Conidia as a substrate for internal transcribed spacer-based PCR identification of members of the Leptosphaeria maculans species complex

Phytopathology

Volumn 88, Issue 11, 1998, Pages 1210-1217

  Balesdent, M H ^a   Jedryczka, M ^b   Jain, L ^c   Mendes Pereira, E ^a   Bertrandy, J ^a   Rouxel, T ^a  

^a CEMAGREF   (France)

^b INSTITUTE OF PLANT GENETICS   (Poland)

^c CETIOM   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CULTURE TECHNIQUE; DENATURATION; DISEASE DETECTION; DNA EXTRACTION; FUNGUS IDENTIFICATION; INTERNAL TRANSCRIBED SPACER REGION; ISOLATION PROCEDURE; PLANT FUNGUS INTERACTION; POLYMERASE CHAIN REACTION;

ASCOMYCOTA; BRASSICA NAPUS; ERYSIMUM; FUNGI; LEPIDIUM; LEPTOSPHAERIA; LEPTOSPHAERIA MACULANS; MYXOGASTRIA; PHOMA; PHOMA LINGAM; PHOMA NIGRIFICANS; SINAPIS ARVENSIS; THLASPI;

EID: 0031735298     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO.1998.88.11.1210     Document Type: Article

References (29)

1. Ansan-Melayah, D., Balesdent, M. H., Buée, M., and Rouxel, T. 1995. Genetic characterization of AvrLml, the first avirulence gene of Leptosphaeria maculons. Phytopathology 85:1525-1529.
2. Ansan-Melayah, D., Rouxel, T., Bertrandy, J., Letamec, B., Mendes-Pereira, E., and Balesdent, M. H. 1997. Field efficiency of Brassica napus specific resistance correlates with Leptosphaeria maculons population structure. Eur. J. Plant Pathol. 103:835-841.
3. Aufauvre-Brown, A., Tang, C. M., and Holden, D. W. 1993. Detection of gene-disruption events in Aspergillus transformants by polymerase chain reaction direct from conidiospores. Curr. Genet. 24:177-178.
4. Balesdent, M. H., Gall, C., Robin, P., and Rouxel, T. 1992. Intra-specific variation in soluble mycelial protein and esterase patterns of Leptosphaeria maculons French isolates. Mycol. Res. 96:677-684.
5. Dellaporta, S. L., Wood, J., and Hicks, J. B. 1983. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep. 1:19-21.
6. Ferreira, A. V. B., and Glass, N. L. 1996. PCR from fungal spore after microwave treatment. Fungal Genetics Newsletter 43. University of Kansas Medical Center, Kansas City.
7. 0 Leptosphaeria maculans isolates as revealed by soluble protein and isozyme electrophoresis. Mycol. Res. 99:221-229.
8. Goodwin, P. H., and Annis, S. L. 1991. Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assay. Appl. Environ. Microbiol. 57:2482-2486.
9. Jedryczka, M., Fitt, B. D. L., Kachlicki, P., Lewartowska, E., Balesdent, M. H., and Rouxel, T. A comparison between Polish and UK isolates of Leptosphaeria maculans, cause of stem canker of winter oilseed rape. IOCB Bull. In press.
10. Jedryczka, M., Lewartowska, E., and Frencel, I. 1995. Is Phoma nigrificans the potential pathogen of oilseed rape? Pages 625-627 in: Proc. Int. Rapeseed Congr., 9th. GCRIC (Groupe Consultatif International de Recherche sur le Colza), Paris.
11. Koch, E., Song, K., Osborn, T. C., and Williams, P. H. 1991. Relationship between pathogenicity and phylogeny based on restriction fragment length polymorphism in Leptosphaeria maculans. Mol. Plant-Microbe Interact. 4:341-349.
12. Leclair, S., Ansan-Melayah, D., Rouxel, T., and Balesdent, M. H. 1996. Meiotic behaviour of the minichromosome in the phytopathogenic as-comycete Leptosphaeria maculans. Curr. Genet. 30:541-548.
13. Louanchi, M. 1993. Contribution à l'étude de Rigidoporus lignosus, agent du pourridié blanc des racines d'Hevea brasiliensis: Étude de la diversité génétique des populations et détection par les méthodes immunoenzymatiques. Ph.D. thesis. University Paris-Sud-Orsay, France.
14. Marcinkowska, J., and De Gruyter, J. 1996. Phoma nigrificans (P. Karst.) Boerema et al., a new species for Poland. J. Phytopathol. 144:53-54.
15. Meyer, W., Lieckfeldt, E., Wöstemeyer, J., and Borner, T. 1992. DNA fingerprinting for differentiating aggressivity groups of the rape seed pathogen Leptosphaeria maculans. Mycol. Res. 96:651-657.
16. Morales, V. M., Jasalavich, C. A., Pelcher, L. E., Petrie, G. A., and Taylor, J. L. 1995. Phylogenetic relationship among several Leptosphaeria species based on their ribosomal DNA sequences. Mycol. Res. 99:593-603.
17. Morales, V. M., Pelcher, L. E., and Taylor, J. L. 1993. Comparison of the 5.8s rDNA and internal transcribed spacer sequences of isolates of Leptosphaeria maculans from different pathogenicity groups. Curr. Genet. 23:490-495.
18. Pedras, M. S. C. 1995. Phytoalexin detoxification and phytotoxin biosynthesis in blackleg fungi. Blackleg News 5:2-4.
19. Petrie, G. A. 1969. Variability in Leptosphaeria maculans (Desm.) Ces. & De Not., the cause of blackleg of rape. Ph.D. thesis. University of Saskatoon, Saskatoon, Canada.
20. Plummer, K. M., Dunse, K., and Hewlett, B. J. 1994. Non-aggressive strains of the blackleg fungus, Leptosphaeria maculans, are present in Australia and can be distinguished from aggressive strains by molecular analysis. Aust. J. Bot. 42:1-8.
21. Plummer, K. M., and Hewlett, B. J. 1993, Major chromosome length polymorphisms are evident after meiosis in the phytopathogenic fungus Leptosphaeria maculons. Curr. Genet. 24:107-113.
22. Rouxel, T., Balesdent, M. H., Séguin-Swartz, G., and Gugel, R. 1995. How many pathogens cause blackleg of crucifers? Blackleg News 4:1-7.
23. Rouxel, T., Gall, C., and Balesdent, M. H. 1994. Du polymorphisme au complexe d'espèces: Combien d'agents pathogènes sont impliqués dans la nécrose du collet du colza? Agronomie 14:413-432.
24. Rouxel, T., and Séguin-Swartz, G. 1995. International blackleg of crucifers network. Blackleg News 5:15-16.
25. Schäfer, C., and Wöstemeyer, J. 1992. Random primer dependent PCR differentiate aggressive from non-aggressive isolates of the oilseed rape pathogen Phoma lingam (Leptosphaeria maculons). J. Phytopathol. 136: 124-136.
26. Séguin-Swartz, G. 1994. International blackleg network. Blackleg News 4:7.
27. White, T. J., Bruns, T., Lee, S., and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pages 315-322 in: PCR Protocols. A Guide to Methods and Applications. M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White, eds. Academic Press, Inc., San Diego, CA.
28. Xu, J. R., and Hamer, J. E. 1995. Assessment of Magnaporthe grisea mating type by spore PCR. Fungal Genetics Newsletter 42. University of Kansas Medical Center, Kansas City.
29. Xue, B., Goodwin, P. H., and Annis, S. L. 1992. Pathotype identification of Leptosphaeria maculans with PCR and oligonucleotide primers from ribosomal internal transcribed spacer sequences. Physiol. Mol. Plant Pathol. 41:179-188.