Isolation, characterization and ex vivo expansion of CD34+ cells from umbilical cord blood

Cancer Research Therapy and Control

Volumn 5, Issue 3, 1998, Pages 151-159

  Pasino, Mirella ^a,b   Biglino, Paola ^a   Corcione, Anna ^a   Scuderi, Francesca ^a   Franchini, Edoardo ^a   Tasso, Paola ^a   De Biasio, Pierangela ^a   Marotta, Fernando ^a   Valetto, Angelo ^a   Pistoia, Vito ^a   Mori, Pier Giorgio ^a  

^a Department of Pediatric Hematology and Oncology ^*

^b G GASLINI INSTITUTE   (Italy)

Author keywords

Cytokines; Ex vivo expansion; Hemopoietic progenitors; Immunophenotype; Umbilical cord blood

Indexed keywords

CD33 ANTIGEN; CD34 ANTIGEN; CYTOKINE; ERYTHROPOIETIN; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; HLA DR ANTIGEN; INTERLEUKIN 3; INTERLEUKIN 6; STEM CELL FACTOR;

ALLOGRAFT; ARTICLE; BONE MARROW; BURST FORMING UNIT E; CELL ISOLATION; CELL SELECTION; COLONY FORMING UNIT E; COLONY FORMING UNIT GM; CONTROLLED STUDY; FLOW CYTOMETRY; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; HUMAN CELL; LYMPHOCYTE; PRIORITY JOURNAL; UMBILICAL CORD BLOOD;

EID: 0031761276     PISSN: 10640525     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Abboud, M., Xu, F., La Via, M. and Laver, J. (1992). Study of early hematopoietic precursors in human cord blood. Exp. Hematol., 20, 1043-1047
2. Barcena, A., Muench, M.O., Galy, A.H.M., Cupp, J., Roncarolo, M.G. and Phillips, J.H. (1993). Phenotypic and functional analysis of T-cell precursors in the human fetal liver and thymus: expression in the early stages of T- and myeloid-cell development. Blood, 82, 3401-3414.
3. Bertolini, F., Soligo, D., Lazzari, L., Corsini, C., Servida, F. and Sirchia, G. (1995). The effect of interleukin-12 in ex vivo expansion of human hematopoietic progenitors. Br. J. Haematol., 90, 935-938.
4. Broxmeyer, H.E., Douglas, G.W., Hangoc, G., Cooper, S., Bard, J., English, D., Arny, M., Thomas, L. and Boyse, E.A. (1989). Human umbilical cord blood: as a potential source of transplantable hematopoietic stem/progenitor cells. Proc. Natl. Acad. Sci. USA, 86, 3828-3832.
5. Broxmeyer, H.E., Hangoc, G., Cooper, S., Ribeiro, R.C., Graves, V., Yoder, M., Wagner, J., Vadhan-Ray, S., Benninger, L., Rubinstein, P. and Bronnen, R.E. (1992). Growth characteristics and expansion of human umbilical cord blood and estimation of its potential for transplantation in adults. Proc. Natl. Acad. Sci. USA, 89, 4109-4113.
6. Carow, C.E., Hangoc, G. and Broxmeyer, H.E. (1993). Human multipotential progenitors cells (CFU-GEMM) have extensive replating capacity for secondary CFU-GEMM: an effect enhanced by cord blood plasma. Blood 81, 942-949.
7. + cells from bone marrow, umbilical cord and peripheral blood (primed for apheresis). using five separation systems. Stem Cells, 13, 524-532.
8. Di Giusto, D.L., Lee, R. and Moon, J. (1996). Hematopoietic potential of cryopreserved and ex vivo manipulated umbilical cord blood progenitor cells evaluated in vitro and in vivo. Blood, 87, 1261-71.
9. + human umbilical cord blood cells. Leuk. Lymphoma, 11, 263-273.
10. + cells after stimulation with recombinant human granulocyte-monocyte colony stimulating factor (CSF), granulocyte-CSF, monocyte-CSF, and interleukin 3. Blood, 78, 3192-3199.
11. Emerson, S.G. (1996). Ex vivo expansion of hematopoietic precursors, progenitors and stem cells: the next generation of cellular therapeutics. Blood,87, 3082-3088.
12. Fritsch, G., Buchinger, P., Printz, D., Fink, F.M., Mann, G., Peters, C., Wagner, T., Adler, A. and Gadner, H. (1993). Rapid discrimination of early CD34 myeloid progenitors using CD45-RA analysis. Blood, 81, 2301-2309.
13. Hamley, M.E., Nolta, J.A., Parkman, R. and Kohn, D.B. (1994). Umbilical cord blood cell transduction by retroviral vectors: preclinical studies to optimize gene transfer. Blood Cells20, 539-546.
14. + hematopoietic stem cells from human peripheral blood by high gradient magnetic cell sorting. Cytometry14, 384-392.
15. + hematopoietic stem cells from cord blood. Blood Cells,20, 371-375.
16. - hematopoietic progenitors cells by sequential immunoadsorption (Ceprate SC) and fluorescence
17. activated cell sorting: implications for gene transduction and for transplantation. Bone Marrow Transplant13, 649-654.
18. Laver, J.H., Abboud, M.R., Kawashima, I., Leary, A.G., Ashman, C.K. and Ogawa, M. (1995). Characterization of c-kit expression by primitive hematopoietic progenitors in umbilical cord blood. Exp. Hematol., 23, 1515-1519.
19. ++ human umbilical cord blood hematopoietic progenitors with high proliferative and replating potential. Blood,81, 41-48.
20. Mayani, H., Landsorp, P.M. (1994). Thy-1 expression is linked to functional properties of primitive hematopoietic progenitor cells from human umbilical cord blood. Blood83,2410-2417.
21. Mayani, H., Landsorp, P.M. (1995). Proliferation of individual hematopoietic progenitors purified from umbilical cord blood. Exp. Hematol.,23, 1453-1462.
22. Migliaccio, A.R., Migliaccio, G. and Adamson, J.W. (1994). Expansion of human neonatal progenitor cells in vitro under serum deprived conditions. Blood Cells20, 424-429.
23. +cord blood cells in the presence of recombinant human stem cell factor. Blood, 79, 2620-2627.
24. Nakahata, T. and Ogawa, M. (1982). Hematopoietic colonyforming cells in umbilical cord blood with extensive capability to generate mono-and multipotential hemopoietic progenitors. J. Clin. Invest.,70, 1324-1328.
25. + cells purified after cryopreservation. Exp. Hematol., 23, 1589-1594.
26. Ogawa, M. (1993). Differentiation and proliferation of hematopoietic stem cells. Blood,81, 2844-2853.
27. + hematopoietic cells. Exp. Hematol.,20, 24-33.
28. + hematopoietic progenitor cells. Blood, 86,4115-4123.
29. + cord blood hematopoietic progenitors in the presence and absence of growth factors. Blood Cells, 20, 517-524.
30. +cell population derived from human umbilical cord blood using flow cytometry and cDNA-polymerase chain reaction. Blood, 832103-2114.
31. + cells after stimulation with recombinant human granulocyte-monocyte colony-stimulating factor (CSF), granulocyteCSF, monocyte-CSF, and interleukin-3. Blood, 78, 3192-3199.
32. + cells an enhanced ex vivo expansion potential. Exp. Hematol.,22, 1264-1272.
33. Williams, D.A. (1993). Ex vivo expansion of stem and progenitor cells- Robbing Peter to pay Paul?. Blood, 81, 3469-3472.
34. + progenitors cell isolation from blood and marrow: a comparison of techniques for small-scale selection. Bone Marrow Transplant., 14, 265-271.