Elimination of residual natural nucleotides from 3'-O-modified-dNTP syntheses by enzymatic Mop-Up

BioTechniques

Volumn 25, Issue 5, 1998, Pages 814-817

  Metzker, Michael L ^a,d   Raghavachari, Ramesh ^b   Burgess, Kevin ^c   Gibbs, Richard A ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b LI COR BIOSCIENCES   (United States)

^c TEXAS A AND M UNIVERSITY   (United States)

^d MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; NUCLEOTIDE;

ARTICLE; CONTAMINATION; ENZYME ASSAY; ENZYME SPECIFICITY; NUCLEOTIDE SEQUENCE; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

EID: 0031739592     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98255st01     Document Type: Article

References (3)

1. Canard, B., B. Cardona and R.S. Sarfati. 1995. Catalytic editing properties of DNA polymerases. Proc. Natl. Acad. Sci. USA 92:10859-10863.
2. Canard, B. and R.S. Sarfati. 1994. DNA polymerase fluorescent substrates with reversible 3′-tags. Gene 148:1-6.
3. Metzker, M.L., R. Raghavachari, S. Richards, S.E. Jacutin, A. Civitello, K. Burgess and R.A. Gibbs. 1994. Termination of DNA synthesis by novel 3′-modified-deoxyribonucleoside 5′-triphosphates. Nucleic Acid Res. 22:4259-4267.