A new tool for studying the molecular architecture of the fungal cell wall: One-step purification of recombinant Trichoderma β-(1-6)-glucanase expressed in Pichia pastoris

Biochimica et Biophysica Acta - General Subjects

Volumn 1425, Issue 2, 1998, Pages 419-424

  Bom, I J ^a   Dielbandhoesing, S K ^a   Harvey, K N ^b   Oomes, S J C M ^a   Klis, F M ^c   Brul, S ^a  

^a UNILEVER RESEARCH VLAARDINGEN   (Netherlands)

^b UNIVERSITY OF ABERDEEN   (United Kingdom)

^c UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

(1 6) Glucanase; Enzyme purification; Fungal cell wall; Heterogeneous protein expression; Mannoprotein

Indexed keywords

GLUCAN GLUCOSIDASE; RECOMBINANT ENZYME;

ARTICLE; CELL WALL; CHROMATOGRAPHY; CONTROLLED STUDY; CULTURE MEDIUM; ENDOMYCETALES; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME STABILITY; FUNGUS CULTURE; ISOELECTRIC POINT; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRICHODERMA;

CELL MEMBRANE; ENZYME STABILITY; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; PICHIA; RECOMBINANT PROTEINS; TRICHODERMA;

HYPOCREA LIXII; PICHIA PASTORIS; TRICHODERMA;

EID: 0031756531     PISSN: 03044165     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0304-4165(98)00096-8     Document Type: Article

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