Fluorescence-based SSCP analysis with automatic allele detection demonstrated for the factor V Leiden mutation

Clinical Chemistry

Volumn 44, Issue 9, 1998, Pages 1986-1987

  Geisel, Jürgen ^a   Walz, Tanja ^a   Bodis, Marion ^a   Quast, Sabine ^a   Herrmann, Wolfgang ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD CLOTTING FACTOR 5;

ARTICLE; BLOOD CLOTTING; DNA DETERMINATION; FLUORESCENCE; POINT MUTATION; SINGLE STRAND CONFORMATION POLYMORPHISM;

EID: 0031754386     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/44.9.1986     Document Type: Article

References (5)

1. Bertina RM, Koeleman BPC, Koster T, Rosendaal FR, Dirven RJ, de Ronde H, et al. Mutation in blood coagulation factor V associated with resistance to activated protein C. Nature 1994;369:64-7.
2. Rabès JP, Trossaert M, Conard J, Samama M, Giraudet P, Boileau C. Single point mutation at Arg506 of factor V associated with APC resistance and venous thromboembolism: improved detection by PCR-mediated site-directed mutagenesis. Thromb Haemost 1995;75:1379-80.
3. Lewandowski K, Rozek M, Zawilska H, Markiewicz WT. An alternative method for identifying the factor V gene Leiden mutation. Thromb Res 1997;85: 105-13.
4. 506→Gln) causing protein C resistance. Thromb Res 1996;83:87-96.
5. Corral J, Iniesta JA, González-Conejero R, Vicente V. Detection of factor V Leiden from a drop of blood by PCR-SSCP, Thromb Haemost 1996;76:735-7.