A simple flat embedding method for the correlative light and electron microscopic immunocytochemistry

Microscopy

Volumn 47, Issue 5, 1998, Pages 535-537

  Sawada, Hajime ^a   Esaki, Michiyo ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Adar film; embedding; Epon; immunocytochemistry; immunoelectron microscopy; OHP film

Indexed keywords

ADHESIVES; ELECTRON MICROSCOPES;

ADAR FILM; EMBEDDING METHOD; EMBEDDINGS; EPON; IMMUNOCYTOCHEMISTRY; IMMUNOELECTRON MICROSCOPY; OHP FILM; SIMPLE METHOD; SIMPLE++; TISSUE SECTIONS;

EMBEDDINGS;

EID: 0031743883     PISSN: 20505698     EISSN: 20505701     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jmicro.a023625     Document Type: Article

References (9)

1. Aides L D and Boone T B (1984) A combined flat-embedding, HRP histochemical method for correlative light and electron microscopic study of single neurons. J Neumsa Res. 11: 27-34.
2. Reymond O L and Pickett-Heaps J D (1983) A routine flat embedding method for electron microscopy of microorganisms allowing selection and precisely orientated sectioning of single cells by light microscopy. J. Micmsc. 130: 79-84.
3. De Vries E, van der Weij JP. van Buysen AC and Cats A (1980) A Teflon culture and embedding device for the study of cells by light and electron microscopy. J Immunol. Meth. 36: 81-87.
4. De Felipe J and Fairen A (1993) A simple and reliable method for correlative light and electron microscopic studies. J. Histochem. Cytochcm. 41: 769-772.
5. Barnes K, Turner A J and Kenny A J (1988) Electronmicroscopic immunocytochemistry of pig brain shows that endopeptidase-24.11 is localized in neuronal membranes. Neurosci. Lett. 94: 64-69.
6. Sawada H and Esaki M (1994) Use of nanogold followed by silver enhancement and gold toning for postembedding immunolocalization in osmium-fixed, Epon-embedded tissues. J. Electron Micmsc. 43: 361-366.
7. Kingsley R E and Cole N L (1988) Preparation of cultured mammalian cells for transmission and scanning electron microscopy using Adar film. J. Electron Micmsc. Tech. 10: 77-85.
8. Masurovsky E B and Bunge R P (1989) Aclar film in biological electron microscopy. J. Eleamn Micmsc. Tech. 12: 172-173.
9. Kraft L M, Joyce K and D'Amelio E D (1983) Removal of histological sections from glass for electron microscopy: use of Quetol 651 resin and heat. Stain Tech. 58 41-43.