Sequence polymorphism in the β-tubulin gene reveals heterogeneous and variable population structures in Cryptosporidium parvum

Applied and Environmental Microbiology

Volumn 64, Issue 11, 1998, Pages 4477-4481

  Widmer, Giovanni ^a,d   Tchack, Laurie ^a   Chappell, Cynthia L ^b   Tzipori, Saul ^a,c  

^a TUFTS UNIVERSITY   (United States)

^b UNIVERSITY OF TEXAS SCHOOL OF PUBLIC HEALTH   (United States)

^c TUFTS MEDICAL CENTER   (United States)

^d TUFTS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA TUBULIN;

GENETIC ANALYSIS; METHODOLOGY; POPULATION STRUCTURE; PROTOZOA;

ARTICLE; CRYPTOSPORIDIUM PARVUM; GENE SEQUENCE; GENE STRUCTURE; GENETIC HETEROGENEITY; GENOTYPE; MICROBIOLOGICAL EXAMINATION; NONHUMAN; NUCLEOTIDE SEQUENCE; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEQUENCE ANALYSIS;

CRYPTOSPORIDIUM PARVUM; PROTOZOA;

EID: 0031734091     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.11.4477-4481.1998     Document Type: Article

References (18)

1. Awad-El-Kariem, F. M., H. A. Robinson, D. A. Dyson, D. Evans, S. Wright, T. M. Fox, and V. McDonald. 1995. Differentiation between human and animal strains of Cryptosporidium parvum using isoenzyme typing. Parasitology 110:129-132.
2. Awad-El-Kariem, F. M., H. A. Robinson, F. Petry, V. McDonald, D. Evans, and D. Casemore. 1998. Differentiation between human and animal isolates of Cryptosporidium parvum using molecular and biological markers. Parasitol. Res. 84:297-301.
3. Bonnin, A., M. N. Fourmaux, J. F. Dubremetz, R. G. Nelson, P. Gobet, G. Harly. M. Buisson, D. Puygauthier-Toubas, F. Gabriel-Pospisil, M. Naciri, and P. Camerlynck. 1996. Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction-restriction fragment length polymorphism analysis of a repetitive DNA sequence. FEMS Microbiol. Lett. 137:207-211.
4. Cacciò, S., G. La Rosa, and E. Pozio. 1997. The beta-tubulin gene of Cryptosporidium parvum. Mol. Biochem. Parasitol. 89:307-311.
5. Carraway, M., S. Tzipori, and G. Widmer. 1996. Identification of genetic heterogeneity in the Cryptosporidium parvum ribosomal repeat. Appl. Environ. Microhiol. 62:712-716.
6. Carraway, M., S. Tzipori, and G. Widmer. 1997. New restriction fragment length polymorphism marker in Cryptosporidium parvum identifies mixed parasite populations and genotypic instability in response to host change. Infect. Immun. 65:3958-3960.
7. Guerrant, R. L. 1997. Cryptosporidiosis: an emerging, highly infectious threat. Emerg. Infect. Dis. 3:51-57.
8. 7a. Le Blancq, S. (Columbia University). Personal communication.
9. Le Blancq, S. M., N. Khramtsov, F. Zamani, S. J. Upton, and T. W. Wu. 1997. Ribosomal RNA gene organization in Cryptosporidium parvum. Mol. Biochem. Parasitol. 90:463-178.
10. Morgan, U. M., C. C. Constantine, P. O'Donoghue, B. P. Meloni, P. A. O'Brien, and R. C. A. Thompson. 1995. Molecular characterization of Cryptosporidium isolates from human and other animals using random amplified polymorphic DNA analysis. Am. J. Trop. Med. Hyg. 52:559-564.
11. Peng, M. M., L. Xiao, A. R. Freeman, M. J. Arrowood, A. A. Escalante, A. C. Weltman, C. S. L. Ong, W. R. MacKenzie, A. A. Lal, and C. B. Beard. 1997. Genetic polymorphism among Cryptosporidium parvum isolates: evidence for two distinct human transmission cycles. Emerg. Infect. Dis. 3:567-573.
12. 10a. Piper, M. (Medical Research Council, Cambridge, United Kingdom). Personal communication.
13. Spano, F., L. Putignani, J. McLaughlin, D. P. Casemore, and A. Crisanti. 1997. PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin. FEMS Microbiol. Lett. 152: 209-217.
14. Tzipori, S. 1988. Cryptosporidiosis in perspective. Adv. Parasitol. 27:63-130.
15. Tzipori, S., W. Rand, J. Griffiths, G. Widmer, and J. Crabb. 1994. Evaluation of an animal model system for cryptosporidiosis: therapeutic efficacy of paromomycin and hyperimmune bovine colostrum-immunoglobulin. Clin. Diagn. Lab. Immunol. 1:450-463.
16. Widmer, G. 1998. Genetic heterogeneity and PCR detection of Cryptosporidium parvum. Adv. Parasitol. 40:224-241.
17. 14a. Widmer, G., L. Tchack, and S. Tzipori. Unpublished data.
18. Widmer, G., S. Tzipori, C. J. Fichtenbaum, and J. K. Griffiths. 1998. Genotypic and phenotypic characterization of Cryptosporidium parvum isolates from people with AIDS. J. Infect. Dis. 178:834-840.