An immunohistochemical and in situ hybridisation study of the postnatal development of uncoupling protein-1 and uncoupling protein-1 mRNA in lamb perirenal adipose tissue

Cell and Tissue Research

Volumn 294, Issue 3, 1998, Pages 461-466

  Finn, David ^a   Lomax, Michael A ^a   Trayhurn, Paul ^b  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

^b ROWETT RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Brown adipose tissue; Lamb; mRNA; Thermogenesis; Uncoupling protein 1; White adipose tissue

Indexed keywords

MESSENGER RNA; UNCOUPLING PROTEIN;

ANIMAL TISSUE; ARTICLE; BROWN ADIPOSE TISSUE; CONTROLLED STUDY; GENE EXPRESSION; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; LAMB; NONHUMAN; POSTNATAL DEVELOPMENT; PRIORITY JOURNAL; PROTEIN CONTENT; THERMOGENESIS; WHITE ADIPOSE TISSUE;

ADIPOSE TISSUE; ANIMALS; CARRIER PROTEINS; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; ION CHANNELS; KIDNEY; MEMBRANE PROTEINS; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; RNA, MESSENGER; SHEEP;

EID: 0031739796     PISSN: 0302766X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004410051197     Document Type: Article

References (26)

1. Cannon B, Nedergaard (1985) The biochemistry of an inefficient tissue - brown adipose-tissue. Essays Biochem 20:110-164
2. Casteilla L, Forest C, Robelin J, Ricquier D, Lombet A, Ailhaud G (1987) Characterization of mitochondrial-uncoupling protein in bovine fetus and newborn calf. Am J Physiol 252:E627-E636
3. Casteilla L, Champigny O, Bouillaud F, Robelin J, Ricquier D (1989) Sequential changes in the expression of mitochondrial protein mRNA during the development of brown adipose-tissue in bovine and ovine species - sudden occurrence of uncoupling protein mRNA during embryogenesis and its disappearance after birth. Biochem J 257:665-671
4. Casteilla L, Nougas J, Reyne Y, Ricquier D (1991) Differentiation of ovine brown adipocyte precursor cells in a chemically defined serum-free medium. Eur J Biochem 198:195-199
5. Champigny O, Ricquier D, Blonde LO, Mayers RM, Briscoe MG, Holloway BR (1991) Beta-3-adrenergic receptor stimulation restores message and expression of brown-fat mitochondrial uncoupling protein in adult dogs. Proc Natl Acad Sci USA 88:10774-10777
6. Champigny O, Holloway BR, Ricquier D (1992) Regulation of UCP gene-expression in brown adipocytes differentiated in primary culture - effects of a new beta-adrenoceptor agonist. Mol Cell Endocrinol 86:73-82
7. Clarke L, Bird JA, Lomax MA, Symonds ME (1996) Effect of beta(3)-adrenergic agonist (Zeneca D7114) on thermoregulation in near-term lambs delivered by cesarean-section. Pediatr Res 40:330-336
8. Cousin B, Cinti S, Morroni M, Raimbault S, Ricquier D, Penicaud L, Casteilla L (1992) Occurrence of brown adipocytes in rat white adipose-tissue - molecular and morphological characterization. J Cell Sci 103:931-942
9. Darby CJ, Clarke L, Lomax MA, Symonds ME (1996) Brown adipose-tissue and liver development during early postnatal life in hand-reared and ewe-reared lambs. Reprod Fert Devel 8:137-145
10. Gemmell RT, Bell AW, Alexander G (1972) Morphology of adipose cells in lambs at birth and during subsequent transition of brown to white adipose tissue in cold and warm condtions. Am J Anat 133:143-163
11. Giralt M, Martin I, Iglesias R, Vinas O, Villarroya F, Mampel (1990) Ontogeny and perinatal modulation of gene-expression in rat brown adipose-tissue - unaltered iodothyronine 5′-deiodinase activity is necessary for the response to environmental-temperature at birth. Eur J Biochem 193:297-302
12. Hsu SM, Raine L, Fanger H (1981a) A comparative-study of the peroxidase-antiperoxidase method and an avidin-biotin complex method for studying polypeptide hormones with radioimmunoassay antibodies. Am J Clin Pathol 75:734-738
13. Hsu SM, Raine L, Fanger H (1981b) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques - a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577-580
14. Komminoth P (1996) Detection of mRNA in tissue sections using DIG labelled RNA and oligonucleotide probes. Nonradioactive In Situ Hybridization Application Manual, 2nd edn, pp 126-131
15. Milner RE, Trayhurn P (1989) Rapid quantitation of uncoupling protein in brown adipose tissue mitochondria by a dot immunobinding ("dot blot") procedure: application to the measurement of uncoupling protein in Richardson's ground squirrel, rats and mice. Biochem Cell Biol 68:973-979
16. Moriscot A, Rabelo R, Bianco AC (1993) Corticosterone inhibits uncoupling protein gene-expression in brown adipose-tissue. Am J Physiol 265:E81-E87
17. Nicholls DG, Locke RM (1984) Thermogenic mechanisms in brown fat. Physiol Rev 64:1-64
18. Papaconstantinou J, Rabek JP, Zhang DE (1990) Molecular mechanisms of liver specific albumin and alpha-fetoprotein gene-regulation - a review: Develop Growth Different 32:205-216
19. Puigserver P, Herron D, Gianotli M, Palou A, Cannon B, Nedergaard J (1992) Induction and degradation of the uncoupling protein thermogenin in brown adipocytes in vitro and in vivo - evidence for a rapidly degradable pool. Biochem J 284:393-398
20. Rehnmark S, Kopecky J, Jacobsson A, Nechad M, Herron D, Nelson BD, Obregon MJ, Nedergaard J, Cannon B (1989) Brown adipocytes differentiated in vitro can express the gene for the uncoupling protein thermogenin - effects of hypothyroidism and norepinephrine. Exp Cell Res 182:75-83
21. Soppela P, Nieminen M, Saarela S, Keith JS, Morrison JN, Macfarlane F, Trayhurn P (1991) Brown fat-specific mitochondrial uncoupling protein in adipose tissues of newborn reindeer. Am J Physiol 260:R1229-R1234
22. Trayhurn P (1993) Brown adipose-tissue - from thermal physiology to bioenergetics. J Biosci 18:161-173
23. Trayhurn P, Duncan JS (1994) Rapid chemiluminescent detection of the messenger RNA for uncoupling protein in brown adiposetissue by Northern hybridization with a 32-mer oligonucleotide end-labeled with digoxigenin. Int J Obesity 8:449-452
24. Trayhurn P, Thomas MEA, Keith JS (1993a) Postnatal-development of uncoupling protein, uncoupling protein inRNA, and GLUT4 in adipose tissues of goats. Am J Physiol 265:R676-R 682
25. Trayhurn P, Thomas MEA, Duncan JS, Nicol F, Arthur JR (1993b) Presence of the brown fat-specific mitochondrial uncoupling protein and iodothyronine 5′-deiodinase activity in subcutaneous adipose tissue of neonatal lambs. FEBS Lett 322:76-78
26. Trayhurn P, Duncan JS, Nestor A, Thomas MEA, Rayner DV (1994) Chemiluminescent detection of mRNAs on Northern blots with digoxigenin end-labeled oligonucleotides. Anal Biochem 222:224-230