Role of Cx43 Phosphorylation and MAP Kinase Activation in EGF Induced Enhancement of Cell Communication in Human Kidney Epithelial Cells

Cell Communication and Adhesion

Volumn 5, Issue 6, 1998, Pages 451-460

  Vikhamar, Gunnhild ^a   Rivedal, Edgar ^a   Mollerup, Steen ^b   Sanner, Tore ^a  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

^b NATIONAL INSTITUTE OF OCCUPATIONAL HEALTH   (Norway)

Author keywords

Connexin43; EGF; MAP kinase; Phorbol ester; Phosphorylation

Indexed keywords

CONNEXIN 43; EPIDERMAL GROWTH FACTOR; MITOGEN ACTIVATED PROTEIN KINASE; PHORBOL 13 ACETATE 12 MYRISTATE; PHOSPHOSERINE; PHOSPHOTYROSINE;

ARTICLE; CELL COMMUNICATION; CONTROLLED STUDY; ENZYME ACTIVATION; GAP JUNCTION; HUMAN; HUMAN CELL; KIDNEY EPITHELIUM; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

ANTIBODIES; BLOTTING, WESTERN; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CELL COMMUNICATION; CELL LINE; CONNEXIN 43; ENZYME ACTIVATION; EPIDERMAL GROWTH FACTOR; EPITHELIAL CELLS; GAP JUNCTIONS; HUMANS; KIDNEY; PHOSPHOPROTEINS; PHOSPHORYLATION; PHOSPHOSERINE; PHOSPHOTYROSINE; PRECIPITIN TESTS; TETRADECANOYLPHORBOL ACETATE; TIME FACTORS;

EID: 0031740669     PISSN: 10615385     EISSN: None     Source Type: Journal    
DOI: 10.3109/15419069809005603     Document Type: Article

References (28)

1. Carpenter, G. and Cohen, S (1990). Epidermal growth factor [Review]. J. Biol. Chem. 265, 7709-7712.
2. Caveney, S. (1985). The role of gap junctions in development [Review] Annu. Rev. Physiol. 47, 319-335.
3. Diez, J.A., Elvira, M. and Villalobo, A. (1995). Phosphorylation of connexin-32 by the epidermal growth factor receptor tyrosine kinase. Ann N Y. Acad. Sci. 166, 477-480
4. Goodenough, D.A. and Musil, L.S. (1993). Gap junctions and tissue business: problems and strategies for developing specific functional reagents [Review]. J Cell Sci Suppl. 17, 133-138.
5. Hill, C.S., Oh, S.Y., Schmidt, S.A., Clark, K.J. and Murray, A.W. (1994). Lysophosphatidic acid inhibits gap-junctional communication and stimulates phosphorylation of connexin-43 in WB cells possible involvement of the mitogen-activated protein kinase cascade Biochem. J. 303, 475-479.
6. Hunter, T and Sefton, B M. (1980) Transforming gene product of Rous sarcoma virus phosphorylates tyrosine. Proc. Natl. Acad. Sci. USA 77, 1311-1315.
7. Kanemitsu, M.Y. and Lau, A.F. (1993) Epidermal growth factor stimulates the disruption of gap junctional communication and connexin43 phosphorylation independent of 12-O-tetradecanoylphorbol 13-acctate-sensitive protein kinase C: the possible involvement of mitogen-activated protein kinase. Mol. Biol. Cell 4, 837-848.
8. Kikkawa, U., Kishimoto, A and Nishizuka, Y. (1989). The protein kinase C family: Heterogeneity and its implications Annu. Rev. Biochem. 58, 31-44.
9. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bactenophage T4. Nature 227, 680-685.
10. Lau, A.F., Kanemitsu, M.Y., Kurata, W.E., Danesh, S. and Boynton, A.L. (1992). Epidermal growth factor disrupts gap-junctional communication and induces phosphorylation of connexin43 on serine. Mol. Biol. Cell 3, 865-874
11. Loewenstein, W.R. (1979). Junctional intercellular communication and the control of growth [Review]. Biochim. Biophys. Acta 560, 1-65.
12. Loo, L.W., Berestecky, J.M., Kanemitsu, M.Y. and Lau, A.F. (1995). pp60src-mediated phosphorylation of connexin43, a gap junction protein. J. Biol. Chem. 270, 12751-12761.
13. Madhukar, B.V., Oh, S.Y., Chang, C.C., Wade, M. and Trosko, J.E. (1989). Altered regulation of intercellular communication by epidermal growth factor, transforming growth factor-beta and peptide hormones in normal human keratinocytes Carcinogenesis 10, 13-20.
14. Maldonado, P E., Rose, B. and Loewenstein, W.R. (1988). Growth factors modulate junctional cell-to-cell communication. J. Membr. Biol. 106, 203-210.
15. Mikalsen, S.O., Husoy, T., Vikhamar, G. and Sanner, T. (1997). Induction of phosphotyrosine in the gap junction protein, connexin43. FEBS Lett. 401, 271-275.
16. Mollerup, S., Rivedal, E., Moehle, L. and Haugen, A. (1996). Nickel(II) induces alterations in EGF- and TGF-beta 1-mediated growth control during malignant transformation of human kidney epithelial cells. Carcinogenesis 17, 361-367.
17. Musil, L.S. and Goodenough, D.A. (1991). Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques. J. Cell Biol. 115, 1357-1374
18. Nakamura, K., Zhou, C.J., Parente, J. and Chew, C.S. (1996). Parietal cell MAP kinases multiple activation pathways. Am. J Physiol. 271, G640-9.
19. Oh, S.Y , Grupen, C.G. and Murray, A.W. (1991). Phorbol ester induces phosphorylation and down-regulation of connexin 43 in WB cells. Biochim. Biophys. Acta 1094, 243-245.
20. Oh, S.Y., Schmidt, S.A. and Murray, A.W. (1993). Epidermal growth factor inhibits gap junctional communication and stimulates serine-phosphorylation of connexin43 in WB cells by a protein kinase C-independent mechanism. Cell Adhes. Commun. 1, 143-149.
21. Rivedal, E., Yamasaki, H. and Sanner, T. (1994). Inhibition of gap junctional intercellular communication in Syrian hamster embryo cells by TPA, retinoic acid and DDT. Carcinogenesis 15, 689-694.
22. Rivedal, E., Mollerup, S., Haugen, A. and Vikhamar, G. (1996). Modulation of gap junctional intercellular communication by EGF in human epithelial cells. Carcinogenesis 17, 2321-2328.
23. Saez, J C., Berthoud, V M., Moreno, A.P. and Spray, D.C (1993) Gap junctions. Multiplicity of controls in differentiated and undifferentiated cells and possible functional implications [Review]. Adv. Second Messenger Phosphoprotein Res. 27, 163-198
24. Tveito, G., Hansteen, I.L., Dalen, H. and Haugen, A (1989). Immortalization of normal human kidney epithelial cells by nickel(II). Cancer Res. 49, 1829-1835.
25. Ueda, Y., Hirai, S., Osada, S., Suzuki, A., Mizuno, K. and Ohno, S. (1996). Protein kinase C activates the MEK-ERK pathway in a manner independent of Ras and dependent on Raf. J. Biol. Chem. 271, 23512-23519.
26. Ullrich, A. and Schlessinger, J. (1990). Signal transduction by receptors with tyrosine kinase activity [Review]. Cell 61, 203-212.
27. Warn-Cramer, B.J., Lampe, P.D., Kurata, W.E., Kanemitsu, M.Y., Loo, L.W., Eckhart, W. and Lau, A.F. (1996). Characterization of the mitogen-activated protein kinase phosphorylation sites on the connexin-43 gap junction protein. J. Biol. Chem. 271, 3779-3786.
28. Xie, H.Q. and Hu, V.W. (1994). Modulation of gap junctions in senescent endothelial cells. Exp. Cell Res. 214, 172-176.