Selective activation of sar promoters with the use of green fluorescent protein transcriptional fusions as the detection system in the rabbit endocarditis model

Infection and Immunity

Volumn 66, Issue 12, 1998, Pages 5988-5993

  Cheung, A L ^a   Nast, Cynthia C ^b,c   Bayer, A S ^c,d  

^a ROCKEFELLER UNIVERSITY   (United States)

^b CEDARS SINAI MEDICAL CENTER   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d St John's Cardiovascular Research Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; PLASMID VECTOR;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL VIRULENCE; ENDOCARDITIS; FLUORESCENCE MICROSCOPY; GENE ACTIVATION; GENE CONTROL; IMMUNOBLOTTING; MOUSE; NONHUMAN; NORTHERN BLOTTING; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; RABBIT; REPORTER GENE; STAPHYLOCOCCUS AUREUS;

EID: 0031742107     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.66.12.5988-5993.1998     Document Type: Article

References (30)

1. Bayer, A. S., M. D. Ramos, B. E. Menzies, M. R. Yeaman, A. Shen, and A. L. Cheung. 1997. Hyperproduction of alpha-toxin by Staphylococcus aureus results in paradoxically reduced virulence in experimental endocarditis: host defense role for platelet microbicidal protein. Infect. Immun. 65:4652-4660.
2. Bayer, M. G., J. H. Heinrichs, and A. L. Cheung. 1996. The molecular architecture of the sar locus in Staphylococcus aureus. J. Bacteriol. 178:4563-4570.
3. Blake, M. S., K. H. Johnston, G. J. Russell-Jones, and E. C. Gotschlich. 1984. A rapid sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots. Anal. Biochem. 136:175-179.
4. Boyce, J. M. 1997. Epidemiology and prevention of nosocomial infections, p. 309-329. In K. B. Crossley and G. L. Archer (ed.), The staphylococci in human disease. Churchill Livingstone, New York, N.Y.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248.
6. Cheung, A. L., K. Eberhardt, and V. A. Fischetti. 1994. A method to isolate RNA from gram-positive bacteria and Mycobacteria. Anal. Biochem. 222: 511-514.
7. - mutant of Staphylococcus aureus in the rabbit model of endocarditis. J. Clin. Invest. 94:1815-1822.
8. Cheung, A. L., J. M. Koomey, C. A. Butler, S. J. Projan, and V. A. Fischetti. 1992. Regulation of exoprotein expression in Staphylococcus aureus by a locus (sar) distinct from agr. Proc. Natl. Acad. Sci. USA 89:6462-6466.
9. Cheung, A. L., and S. J. Projan. 1994. Cloning and sequencing of sarA: a gene required for the expression of agr. J. Bacteriol. 176:4168-4172.
10. Chien, Y., and A. L. Cheung. 1998. Molecular interactions between two global regulators, sar and agr, in Staphylococcus aureus. J. Biol. Chem. 237: 2645-2652.
11. Chien, Y., A. C. Manna, and A. L. Cheung. SarA level is a determinant of agr activation in Staphylococcus aureus. Mol. Microbiol., in press.
12. Clontech Inc. Unpublished data.
13. Cohen, M. L. 1992. Epidemiology of drug resistance: implications for a post-antibiotic era. Science 257:1050-1055.
14. Compagnone-Post, P., U. Malyankar, and S. A. Khan. 1991. Role of host factors in the regulation of the enterotoxin B gene. J. Bacteriol. 173:1827-1830.
15. SB of Staphylococcus aureus: characterization and role in transcription of the global regulatory locus sar. J. Bacteriol. 179:6355-6359.
16. Durack, D. T. 1975. Experimental bacterial endocarditis. IV. Structure and evolution of very early lesions. J. Clin. Pathol. 45:81-89.
17. Gaskill, M. E., and S. A. Khan. 1988. Regulation of the enterotoxin B gene in Staphylococcus aureus. J. Biol. Chem. 263:6276-6280.
18. Heinrichs, J. H., M. G. Bayer, and A. L. Cheung. 1996. Characterization of the sar locus and its interaction with agr in Staphylococcus aureus. J. Bacteriol. 178:418-423.
19. Janzon, L., and S. Arvidson. 1990. The role of the δ-hemolysin gene (hld) in the regulation of virulence genes by the accessory gene regulator (agr) in Staphylococcus aureus. EMBO J. 9:1391-1399.
20. Kornblum, J., B. Kreiswirth, S. J. Projan, H. Ross, and R. P. Novick. 1990. Agr: a polycistronic locus regulating exoprotein synthesis in Staphylococcus aureus, p. 373-402. In R. P. Novick (ed.), Molecular biology of the staphylococci, VCH Publishers, New York, N.Y.
21. Mahmood, R., and S. A. Khan. 1990. Role of upstream sequences in the expression of the staphylococcal enterotoxin B gene. J. Biol. Chem. 265: 4652-4656.
22. Manna, A. C., M. G. Bayer, and A. L. Cheung. 1998. Transcriptional analysis of different promoters in the sar locus in Staphylococcus aureus. J. Bacteriol. 180:3828-3836.
23. Novick, R. P. 1990. The staphylococcus as a molecular genetic system, p. 1-40. In R. P. Novick (ed.), Molecular biology of the staphylococci. VCH, New York, N.Y.
24. Projan, S. J., and R. P. Novick. 1997. The molecular basis of pathogenicity, p. 55-81. In K. B. Crossley and G. L. Archer (ed.). The staphylococci in human diseases. Churchill Livingston, New York, N.Y.
25. Ray, C., R. E. Hay, H. L. Carter, and C. P. J. Moran. 1985. Mutations that affect utilization of a promoter in stationary-phase Bacillus subtilis. J. Bacteriol. 163:180-185.
26. Regassa, L., R. P. Novick, and M. J. Betley. 1992. Glucose and nonmaintained pH decrease expression of accessory gene regulator (agr) in Staphylococcus aureus. Infect. Immun. 60:3381-3388.
27. Schenk, S., and R. A. Laddaga. 1992. Improved method for electroporation of Staphylococcus aureus. FEMS Microbiol. Lett. 94:133-138.
28. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
29. Waldvogel, F. A. 1985. Staphylococcus aureus, p. 1097-1116. In G. L. Mandell, R. G. J. Douglas, and J. E. Bennett (ed.), Principles and practice of infectious diseases, John Wiley & Sons, New York, N.Y.
30. Zukowski, M. M., D. G. Gaffney, D. Speck, M. Kauffman, A. Findeli, A. Wisecup, and J. P. Lecocq. 1983. Chromogenic identification of genetic regulatory signals in Bacillus subtilis based on expression of a cloned Pseudomonas gene. Proc. Natl. Acad. Sci. USA 80:1101-1105.