Altered expression and modification of proteases from an avirulent mutant of Porphyromonas gingivalis W50 (W50/BE1)

Microbiology

Volumn 144, Issue 9, 1998, Pages 2487-2496

  Collinson, Lucy M ^a   Rangarajan, Minnie ^a   Curtis, Michael A ^a  

^a UNIVERSITY OF LONDON   (United Kingdom)

Author keywords

Altered modification; Avirulent; Extracellular arginine specific proteases; Porphyromonas gingivalis

Indexed keywords

BACTERIAL ENZYME; POLYPEPTIDE; PROTEINASE;

ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; NONHUMAN; PORPHYROMONAS GINGIVALIS; PRIORITY JOURNAL; SUPERNATANT;

ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; PORPHYROMONAS GINGIVALIS;

EID: 0031719946     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-144-9-2487     Document Type: Article

References (31)

1. Aduse-Opoku, J., Muir, J., Slaney, J. M., Rangarajan, M. & Curtis, M. (1995). Characterisation, genetic analysis and expression of a protease antigen (PrpR1) of Porphyromonas gingivalis W50. Infect Immun 63, 4744-4754.
2. Aduse-Opoku, J., Rangarajan, M., Young, K. A. & Curtis, M. A. (1998). Maturation of the arginine-specific proteases of Porphyromonas gingivalis W50 is dependent on a functional prR2 protease gene. Infect Immun 66, 1594-1600.
3. Chandad, F. & Mouton, C. (1995). Antigenic, structural, and functional relationships between fimbriae and the hemagglutinating adhesin HA-Ag2 of Porphyromonas gingivalis. Infect Immun 63, 4755-4763.
4. Curtis, M. A., Aduse-Opoku, J., Slaney, J. M., Rangarajan, M., Booth, V., Cridland, J. & Shepherd, P. (1996). Characterization of an adherence and antigenic determinant of the Argl protease of Porphyromonas gingivalis which is present on multiple geneproducts. Infect Immun 64, 2532-2539.
5. Cutler, C. W., Eke, P. I., Genco, C. A., VanDyke, T. E. & Arnold, R. R. (1996). Hemin-induced modifications of the antigenicity and hemin-binding capacity of Porphyromonas gingivalis lipopolysaccharide. Infect Immun 64, 2282-2287.
6. Dawson, R. M. C., Elliot, D. C., Elliot, W. H. & Jones, K. M. (editors) (1986). Data for Biochemical Research, 3rd edn. Oxford : Clarendon Press.
7. Fletcher, H. M., Schenkein, H. A., Morgan, R. M., Bailey, K. A., Berry, C. R. & Macrina, F. L. (1995). Virulence of a Porphyromonas gingivalis W83 mutant defective in the prtH gene. Infect Immun 63, 1521-1528.
8. Fox, T., de Miguel, E., Mort, J. S. & Storer, A. C. (1992). Potent slow-binding inhibition of cathepsin B by its propeptide. Biochemistry 31, 12571-12576.
9. Good, N. E., Winget, G. D., Winter, W., Connolly, T. N., Izawa, S. & Singh, R. M. M. (1966). Hydrogen ion buffers for biological research. Biochemistry 5, 467-477.
10. Han, N., Whitlock, J. & Progulske-Fox, A. (1996). The hemagglutinin gene A (hagA) of Porphyromonas gingivalis 381 contains four large, contiguous, direct repeats. Infect Immun 64, 4000-4007.
11. Hinode, D., Masuda, K., Yoshioka, M., Watanabe, K., Umemoto, T., Grenier, D., Mayrand, D. & Nakamura, R. (1995). Immunological characterization and localization of a Porphyromonas gingivalis BApNA-hydrolyzing protease possessing hemagglutinating activity. FEMS Microbiol Lett 131, 211-217.
12. Imamura, T., Potempa, J., Tanase, S. & Travis, J. (1997). Activation of blood coagulation factor X by arginine-specific cysteine proteinases (gingipain-R's) from Porphyromonas gingivalis. J Biol Chem 27, 16062-16067.
13. Kesavalu, L., Holt, S. C. & Ebersole, J. L. (1996). Trypsin-like protease activity of Porphyromonas gingivalis as a potential virulence factor in a murine lesion model. Microb Pathog 20, 1-10.
14. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
15. Lindsay, S. S., Wheeler, B., Sanderson, K. E. & Costerson, J. W. (1973). The release of alkaline phosphatase and of lipopolysaccharide during the growth of rough and smooth strains of Salmonella typhimurium. Can J Microbiol 19, 335-343.
16. McKee, A. S., McDermid, A. S., Wait, R., Baskerville, A. & Marsh, P. D. (1988). Isolation of colonial variants of Bacteroides gingivalis W50 with reduced virulence. J Med Microbiol 27, 59-64.
17. Nakayama, K., Kadowaki, T., Okamoto, K. & Yamamoto, K. (1995). Construction and characterization of arginine-specific cysteine proteinase (Arg-gingipain)-deficient mutants of Porphyromonas gingivalis. J Biol Chem 270, 23619-23626.
18. Nakayama, K., Yoshimura, F., Kadowaki, T. & Yamamoto, K. (1996). Involvement of arginine-specific cysteine proteinase (Arggingipain) in fimbriation of Porphyromonas gingivalis. J Bacteriol 178, 2818-2824.
19. Ohta, Y. & lnouye, M. (1990). Pro-subtilisin E: purification and characterization of its autoprocessing to active subtilisin E in vitro. Mol Microbiol 4, 295-304.
20. Okamoto, K., Kadowaki, T., Nakayama, K. & Yamamoto, K. (1996). Cloning and sequencing of the gene encoding a novel lysine-specific cysteine proteinase (Lys-gingipain) in Porphyromonas gingivalis: structural relationship with the argininespecific cysteine proteinase (Arg-gingipain). J Biochem 120, 398-406.
21. Pavloff, N., Potempa, J., Pike, R. N., Prochazka, V., Kiefer, M. C., Travis, J. & Barr, P. J. (1995). Molecular cloning and structural characterization of the Arg-gingipain proteinase of Porphyromonas gingivalis. J Biol Chem 270, 1007-1010.
22. Pike, R., McGraw, W., Potempa, J. & Travis, J. (1994). Lysine-and arginine-specific proteinases from Porphyromonas gingivalis. J Biol Chem 269, 406-411.
23. Pike, R. N., Potempa, J., McGraw, W., Coetzer, T. H. T. & Travis, J. (1996). Characterization of the binding activities of proteinaseadhesin complexes from Porphyromonas gingivalis. J Bacteriol 178, 2876-2882.
24. Potempa, J., Pike, R. & Travis, J. (1997). Titration and mapping of the active site of cysteine proteinases from Porphyromonas gingivalis (gingipains) using peptidyl chloromethanes. J Biol Chem 378, 223-230.
25. Rangarajan, M., Smith, S. J. M., U, S. & Curtis, M. A. (1997a). Biochemical characterization of the arginine-specific proteases of Porphyromonas gingivalis W50 suggests a common precursor. Biochem J 323, 701-709.
26. Rangarajan, M., Aduse-Opoku, J., Slaney, J. M., Young, K. A. & Curtis, M. A. (1997b). The prpR1 and prR2 arginine-specific proteases of Porphyromonas gingivalis W50 produce five biochemically distinct enzymes. Mol Microbiol 23, 955-965.
27. Shah, H. N., Seddon, S. V. & Gharbia, S. E. (1989). Studies on the virulence properties and metabolism of pleiotropic mutants of Porphyromonas gingivalis (Bacteroides gingivalis) W50. Oral Microbiol lmmunol 4, 19-23.
28. Smalley, J. W. & Birss, A. J. (1991). Extracellular vesicle-associated and soluble trypsin-like enzyme fractions of Porphyromonas gingivalis W50. Oral Microbiol lmmunol 6, 202-208.
29. Smalley, J. W., Birss, A. J., Kay, H. M., McKee, A. S. & Marsh, P. D. (1989). The distribution of trypsin-like enzyme activity in cultures of a virulent and an avirulent strain of Bacteroides gingivalis W50. Oral Microbiol lmmunol 4, 178-181.
30. van Steenbergen, T. J. N., Kastelein, P., Touw, J. J. A. & de Graff, J. (1982). Virulence of black-pigmented Bacteroides strains from periodontal pockets and other sites in experimentally induced skin lesions in mice. J Periodontal Res 17, 41-49.
31. Zhu, X., Ohta, Y., Jordan, F. & lnouye, M. (1989). Pro-sequence of subtilisin can guide the refolding of denatured subtilisin in an intramolecular process. Nature 339, 483-484.