in supraoptic vasopressin neurons. Brain Res 1992; 593: 136-139.
50. Harding AJ, Ng JLF. Halliday GM, Oliver J. Comparison of the number of vasopressin-producing hypothalamic neurons in rats and humans. J Neuroendocrinol 1995; 7: 629-636; 826.
51. Carter DA. Murphy D. Regulation of c-fos and c-jun expression in the rat supraoptic nucleus. Cell Mol Neurobiol 1990; 10: 435-45.
52. Fenelon VS, Poulain DA, Theodosis DT. Oxytocin neuron activation and fos expression: a quantitative immunocytochemical analysis of the effect of lactation, parturition, osmotic and cardiovascular stimulation. Neuroscience 1993; 53: 77-89.
53. Hoffman GE, Smith MS, Verbalis JG. c-Fos and related immediate-early gene products as markers of activity in neuroendocrine systems, Front Neuroendocrinol 1993; 14: 173-213.
54. Luckman S, Dyball RFJ, Leng G. Induction of c-fos expression in hypothalamic magnocellular neurons requires synaptic activation and not simply increased spike activity. J Neuroscience 1994; 14: 4825-4830.
55. Chan RKW. Brown ER, Ericsson A, Kovac KJ, Sawchenko PE. A comparison of immediate-early genes, c-fos and NGFI-B, as markers for functional activation in Stress-related neuroendocrine circuitry. J Neuroscience 1993; 13: 5126-5138.
56. Ericksson M, Ceccatelli S, Urnas-Moberg K, Iadarola M, Hokfelt T. Lxpression of fos -related antigens, oxytocin, dynorphin, and galanin in The paraventricular and supraoptic nuclei of lactating rals. Neuroendocrinol 1996; 63: 356-367.
57. Wang K, Guldenaar SEF, McCabe JT. Fos and Jun expression in rat supraoptic nucleus after acute vs. repeated osmotic stimulation. Brain Res 1997; 746: 117-125.
58. Mason WT. Supraoptic neurones of rat hypothalamus are osmosensitive. Nature 1980; 287: 154-157.
59. Bourque CW. Ionic basis for the intrinsic activation of rat supraoptic neurons by hyperosmotic stimuli. J Physiol (Lond) 1989; 417: 263-277.
60. Bourque CW, Oliet SH. Osnioreceptors in the central nervous system. Ann RcV Physiol 1997; 59: 601-619.
61. Ludwig M. Johnstone LE, Neumann I, Landgraf R, Russell JA. Direct hypertonic stimulation of the rat supraoptic nucleus increases c-fos expression in glial cells rather than magnocellular neurons Cell Tiss Res 1997; 287: 79-90.
62. Ben-Barak Y, Russell JT, Whitnall MH, Ozato K, Gainer H. Neurophysin in the hypothalamo- neurohypophysial system. 1: Production and characterization of monoclonal antibodies. J Neurosci 1985; 5: 81-97.
63. Watson RE Jr. Wiegand SJ, Clough RW. Hoffman GE. Use of cryoprotectant to maintain long-term peptide immunoreactivity and tissue morphology. Peptides 1986; 7: 155-159.
64. Hsu SM, Soban E. Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry. J Histochem and Cytochem 1982; 30: 1079-1082.