A two focal plane method for digital quantification of nuclear immunoreactivity in large brain areas using NIH-image software

Brain Research Protocols

Volumn 2, Issue 4, 1998, Pages 264-272

  Brown, Harold E ^a   Garcia, Meredith M ^a   Harlan, Richard E ^a  

^a TULANE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Caudate putamen; Digital image analysis; Image software; Immediate early gene protein; Immunocytochemistry

Indexed keywords

ARTICLE; CELL COUNT; CELL SIZE; COMPUTER PROGRAM; IMAGE DISPLAY; IMAGE QUALITY; IMAGING SYSTEM; IMMUNOCYTOCHEMISTRY; IMMUNOREACTIVITY; PRIORITY JOURNAL;

ANIMALS; CELL COUNT; CELL NUCLEUS; CELL SIZE; IMAGE PROCESSING, COMPUTER-ASSISTED; IMMUNOHISTOCHEMISTRY; NATIONAL INSTITUTES OF HEALTH (U.S.); NERVE TISSUE PROTEINS; PROTO-ONCOGENE PROTEINS C-FOS; PROTO-ONCOGENE PROTEINS C-JUN; RATS; SIGNAL PROCESSING, COMPUTER-ASSISTED; SOFTWARE; UNITED STATES;

EID: 0031748248     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(98)00003-8     Document Type: Article

References (20)

1. Allen G.V., Zhou J., Hopkins D.A. Monoamines in the parabrachial nucleus of the cardiomyopathic hamster. Brain Res. 680:1995;117-127.
2. Auger A.P., Blaustein J.D. Progesterone enhances an estradiol-induced increase in Fos immunoreactivity in localized regions of female rat forebrain. J. Neurosci. 15:1995;2272-2279.
3. Bot G., Chahl L.A. Induction of Fos-like immunoreactivity by opioids in guinea-pig brain. Brain Res. 731:1996;45-56.
4. H.E. Brown, M.M. Garcia, D. D'Souza, C.M. Lynch, R.E. Harlan, Chronic anabolic steroid treatment blunts morphine-mediated c-Fos induction: possible role of β-endorphin, Brain Res., submitted, 1997.
5. Francis R.C., Lee H.N., Fernald R.D. Ontogeny of gonadotropin releasing hormone-containing neurons in the teleost brain. Brain Res. Dev. Brain Res. 78:1994;151-160.
6. Garcia M.M., Brown H.E., Harlan R.E. Alterations in immediate-early gene proteins in the rat forebrain induced by acute morphine injection. Brain Res. 692:1995;23-40.
7. Gerhart D.Z., Djuricic B., Drewes L.R. Quantitative immunocytochemistry (image analysis) of glucose transporters in the normal and postischemic rodent hippocampus. J. Cereb. Blood Flow Metab. 11:1991;440-448.
8. Grandis J.R., Melhem M.F., Barnes E.L., Tweardy D.J. Quantitative immunohistochemical analysis of transforming growth factor-alpha and epidermal growth factor receptor in patients with squamous cell carcinoma of the head and neck. Cancer. 78:1996;1284-1292.
9. Huang X., Chen S., Tietz E.I. Immunocytochemical detection of regional protein changes in rat brain sections using computer-assisted image analysis. J. Histochem. Cytochem. 44:1996;981-987.
10. G.L. Humason, Animal Tissue Techniques, 3rd edn., Freeman, San Fransisco, 1972.
11. Johnson B.A., Woo C.C., Duong H., Nguyen V., Leon M. A learned odor evokes an enhanced Fos-like glomerular response in the olfactory bulb of young rats. Brain Res. 699:1995;192-200.
12. Johnsson A., Olsson C., Anderson H., Cavallin S.E. Evaluation of a method for quantitative immunohistochemical analysis of cisplatin-DNA adducts in tissues from nude mice. Cytometry. 17:1994;142-150.
13. Lambert P.D., Ely T.D., Gross R.E., Kilts C.D. Neurotensin induces fos and zif268 expression in limbic nuclei of the rat brain. Neuroscience. 75:1996;1141-1151.
14. Mize R.R. Quantitative image analysis for immunocytochemistry and in situ hybridization. J. Neurosci. Methods. 54:1994;219-237.
15. S. Roesch, P. Mailly, J.M. Deniau, Y. Maurin, Computer assisted three-dimensional reconstruction of brain regions from serial section digitized images, Application to the organization of striato-nigral relationships in the rat, J. Neurosci. Methods, (1996) 197-204.
16. J.C. Russ, The Image Processing Handbook, 2nd edn., CRC Press, Boca Raton, 1995.
17. Simpson J.N., McGinty J.F. Forskolin increases phosphorylated-CREB and fos immunoreactivity in rat striatum. Neuroreport. 5:1994;1213-1216.
18. Simpson J.N., Wang J.Q., McGinty J.F. Repeated amphetamine administration induces a prolonged augmentation of phosphorylated cyclase response element-binding protein and Fos-related antigen immunoreactivity in rat striatum. Neuroscience. 69:1995;441-457.
19. Sladek C.D., Olschowka J.A. Dehydration induces Fos, but not increased vasopressin mRNA in the supraoptic nucleus of aged rats. Brain Res. 652:1994;207-215.
20. Woo C.C., Oshita M.H., Leon M. A learned odor decreases the number of Fos-immunopositive granule cells in the olfactory bulb of young rats. Brain Res. 716:1996;149-156.