Rapid and ultrasensitive enzyme immunoassay (thin aqueous layer immune complex transfer enzyme immunoassay) for HIV-1 p24 antigen

Journal of Clinical Laboratory Analysis

Volumn 12, Issue 4, 1998, Pages 205-212

  Ishikawa, Setsuko ^a   Hashida, Seiichi ^a   Hashinaka, Kazuya ^a   Ishikawa, Eiji ^a  

^a UNIVERSITY OF MIYAZAKI   (Japan)

Author keywords

D galactosidase; Enzyme immunoassay; HIV 1; p24 antigen

Indexed keywords

ANTIGEN P24; BETA GALACTOSIDASE;

ANALYTIC METHOD; ANIMAL CELL; ANTIGEN ANTIBODY COMPLEX; ANTIGEN DETECTION; ARTICLE; COMPLEX FORMATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME IMMUNOASSAY; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; RECEPTOR AFFINITY; SEROCONVERSION;

ACQUIRED IMMUNODEFICIENCY SYNDROME; ADULT; AGED; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIGEN-ANTIBODY COMPLEX; FEMALE; HIV ANTIGENS; HIV CORE PROTEIN P24; HIV-1; HUMANS; IMMUNOENZYME TECHNIQUES; IMMUNOGLOBULIN FAB FRAGMENTS; MALE; MICE; MIDDLE AGED; POLYSTYRENES; RABBITS; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

ANIMALIA; BOVINAE; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; ORYCTOLAGUS CUNICULUS;

EID: 0031747347     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2825(1998)12:4<205::AID-JCLA3>3.0.CO;2-6     Document Type: Article

References (11)

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2. Hashida S, Hashinaka K, Hirota K, et al.: Detection of antibody IgG to HIV-1 in urine by ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant p24 as antigen for diagnosis of HIV-1 infection. J Clin Lab Anal 8:86-95, 1994.
3. Hashinaka K, Hashida S, Hirota K, et al.: Detection of antihuman immunodeficiency virus type 1 (HIV-1) immunoglobulin G in urine by an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) with recombinant reverse transcriptase as an antigen. J Clin Microbiol 32:819-822, 1994.
4. Hashida S, Hashinaka K, Saitoh A, et al.: Diagnosis of HIV-1 infection by detection of antibody IgG to HIV-1 in urine with ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) using recombinant proteins as antigens. J Clin Lab Anal 8:237-246, 1994.
5. Ishikawa S, Hashida S, Hashinaka K, et al.: Diagnosis of HIV-1 infection with whole saliva by detection of antibody IgG to HIV-1 with ultrasensitive enzyme immunoassay using recombinant reverse transcriptase as antigen. J Acquir Immune Defic Syndr Human Retrovirol 10:41-47, 1995.
6. Ishikawa S, Hashida S, Hashinaka K, et al.: Whole saliva dried on filter paper for diagnosis of HIV-1 infection by detection of antibody IgG to HIV-1 with ultrasensitive enzyme immunoassay using recombinant reverse transcriptase as antigen. J Clin Lab Anal 10:35-41, 1996.
7. Hashida S, Hashinaka K, Nishikata I, et al.: Immune complex transfer enzyme immunoassay that is more sensitive and specific than Western blotting for detection of antibody immunoglobulin G to human immunodeficiency virus type 1 in serum with recombinant pol and gag proteins as antigens. Clin Diag Lab Immunol 2.535-541, 1995.
8. Hashida S, Hashinaka K, Nishikata I, et al.: Shortening of the window period in diagnosis of HIV-1 infection by simultaneous detection of p24 antigen and antibody IgG to p17 and reverse transcriptase in serum with ultrasensitive enzyme immunoassay. J Virol Methods 62:43-53, 1996.
9. Hashida S, Hashinaka K, Nishikata I, et al.: Measurement of human immunodeficiency virus type 1 p24 in serum by an ultrasensitive enzyme immunoassay, the two-site immune complex transfer enzyme immunoassay. J Clin Microbiol 33:298-303, 1995.
10. Hashida S, Hashinaka K, Nishikata I, et al.: Ultrasensitive and more specific enzyme immunoassay (immune complex transfer enzyme immunoassay) for p24 antigen of HIV-1 in serum using affinity-purified rabbit anti-p24 Fab′ and monoclonal mouse anti-p24 Fab′. J Clin Lab Anal 10:302-307, 1996.
11. Ishikawa S, Hashida S, Hashinaka K, et al.: More sensitive immune complex transfer enzyme immunoassay for antibody IgG to p17 of HIV-1 with shorter incubation time for immunoreactions and larger volumes of serum samples. J Clin Lab Anal 11: 244-250, 1997.