PRS5, the fifth member of the phosphoribosyl pyrophosphate synthetase gene family in Saccharomyces cerevisiae, is essential for cell viability in the absence of either PRS1 or PRS3

Journal of Bacteriology

Volumn 180, Issue 23, 1998, Pages 6404-6407

  Hernando, Yolanda ^a   Parr, Adrian ^a   Schweizer, Michael ^a  

^a INSTITUTE OF FOOD RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSEPHOSPHATE PYROPHOSPHOKINASE;

ARTICLE; CELL VIABILITY; ENZYME ACTIVITY; ENZYME SYNTHESIS; FUNGAL GENETICS; GENE DELETION; GROWTH REGULATION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN SYNTHESIS REGULATION; SACCHAROMYCES CEREVISIAE;

FUNGI; MYXOGASTRIA; SACCHAROMYCES CEREVISIAE;

EID: 0031755842     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.23.6404-6407.1998     Document Type: Article

References (16)

1. Boeke, J. D., F. Lacroute, and G. R. Fink. 1984. A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 197:345-346.
2. Bower, S. G., K. W. Harlow, R. L. Switzer, and B. Hove-Jensen. 1989. Characterization of the Escherichia coli prsA1-encoded mutant phosphoribosylpyrophosphate synthetase identifies a divalent cation-nucleotide binding site. J. Biol. Chem. 264:10287-10291.
3. Carter, A. T., A. Narbad, B. M. Pearson, K. F. Beck, M. Logghe, R. Contreras, and M. Schweizer. 1994. Phosphosribosylpyrophosphate synthetase: a new gene family in Saccharomyces cerevisiae. Yeast 10:1031-1044.
4. Carter, A. T., F. Beiche, B. Hove-Jensen, A. Narbad, P. J. Barker, L. M. Schweizer, and M. Schweizer. 1997. PRS1 is a key member of the gene family encoding phosphoribosylpyrophosphate synthetase in Saccharomyces cerevisiae. Mol. Gen. Genet. 254:148-156.
5. Gietz, R. D., and R. A. Woods. 1994. High efficiency transformation with lithium acetate, p. 121-131. In J. R. Johnson (ed.), Molecular genetics of yeast: a practical approach. IRL Press, Oxford, United Kingdom.
6. Güldener, U., S. Heck, T. Fiedler, J. Beinhauer, and J. H. Hegemann. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res. 24:2519-2524.
7. Hove-Jensen, B., K. W. Harlow, C. J. King, and R. L. Switzer. 1986. Phosphoribosylpyrophosphate synthetase of Escherichia coli. Properties of the purified enzyme and primary structure of the prs gene. J. Biol. Chem. 261: 6765-6771.
8. Kaiser, C., S. Michaelis, and A. Mitchell. 1994. Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
9. Khorana, H. G., J. F. Fernandes, and A. Kornberg. 1958. Pyrophosphorylation of ribose-5-phosphate in the enzymatic synthesis of 5-phosphorylribose 1-pyrophosphate. J. Biol. Chem. 230:941-948.
10. Mannhaupt, G., I. Vetter, C. Schwarzlose, S. Mitzel, and H. Feldmann. 1996. Analysis of a 26 kb region of the left arm of yeast chromosome XV. Yeast 12:67-76.
11. Ochi, K. 1987. Changes in nucleotide pools during sporulation in Streptomyces griseus in submerged culture. J. Gen. Microbiol. 133:2787-2795.
12. Pearson, B. M., Y. Hernando, and M. Schweizer. 1998. Construction of PCR-ligated long flanking homology cassettes for use in the functional analysis of six unknown open reading frames from the left and right arms of Saccharomyces cerevisiae chromosome XV. Yeast 14:391-399.
13. Sakakibara, Y. 1992. dnaR function of the prs gene of Escherichia coli in initiation of chromosome replication. J. Mol. Biol. 226:989-996.
14. Strauch, E., E. Takano, H. A. Baylis, and M. J. Bibb. 1991. The stringent response in Streptomyces coelicolor A3(2). Mol. Microbiol. 5:289-298.
15. Switzer, R. L, 1969. Regulation and mechanism of phosphoribosylpyrophosphate synthetase. J. Biol. Chem. 244:2854-2863.
16. Wach, A., A. Brachat, R. Pöhlmann, and P. Philippsen. 1994. New heterologous modules for classical or PCR-based gene disruption in Saccharomyces cerevisiae. Yeast 10:1793-1808.