Identification of a marine agarolytic Pseudoalteromonas isolate and characterization of its extracellular agarase

Applied and Environmental Microbiology

Volumn 64, Issue 11, 1998, Pages 4378-4383

  Vera, Jorge ^a   Alvarez, Raul ^a   Murano, Erminio ^b   Slebe, Juan Carlos ^a   Leon, Oscar ^a  

^a UNIVERSIDAD AUSTRAL DE CHILE   (Chile)

^b Laboratorio Biopolimeri Tecnologici   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

AGARASE; BACTERIAL ENZYME; RNA 16S; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; GEL ELECTROPHORESIS; GEL FILTRATION; GRAM NEGATIVE AEROBIC RODS AND COCCI; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PHENOTYPE; PHYLOGENY; PROTEIN PURIFICATION; PSEUDOALTEROMONAS; TEMPERATURE;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; PROKARYOTA; PSEUDOALTEROMONAS; PSEUDOALTEROMONAS ANTARCTICA; UNCULTURED MARINE BACTERIUM;

EID: 0031733570     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.11.4378-4383.1998     Document Type: Article

References (47)

1. Agbo, J. A. C., and M. O. Moss. 1979. The isolation and characterization of agarolytic bacteria from a lowland river. J. Gen. Microbiol. 115:355-368.
2. Akagawa-Matsushita, M., M. Matsuo, Y. Koga, and K. Yamasato. 1992. Alteromonas atlantica sp. nov. and Alteromonas carrageenovora sp. nov., bacteria that decompose algal polysaccharides. Int. J. Syst. Bacteriol. 42:621-627.
3. Aoki, T., T. Araki, and M. Kitamikado. 1990. Purification and characterization of a novel β-agarase from Vibrio sp. AP-2. Eur. J. Biochem. 187:461-465.
4. Ausubel, F., R. Brent, R. Kingston, D. Moore, J. Seidman, J. Smith, and K. Struhl. 1987. Current protocols in molecular biology, p. 2.4.1-2.4.2. John Wiley & Sons, Inc., New York, N.Y.
5. Barbeyron, H., and B. Kloareg. 1994. The gene encoding the kappa-carragenase of Alteromonas carrageenovora is related to β-1,3-1,4-glucanases. Gene 139:105-109.
6. Baumann, P., L. Baumann, M. Mandel, and R. Allen. 1972. Taxonomy of aerobic marine euhacteria. J. Bacteriol. 110:402-129.
7. Baumann, P., M. J. Gauthier, and L. Baumann. 1984. Genus Alteromonas Baumann, Baumann, Mandel and Allen 1972, p. 343-352. In N. R. Krieg and J. G. Holt (ed.), Bergey's manual of systematic bacteriology, vol. 1. The Williams & Wilkins Co., Baltimore, Md.
8. Belas, R., D. Bartlett, and M. Silverman. 1988. Cloning and gene replacement mutagenesis of a Pseudomonas atlantica agarase gene. Appl. Environ. Microbiol. 54:30-37.
9. Belas, R. 1989. Sequence analysis of the agrA gene encoding β-agarase from Pseudomonas atlantica. J. Bacteriol. 171:602-605.
10. Bibb, M., G. Jones, R. Joseph, M. Buttner, and J. Ward. 1987. The agarase gene (dagA) of Streptomyces coelicolor A3(2): affinity purification and characterization of the cloned gene product. J. Gen. Microbiol. 133:2089-2096.
11. Bozal, N., E. Tudela, R. Roselló-Mora, J. Lalucat, and J. Guinea. 1997. Pseudoalteromonas antarctica sp. nov. Int. J. Syst. Bacteriol. 47:345-351.
12. Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
13. Cole, R., and T. Popkin. 1981. Electron microscopy, p. 34-51. In P. Gerhardt (ed.), Manual of methods for general microbiology. American Society for Microbiology, Washington, D.C.
14. Cowan, S. 1974. Cowan and Steel's manual for the identification of medical bacteria, 2nd ed. Cambridge University Press, London, United Kingdom.
15. Day, D., and W. Yaphe. 1975. Enzymatic hydrolysis of agar, purification and characterization of neoagarobiose hydrolase and p-nitrophenyl-α-galactosidase hydrolase. Can. J. Microbiol. 21:1512-1518.
16. Duckworth, M., and J. R. Turvey. 1969. The specificity of an agarase from a Cytophaga species. Biochem. J. 113:693-697.
17. Dygert, S., H. L. Li, D. Florida, and J. A. Thoma. 1965. Determination of reducing sugar with improved precision. Anal. Biochem. 13:367-374.
18. Gauthier, M. J., and V. A. Breittmayer. 1992. The genera Alteromonas and Marinomonas, p. 3046-3070. In A. Balows, H. G. Trüper, M. Dworkin, W. Harder, and K. H. Schleifer (ed.), The prokaryotes, 2nd ed. Springer-Verlag, New York, N.Y.
19. Gauthier, M. J., B. Lafay, R. Christen, L. Fernandez, M. Acquaviva, P. Bonin, and J. C. Bertrand. 1992. Marinobacter hydrocarbonoclasticus gen. nov., sp. nov., a new, extremely halotolerant, hydrocarbon-degrading marine bacterium. Int. J. Syst. Bacteriol. 42:568-576.
20. Gauthier, G., M. Gauthier, and R. Christen. 1995. Phylogenetic analysis of the genera Alteromonas, Shewanella, and Moritella using genes coding for small-subunit rRNA sequences of the genus Alteromonas into two genera, Alteromonas (emended) and Pseudoalteromonas gen. nov., and proposal of twelve new species combinations. Int. J. Syst. Bacteriol. 45:755-761.
21. Groleau, D., and W. Yaphe. 1977. Enzymatic hydrolysis of agar: purification and characterization of neoagarotetraose hydrolase from Pseudomonas atlantica. Can. J. Microbiol. 23:672-679.
22. Ha, J., G. T. Kim, S. K. Kim, T. K. Oh, J. H. Yu, and I. S. Kong. 1997. β-Agarase from Pseudomonas sp. W7: purification of the recombinant enzyme from Escherichia coli and the effects of salt in its activity. Biotechnol. Appl. Biochem. 26:1-6.
23. Kumura, K., Y. Minamishima, S. Yamamoto, N. Ohashi, and A. Tamura. 1991. DNA base composition of Rickettsia tsutsugamushi determined by reversed-phase high-performance liquid chromatography. Int. J. Syst. Bacteriol. 41:247-248.
24. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
25. 13C-NMR spectroscopic investigation of methylated and charged agarose oligosaccharides and polysaccharides. Carbohydr. Res. 190:249-265.
26. Leifson, E. 1963. Determination of carbohydrate metabolism of marine bacteria. J. Bacteriol. 85:1183-1184.
27. Leon, O., L. Quintana, G. Peruzzo, and J. C. Slebe. 1992. Purification and properties of an extracellular agarase from Alteromonas sp. strain C-1. Appl. Environ. Microbiol. 58:4060-4063.
28. Macdonell, M. T., and R. R. Colwell. 1985. Phylogeny of the Vibrionaceae and recommendation for two new genera. Listonella and Shewanella. Syst. Appl. Microbiol. 6:171-182.
29. Morrice, L., M. McLean, F. Williamson, and W. Long. 1983. β-Agarases I and II from Pseudomonas atlantica: purification and some properties. Eur. J. Biochem. 135:553-558.
30. 13C-NMR spectroscopy. Eur. J. Biochem. 133:673-684.
31. Nozue, H., T. Hayashi, Y. Hashimoto, T. Ezaki, K. Hamasaki, K. Ohwada, and Y. Terawaki. 1992. Isolation and characterization of Shewanella alga from human clinical specimens and emendation of the description of S. alga Simidu et al., 1990, 335. Int. J. Syst. Bacteriol. 42:628-634.
32. Ortigosa, M., E. Garay, and M. J. Pujalte. 1994. Numerical taxonomy of aerobic, gram-negative bacteria associated with oysters and surrounding seawater of the mediterranean coast. Syst. Appl. Microbiol. 17:589-600.
33. Potin, P., C. Richard, C. Rochas, and B. Kloareg. 1993. Purification and characterization of the α-agarase from Alteromonas agarolyticus (Cataldi). Eur. J. Biochem. 214:599-607.
34. Rochas, C., P. Potin, and B. Kloareg. 1994. NMR spectroscopic investigation of agarose oligomers produced by an α-agarase. Carbohydr. Res. 253:69-77.
35. Ruimy, R., V. Breittmayer, P. Elbaze, B. Lafay, O. Boussemart, M. Gauthier, and R. Christen. 1994. Phylogenetic analysis and assessment of the genera Vibrio, Photobacterium, and Plesiomonas deduced from small-subunit rRNA sequences. Int. J. Syst. Bacteriol. 44:416-426.
36. Stanier, R. Y. 1942. Agar-decomposing strains of the Actinomyces coelicolor species group. J. Bacteriol. 44:555-570.
37. Stanier, R. Y., N. Palleroni, and M. Doudoroff. 1966. The aerobic pseudomonads: a taxonomic study. J. Gen. Microbiol. 43:159-271.
38. Stolp, H., and D. Gadkari. 1981. Nonpathogenic members of the genus Pseudomonas, p. 719-741. In M. P. Starr, H. Stolp, H. G. Trupper, A. Balows, and H. G. Schlegel (ed.), The prokaryotes: a handbook in habitats, isolation, and identification of bacteria. Springer-Verlag, New York, N.Y.
39. Sugano, Y., I. Terada, M. Arita, M. Noma, and T. Matsumoto. 1993. Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107. Appl. Environ. Microbiol. 59:1549-1554.
40. Sugano, Y., H. Kodama, and M. Noma. 1993. Cloning and sequencing of agaA, a unique agarase 0107 gene from a marine bacterium. Appl. Environ. Microbiol. 59:3750-3756.
41. Sugano, Y., and M. Noma. 1994. Sequence analysis of the agaB gene encoding a new agarase from Vibrio sp. strain JT 0107. Biochim. Biophys. Acta 1218:105-108.
42. Van der Meulen, H., and W. Harder. 1976. Characterization of the neoagarotetraose and neoagarobiase of Cytophaga flevensis. J. Microbiol. Serol. 42: 81-94.
43. Van der Meulen, H., and H. Vedkamp. 1974. Isolation and characterization of Cytophaga flevensis sp. nov., a new agarolytic flexibacterium. Antonie Leeuwenhoek 40:329-346.
44. Van Hofsteen, B., and M. Malmqvist. 1975. Degradation of agar by a gram negative bacterium. J. Gen. Microbiol. 87:150-158.
45. Yamaura, I., T. Matsumoto, M. Funatsu, H. Shigeiri, and T. Shibata. 1991. Purification and some properties of agarase from Pseudomonas sp. PT-5. Agric. Biol. Chem. 55:2531-2536.
46. Young, K., K. Hong, M. Duckworth, and W. Yaphe. 1971. Enzyme hydrolysis of agar and properties of bacterial agarases. Proc. Int. Seaweed Symp. 7:469-472.
47. Young, K., S. Bhattacharjee, and W. Yaphe. 1978. Enzymic cleavage of the alpha linkages in agarose to yield agarooligosaccharides. Carbohydr. Res. 66: 207-212.